XBridge Glycan BEH Amide, 130Å, 2.5 μm XP and 3.5 μm Columns and Standards
[ CARE AND USE MANUAL ]
1
CONTENTS
I.
INTRODUCTION
II.
GETTING STARTED
a. Column Installation
b. Column Equilibration
c. Initial Column Efficiency Determination
d. Conditioning of Previously
Unused Columns
e. Useful Functional Tests for
Benchmarking a New Column
f. Scalable UPLC and HPLC BEH Glycan
Column Offerings
III.
COLUMN USE
a. Sample Preparation
b. Operating pH Limit
c. Solvents
d. Pressure
e. Temperature
IV. TROUBLESHOOTING
V.
COLUMN CLEANING, REGENERATING,
AND STORAGE
a. Cleaning and Regeneration
b. Storage
VI.
CAUTIONARY NOTE
VII.
ORDERING INFORMATION
I. INTRODUCTION
Thank you for choosing a Waters® XBridge® Glycan BEH Amide,
130Å Guard or Column containing our 2.5 µm XP or 3.5 µm
particles designed for HILIC separations of 2-aminobenzamide
(2-AB), 2-aminobenzoic acid (2-AA), or Waters RapiFluor-MS™
(p/n: 176003635) labeled glycans. This column chemistry,
when used on an appropriately configured LC instrument,
is capable of separating both neutral and charged labeled
glycan species. Retention of 2-AB, 2-AA, or RapiFluor-MS
labeled oligosaccharides is based on the hydrophobicity of the
molecule, a parameter that is broadly related to hydrodynamic
volume or molecular size. The resolving power of these
columns is due in part to the particle size of the fully porous
packing materials. Chemical and mechanical stability of the
column are the consequence of Waters ethylene bridged hybrid
(BEH Technology™) particle composition.
The column may be calibrated using one of the labeled Waters
Dextran Calibration Ladder Standards (p/n: 186006841),
such that elution may be expressed in terms of glucose units
(GU). Under the suggested chromatographic conditions, the
retention of Waters RapiFluor-MS, 2-AB, or 2-AA labeled
oligosaccharides on the XBridge Glycan BEH Amide 2.5 µm XP
or 3.5 µm Column may be predicted based on the hydrophilic
contributions of the individual constituent monosaccharides.
XBridge Glycan BEH Amide, 130Å, 2.5 µm XP
and 3.5 µm Columns and Standards
2
XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards
[ CARE AND USE MANUAL ]
II. GETTING STARTED
Each XBridge Glycan BEH Amide Column has a Certificate
of Analysis and a Performance Test Chromatogram. The
Certificate of Analysis is specific to each batch of packing
material and includes the batch number and analyses of the
physical and chemical properties of the particle. Particle
size and pore structure are analyzed prior to bonding. The
carbon and nitrogen content of the bonding are measured
to insure consistent coverage. The selectivity of each batch
is also assessed with the chromatographic separation of
Waters Glycan Performance Standard (p/n: 186006349), a
2-AB labeled N-linked glycan standard mix spiked with Man-5
and Man-6, derived from human IgG. The complex mixture
of IgG glycans includes high mannose structures as well as
neutral and acidic complex structures. The retention times and
retention time differences of selected components are used as
the quality control test for each batch of packing material. The
Performance Test Chromatogram is specific to each individual
column and contains the following information: batch number,
column serial number, backpressure, USP plate count, reduced
plate height (RPH), USP tailing factor, retention factor (k�),
peak width, and chromatographic conditions. These data can
be found in the documentation supplied for each column and
should be stored for future reference.
a. Column Installation
Note: The flow rates given in the procedure below are for a typical 2.5 µm XP
packing in a 2.1 mm I.D. column. It is also highly recommended that a column
heater be used with our XBridge Glycan BEH Amide Column to help ensure
consistent and reproducible separated component retention times.
1. Purge the solvent delivery system of any buffer-containing
or water-immiscible mobile phases and connect the inlet
end of the column to the injector outlet. An arrow on the
column identification label indicates the correct direction
of solvent flow.
2. Flush the column with 100% organic mobile phase
(acetonitrile) by setting the pump flow to 0.1 mL/min
and increase the flow to 0.17 mL/min over 3 minutes.
Increase the aqueous phase to 90% over 15 minutes. Note
the backpressure. Decrease aqueous phase to starting
conditions (22% aqueous in the test chromatogram).
3. When the mobile phase is flowing freely from the column
outlet, stop the flow and attach the column outlet to the
detector. This prevents entry of air into the detection system
and gives more rapid baseline equilibration.
4. Gradually increase the flow rate from 0.17 to 0.34 mL/min
over 3 minutes.
5. Once a stable backpressure and baseline have been
achieved, proceed to the next section
b. Column Equilibration
Glycan Separation Technology Columns are shipped in
100% acetonitrile. It is important to ensure mobile phase
compatibility before changing to a different mobile-phase
system. Equilibrate the column with a minimum of 10 column
volumes of the mobile phase to be used (refer to Table 1 for
column volumes).
Table 1. Empty Column Volumes in mL
(multiply by 10 for flush solvent volumes)
Column Length
(mm)
Column Internal
Diameter (mm)
Empty Column
Volume (mL)
50
2.1
0.17
100
2.1
0.35
150
2.1
0.52
30
3.0
0.21
75
3.0
0.53
150
3.0
1.06
50
4.6
0.83
100
4.6
1.66
150
4.6
2.49
250
4.6
4.15
To avoid precipitating mobile-phase buffers on your column
or in your system, flush the column with five column volumes
of a water/organic solvent mixture using the same or higher
acetonitrile content as in the desired buffered mobile phase.
For example, flush the column and LC system with 50%
acetonitrile in water prior to introducing 50% acetonitrile/
50% buffered mobile phase.
The grade and quality of solvents are important for glycan
analysis. It is strongly recommended to use only LC-MS grade
reagents for glycan analysis mobile phase eluents. Use of
Waters ammonium formate solution (p/n: 186007081) is also
highly recommended in preparing Eluent A. It is a concentrate
made from LC-MS grade reagents and will reduce the
generation of undersired labeled glycan adducts.
Column equilibration may be judged initially by stable
pressure and by a stable detector baseline. For a specific
application, it is, however, necessary to test the required
duration of equilibration. The criteria for adequate
equilibration include reproducibility of retention time for
major and minor peaks, resolution for critical pairs, and
consistent baseline characteristics.
Note: Low concentration mobile-phase additives, particularly those
with minimal buffering capacity, may require extended equilibration
and re-equilibration between gradient analyses.
3
XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards
[ CARE AND USE MANUAL ]
c. Initial Column Efficiency Determination
1. Perform an efficiency test on the column before using
it in the desired application. Waters recommends using
the solute mixture and conditions described in the
“Performance Test Chromatogram” to test the column
upon receipt.
2. Measure the retention of the test compounds and the
number of theoretical plates (N).
3. Repeat the test at predetermined intervals to track column
performance over time. Slight variations may be obtained
on two different LC systems due to the quality of the
connections, operating environment, system electronics,
reagent quality, condition of column, and operator technique.
d. Conditioning of Previously Unused Columns
It is a good practice to ensure that new (previously unused)
Glycan BEH Amide, 130Å columns are well conditioned and
providing optimal performance before actual test sample
analyses. This can be accomplished via sequential injections
of a representative sample until a stable chromatographic
profile is achieved.
It should also be noted that even if a Glycan BEH Amide column
is to be used with an ammonium formate mobile phase, it can
still prove useful to first condition it with a gradient and mobile
phases containing 0.1% TFA. TFA can be effective in cleaning a
column’s stationary phase by both neutralizing and ion pairing
contaminants that in their ionic form might otherwise strongly
adsorb to a HILIC stationary phase.
e. Useful Functional Tests for Benchmarking
a New Column
We suggest use of Waters 2-AB labeled Glycan Performance
Standard (p/n: 186006349) to benchmark your new column
and monitor its performance during use. Note: This same 2-AB
Glycan Performance Standard is used by Waters manufacturing
to quality control test each batch of XBridge Glycan BEH Amide
130Å 2.5 µm XP and 3.5 µm material designed for this application
(See example in Figure 1).
To prepare the standard, add 100 µL of 50 mM ammonium
formate buffer pH 4.4 and 100 µL of acetonitrile directly to
the vial for a total volume of 200 µL.
Gently mix the sample by inversion.
Glycan Performance Test Standard
The separation shown in Figure 1 was generated on an
ACQUITY® UPLC® whose total system volume and post
column dispersion characteristics make it ideally suited
for this application. Note that similar results can be
obtained on an appropriately configured HPLC system. It
is also important to take note of the injection solvent and
injection volume for this application. Larger glycans have
limited solubility in solutions that contain more than 50%
acetonitrile. Gradual precipitation and loss of these larger
glycans will be observed under these condtions. However,
also note that large injections of water-containing samples
will distort the peak shape in HILIC chromatography.
Consequently, the optimum injection volume for these
applications is <3 µL.
4
XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards
[ CARE AND USE MANUAL ]
Figure 1. Typical chromatogram of 2-AB labeled human IgG N-linked glycans
using the Glycan Performance Test Standard (p/n: 186006349).
Note: Use of the scaled gradient shown below on an XBridge Glycan BEH
Amide, 3.5 µm 130Å, 2.1 x 150 mm Column (p/n: 186007504) should yield
results similar to Figure 1 that was generated on a XBridge Glycan BEH Amide,
130Å, 2.5 µm, XP, 2.1 x 150 mm Column (p/n: 186007265).
LC CONDITIONS
LC system:
ACQUITY UPLC H-Class Bio
Detection:
ACQUITY UPLC FLR Detector/
2475 FLR Detector
Excitation:
330 nm
Emission:
420 nm
Scan rate:
10 Hz
Time const.:
0.2 sec
Gain: 1.00
Column:
XBridge Glycan BEH Amide, 130Å,
2.5 µm XP, 2.1 x 150 mm (p/n: 186007265)
Column temp.: 60 °C
Sample temp.: 15 °C
Mobile phase A: 100 mM ammonium formate,
pH 4.5
Mobile phase B: Acetonitrile (ACN)
Vials:
LCGC Certified Clear Glass 12 x 32 mm
Screw Neck Qsert Vial (p/n: 186001126C)
Gradient:
Time
Flow Rate Profile
(min)
(mL/min)
%A
%B
0.00 0.34
22.00 78.00
56.62 0.34
44.10 55.90
58.09 0.1
80.00 20.007
65.44 0.17
80.00 20.00
68.38 0.34
22.00 78.00
75.53 0.34
22.00 78.00
Column:
XBridge Glycan BEH Amide, 130Å, 3.5 µm,
2.1 x 150 mm (p/n: 186007504)
Gradient:
Time
Flow Rate Profile
(min)
(mL/min)
%A
%B
0.00 0.24
22.00 78.00
79.26 0.20
44.10 55.904
81.32 0.12
80.00 20.00
91.62 0.12
80.00 20.00
95.74 0.24
22.00 78.00
102.94 0.24
22.00 78.00
Peak Identification
1. G0 (NGA2)
8. G1F+GN
2. G0F (NGA2F)
9. Man-6
3. Man-5
10. G2 (NA2)
4. G0F+GN
11. G2F (NA2F)
5. G1
12. G1F+SA
6. G1Fa (NA2G1F)
13. G2F+SA (A1F)
7. G1Fb (NA2G1F)
10
12
14
16
18
20
22
24
26
28
30 min
1
2
3
4
6
7
8 9
10
12
13
11
5
5
XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards
[ CARE AND USE MANUAL ]
f. Scalable UPLC and HPLC BEH Glycan
Column Offerings
Waters Glycan BEH Amide chemistry offerings are available
in three highly scalable particle sizes that address UPLC
(i.e. 1.7 µm) and HPLC-based (2.5 µm XP and 3.5 µm)
application needs. (See Waters ACQUITY UPLC Glycan
BEH Amide, 130Å, 1.7 µm Columns Care and Use
Manual, literature code: 720003042EN.) Figure 2 clearly
demonstrates the comparatively shorter analyses time with
improved component resolution as the particle size of the
XBridge Glycan BEH Amide Column chemistry is decreased.
For this single variable study, the same low system dispersion/
volume ACQUITY UPLC System was used with all three
columns since use of different LC systems with different
system volumes would affect the final recorded results.
III. COLUMN USE
To ensure the continued high performance of XBridge Glycan
BEH Amide Columns, observe the following guidelines:
a. Sample Preparation
Sample impurities often contribute to column contamination.
Samples should be free of particles before injection into
the system.
In most separations it is preferable to prepare the sample in the
gradient initial composition. However, the labeled glycans are
often insoluble in the high acetonitrile concentrations which
typify HILIC initial conditions. Since small volume injections
are being made, the sample diluents may contain higher
aqueous content (e.g. 50%) than the initial composition.
Note: A mixture of acetonitrile and dimethylformamide is recommended
when analyzing Waters RapiFluor-MS Labeled glycans (see: GlycoWorks
RapiFluor-MS N-Glycan Kit Care and Use Manual p/n: 715004793EN).
If the sample is not dissolved in the mobile-phase or solvent
combinations specified in this manual, ensure that the
sample, solvent, and mobile phases are miscible in order
to avoid sample and/or buffer precipitation. Preparation of
labeled glycans could involve one or two steps of solid-phase
extraction. As a result, protein precipitate has typically been
removed. If not, remove protein particles by centrifugation at
>10,000 rpm for more than 2 minutes.
15 16
ACQUITY Glycan BEH Amide, 130A 1.7 μm
2.1 x 150 mm
0.50 mL/min
ACQUITY Glycan BEH Amide, 130A, 2.5 μm XP
2.1 x 150 mm
0.34 mL/min
7.4
5.0
ACQUITY Glycan BEH Amide, 130A, 3.5 μm
2.1 x 150 mm
0.24 mL/min
10.3
1 2
3
4
5
6
7
89
10
11
12
13
14
8700 psi (Column, Max)
3300 psi (Column, Max)
900 psi (Column, Max)
30.0 min
44.3 min
62.1 min
Figure 2. Comparative scaled separations (based on column particle size differences) using Waters Glycan Performance Test Standard (p/n: 186006349)
on ACQUITY UPLC H-Class System with ACQUITY UPLC Glycan BEH Amide, 130Å, 1.7 µm; XBridge Glycan BEH Amide, 130Å, 2.5 µm XP; and XBridge Glycan
BEH Amide, 130Å, 3.5 µm Columns.
N-Acetylglucosamine
Manose
Galactose
Fucose
Sialic Acid
1
2
3
4
5
6,7
8
9
11
12
13
14
10
6
XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards
[ CARE AND USE MANUAL ]
b. Operating pH Limits
The recommended operating pH range for the XBridge Glycan
BEH Amide, 130Å Column is 3 to 8. A listing of commonly used
buffers and additives is given in Table 2. Additionally, the column
lifetime will vary depending on the operating temperature as well
as the type and concentration of buffer used.
c. Solvents
To maintain maximum column performance, use high quality
chromatography grade solvents. If filtering, Acrodisc® filters
are recommended. Solvents containing suspended particulate
materials can damage the fluidic components of the UPLC
System and will generally clog the inlet distribution frit of the
column. This will result in higher operating pressure and
poor performance.
d. Pressure
The XBridge Glycan BEH Amide, 130Å, 3.5 µm and 2.5 µm XP
Columns will exhibit increased backpressure when operated
in 80–100% aqueous mobile phases. As shown in the gradient
table for Figure 1, the flow rate needs to be lowered when
washing a glycan column. XBridge Glycan BEH Amide, 130Å,
3.5 µm Column can tolerate pressures up to 5000 psi (345 bar
or 34 Mpa) while the maximum operating pressure for the
2.5 µm XP column is approximately 18,000 psi.
Note: Working at the extremes of pressure, pH and/or temperature will result
in shorter column lifetimes.
e. Temperature
Temperatures between 20 °C–90 °C are recommended for
operating XBridge Glycan BEH Amide, 130Å Columns in order
to enhance selectivity, lower solvent viscosity, and increase
mass transfer rates. However, higher temperature will have a
negative effect on lifetime that will vary depending on the pH
and buffer conditions used.
IV. TROUBLESHOOTING
The first step in systematic troubleshooting is comparing
the column, in its current state, to the column when it was
functioning properly. The method suggested in Section II for
measuring plate count is an essential first step. This technique
detects physical changes to the packed bed and chemical
changes in the bonded-phase surface. The functional test with
the 2-AB labeled Dextran Calibration Ladder (p/n: 186006841)
or the Glycan Performance Test Standard (p/n: 186006349)
may reveal more subtle changes in surface chemistry that
affect the application.
There are several common symptoms of change in the column.
An increase in pressure is often associated with lost
performance in the application. The first step in diagnosis is to
ensure that the elevated pressure resides in the column rather
than somewhere else in the system. This is determined by
measuring pressure with and without the column attached to
the instrument. If the system is occluded, the blockage should
be identified and removed. If the pressure increase originates
from the column, it is helpful to know whether the problem was
associated with a single injection or whether it occurred over a
series of injections. If the pressure gradually built up, it is likely
that the column can be cleaned as described below (Section V).
For future stability, it may be useful to incorporate a stronger
regeneration step in the method. If a single sample caused the
pressure increase, it likely reflects particulates or insoluble
components. Cleaning is still an option, but using the more
aggressive methods. The sudden pressure increase suggests
that the user should consider some sample preparation, such
as high speed centrifugation.
Loss of retention can reflect a change in the column surface
chemistry. Before proceeding with diagnostic or corrective
measures, check that the mobile phases have been correctly
prepared and the correct method has been selected. Then
repeat the plate count test and the glycan test standard.
Table 2. Buffer Recommendations for Using ACQUITY Glycan BEH Amide, 130Å Columns from pH 3 to 8
Additive/Buffer
pKa
Buffer Range
(±1 pH unit)
Volatility
Used for
Mass Spec
Comments
Acetic acid
4.76
–
Volatile
Yes
Maximum buffering obtained when used with
ammonium acetate salt. Used in 0.1–1.0% range.
Formic acid
3.75
–
Volatile
Yes
Maximum buffering obtained when used with
ammonium formate salt. Used in 0.1–1.0% range.
Ammonium (acetate)
9.20
8.2–10.2
Volatile
Yes
Up to 100 mM.
Ammonium (formate)
9.20
8.2–10.2
Volatile
Yes
Up to 250 mM.
Triethylamine
(as acetate salt)
9.7–11.7
Volatile
Yes
Used in the 0.1–1.0% range. Volatile only when titrated
with acetic acid (not hydrochloric or phosphoric).
Used as ion pair for DNA analysis at pH 7–9.
7
XBridge Glycan BEH Amide, 130Å, 2.5 µm XP and 3.5 µm Columns and Standards
[ CARE AND USE MANUAL ]
If both the plate count and glycan test show loss of retention,
it is likely that a significant fraction of the bonded phase has
been lost, and the column will require replacement. If the
changes are small and reflected only for some glycans, one of
the cleaning procedures may be effective.
Change in peak shape, resolution, or relative retention of peaks.
Follow the same steps as for loss of retention (Section II).
Carryover and memory effects are defined as the appearance
of the constituents of one sample in the next gradient analysis.
First determine whether the column or the system is the
source of carryover. Define a gradient method that includes an
“internal gradient”. That is, the analytical gradient is repeated
within a single method. If the glycan peaks appear in both
gradients, at the same time after start, the carryover came
from the column in what is often described as a “memory
effect”. If the glycan peaks only appear when an injection is
made, they likely originated from adsorption to some system
component. In that case follow the instrument manufacturer’s
recommendations. Memory effects as a source of carryover
may be reduced or eliminated in several ways. First, raising
the temperature of the separation reduces the possibility of
non-specific adsorption. Second, memory effects may be
more pronounced with steep gradients. Keep the gradient
slope at 1% per column volume or less. Third, memory effects
may be exacerbated by high flow rates. Reduce the flow rate
by one half while doubling the gradient time to maintain a
constant slope. Finally, apparent memory effects may actually
reflect the solubility of the sample in the mobile phase.
Reducing the amount injected may eliminate the effect.
Note: Useful general information on column troubleshooting problems may
be found in HPLC Columns Theory, Technology and Practice, U.D. Neue,
Wiley-VCH, 1997 (p/n: WAT038216), the Waters HPLC Troubleshooting Guide
(Literature code # 720000181EN), or visit www.waters.com.
V. COLUMN CLEANING, REGENERATION,
AND STORAGE
a. Cleaning and Regeneration
Changes in peak shape, peak splitting, shoulders on the peak,
shifts in retention, change in resolution, carryover, ghost peaks,
or increasing backpressure may indicate contamination of the
column. Choose a cleaning option that may be expected to
dissolve the suspected contaminant.
1. All cleaning procedures will be more effective at higher
temperatures. It is reasonable to conduct cleaning at 70 °C.
2. It may be useful to conduct cleaning procedures at one
half the flow rate typically used with that column. In this
way, the possibility of high pressure events is reduced.
3. The first and simplest cleaning procedure is to run a
series of gradients from 0–100% water. Be sure to reduce
the flow rate for gradients with higher than 75% aqueous
content. Columns of 150 mm length should be operated at
250 µL per minute or less during washes. The gradients
can be as short as 5 column volumes and 3–5 repetitions
may be effective.
4. Regeneration steps and flushing procedures using
100% aqueous mobile phase can help to maintain the
optimal peak shape and selectivity of a HILIC separation.
Additionally, an analyst can perform gradients with
mobile phases containing 0.1% TFA as a means to
maintain or recover the performance of a Glycoprotein
BEH Amide column. TFA can be effective in cleaning
a column’s stationary phase by both neutralizing and
ion pairing contaminants that in their ionic form might
otherwise strongly adsorb to a HILIC stationary phase.
5. Several different cleaning solutions may be injected to
strip strongly adsorbed material or particulates from
the column. Make the largest injection possible with
the system configuration. With such strong cleaning
solutions, it is best to disconnect the detector from the
column and to direct the flow to waste.
6. Flow reversal or backflushing is often suggested as part
of a cleaning procedure. This should be reserved as a last
resort. It may further damage the column or provide a
short-lived improvement in performance.
b. Storage
For periods longer than four days at room temperature, store
the column in 100% acetonitrile. Immediately after use with
elevated temperatures and/or at pH extremes, store in 100%
acetonitrile for the best column lifetime. Do not store columns
in highly aqueous (<50% organic) mobile phases, as this may
promote bacterial growth. If the mobile phase contained a
buffer salt, flush the column with 10 column volumes of HPLC
grade water (see Table 1 for common column volumes) and
replace with 100% acetonitrile for storage. Failure to perform
this intermediate step could result in precipitation of the
buffer salt in the column or system when 100% acetonitrile is
introduced. Completely seal the column to avoid evaporation
and drying out the bed.
Note: If a column has been run with a mobile phase that contains formate
(e.g., ammonium formate, formic acid, etc.) and is then flushed with 100%
acetonitrile, slightly longer equilibration times may be necessary when the
column is re-installed and run again with a formate-containing mobile phase.
[ CARE AND USE MANUAL ]
Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com
[ CARE AND USE MANUAL ]
Waters, The Science of What’s Possible, ACQUITY, UPLC, and XBridge are registered trademarks
of Waters Corporation. RapiFluor-MS and BEH Technology are trademarks of Waters Corporation.
All other trademarks are the property of their respective owners.
©2017 Waters Corporation. Produced in the U.S.A. July 2017 Rev H 720004882EN IH-PDF
VII. ORDERING INFORMATION (Partial listing. For more information, visit www.waters.com)
Product Description
Pore Size
Particle Size
Dimension
P/N
XBridge Glycan BEH Amide
130Å
2.5 µm
3.0 x 30 mm XP
186008038
XBridge Glycan BEH Amide
130Å
2.5 µm
3.0 x 75 mm XP
186008039
XBridge Glycan BEH Amide
130Å
2.5 µm
3.0 x 150 mm XP
186008040
XBridge Glycan BEH Amide
130Å
2.5 µm
VanGuard Pre-Column
186007262
XBridge Glycan BEH Amide
130Å
2.5 µm
2.1 x 50 mm XP
186007263
XBridge Glycan BEH Amide
130Å
2.5 µm
2.1 x 100 mm XP
186007264
XBridge Glycan BEH Amide
130Å
2.5 µm
2.1 x 150 mm XP
186007265
XBridge Glycan BEH Amide
130Å
2.5 µm
2.1 x 150 XP MVK
186007266
XBridge Glycan BEH Amide
130Å
2.5 µm
4.6 x 20 mm Guard, 2/pkg
186007267
XBridge Glycan BEH Amide
130Å
2.5 µm
4.6 x 50 mm XP
186007268
XBridge Glycan BEH Amide
130Å
2.5 µm
4.6 x 100 mm XP
186007269
XBridge Glycan BEH Amide
130Å
2.5 µm
4.6 x 150 mm XP
186007270
XBridge Glycan BEH Amide
130Å
2.5 µm
4.6 x 150 XP MVK
186007271
XBridge Glycan BEH Amide
130Å
3.5 µm
4.6 x 20 mm Guard, 2/pkg
186007272
XBridge Glycan BEH Amide
130Å
3.5 µm
4.6 x 50 mm
186007273
XBridge Glycan BEH Amide
130Å
3.5 µm
4.6 x 100 mm
186007274
XBridge Glycan BEH Amide
130Å
3.5 µm
4.6 x 150 mm
186007275
XBridge Glycan BEH Amide
130Å
3.5 µm
4.6 x 250 mm
186007276
XBridge Glycan BEH Amide
130Å
3.5 µm
4.6 x 150 MVK
186007277
XBridge Glycan BEH Amide
130Å
3.5 µm
2.1 x 10 mm Guard, 2/pkg
186007505
XBridge Glycan BEH Amide
130Å
3.5 µm
2.1 x 50 mm
186007502
XBridge Glycan BEH Amide
130Å
3.5 µm
2.1 x 100 mm
186007503
XBridge Glycan BEH Amide
130Å
3.5 µm
2.1 x 150 mm
186007504
Glycan Performance Test Standard
186006349
VI. CAUTIONARY NOTE
Depending on users application, these products may be
classified as hazardous following their use and as such are
intended to be used by professional laboratory personnel
trained in the competent handling of such materials.
Responsibility for the safe use and disposal of products rests
entirely with the purchaser and user. The Safety Data Sheet
(SDS) for this product is available at www.waters.com.
Similar PDF
Key words
Key words
Key words