Fast Separation of Monoclonal Antibody and Dimer by SEC with Agilent Bio SEC

Applications | 2011 | Agilent TechnologiesInstrumentation
Consumables, LC columns, GPC/SEC
Industries
Pharma & Biopharma
Manufacturer
Agilent Technologies
Key words
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Mobile Phase Optimization in SEC Method Development
2018|Agilent Technologies|Applications
Application NotePharma & BiopharmaMobile Phase Optimization in SECMethod DevelopmentAuthorRichard HurteauAgilent Technologies, Inc.,Wilmington, DE, USAAbstractAggregation of monoclonal antibody (mAb) products has an adverse effect ontheir efficacy as therapeutic agents. Size exclusion chromatography (SEC) is ahighly effective method for the detection of...
Key words
mau, maumin, minigg, iggkda, kdaphosphate, phosphateinsulin, insulinaggregation, aggregationbuffer, bufferadc, adccytochrome, cytochromecolumns, columnsproteins, proteinsnriu, nriutherapeutic, therapeuticpeak
Helpful ‘Hints’ for Protein SEC Success
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Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201 Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
Key words
hints, hintshelpful, helpfulsec, secsuccess, successprotein, proteinpore, poreadvancebio, advancebiosize, sizebuffer, bufferexclusion, exclusionmobile, mobilephase, phasecolumn, columndimer, dimervolume
Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
Key words
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[ APPLICATION NOTEBOOK ]SIZE-EXCLUSIONCHROMATOGRAPHY (SEC)OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ]IntroductionGel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtrationchromatography (GFC) are commonly used terms to describe a liquid chromatographic techniquethat separates compounds based on their relative different...
Key words
sec, secexclusion, exclusionsize, sizeuplc, uplcacquity, acquitychromatography, chromatographyprotein, proteinaggregates, aggregateshplc, hplcdispersion, dispersionmonomer, monomercolumn, columnantibody, antibodyigg, igghmws