Fast Separation of Monoclonal Antibody and Dimer by SEC with Agilent Bio SECApplications | 2011 | Agilent Technologies Instrumentation Consumables, LC columns, GPC/SECIndustriesPharma & BiopharmaManufacturerAgilent TechnologiesKey wordsdimer, eluent, salted, monomer, monoclonal, antibody, salt, competitor, percentage, agilent, flow, rate, column, efficiency, ratio, exclusion, mab, resolution, sec, revealing, backpressures, tim, detection, usa, suit, injection, aggregation, fast, rice, therapeutics, eluents, analysis, conditions, biopharma, without, concern, separation, shorter, helps, maintaining, sample, bio, consequential, incidental, dependent, become, liable, chromatography, shall, rates
Mobile Phase Optimization in SEC Method Development
Application NotePharma & BiopharmaMobile Phase Optimization in SECMethod DevelopmentAuthorRichard HurteauAgilent Technologies, Inc.,Wilmington, DE, USAAbstractAggregation of monoclonal antibody (mAb) products has an adverse effect ontheir efficacy as therapeutic agents. Size exclusion chromatography (SEC) is ahighly effective method for the detection of...
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Helpful ‘Hints’ for Protein SEC Success
Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
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Agilent Biocolumns - Aggregate/Fragment Analysis - Application Compendium
Agilent BiocolumnsAggregate/Fragment AnalysisApplication CompendiumContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
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SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF PEPTIDES, PROTEINS, AND AAVs
[ APPLICATION NOTEBOOK ]SIZE-EXCLUSIONCHROMATOGRAPHY (SEC)OF PEPTIDES, PROTEINS, AND AAVs[ SIZE-EXCLUSION CHROMATOGRAPHY ][ SIZE-EXCLUSION CHROMATOGRAPHY ]IntroductionGel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtrationchromatography (GFC) are commonly used terms to describe a liquid chromatographic techniquethat separates compounds based on their relative different...
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