Fast Separation of Monoclonal Antibody and Dimer by SEC with Agilent Bio SEC | LabRulez LCMS
 

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Mobile Phase Optimization in SEC Method Development
2018|Agilent Technologies|Applications
Application NotePharma & BiopharmaMobile Phase Optimization in SECMethod DevelopmentAuthorRichard HurteauAgilent Technologies, Inc.,Wilmington, DE, USAAbstractAggregation of monoclonal antibody (mAb) products has an adverse effect ontheir efficacy as therapeutic agents. Size exclusion chromatography (SEC) is ahighly effective method for the detection of...
Key words
mau, maumin, minigg, iggphosphate, phosphatekda, kdaaggregation, aggregationinsulin, insulinbuffer, bufferadc, adccytochrome, cytochromeproteins, proteinscolumns, columnsnriu, nriutherapeutic, therapeuticpeak
Helpful ‘Hints’ for Protein SEC Success
2021|Agilent Technologies|Presentations
Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201 Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
Key words
hints, hintshelpful, helpfulsec, secsuccess, successprotein, proteinpore, poresize, sizeadvancebio, advancebiobuffer, bufferexclusion, exclusionmobile, mobilephase, phasedimer, dimercolumn, columnvolume
Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
Key words
return, returnsection, sectioncontents, contentsmau, mausec, secinsulin, insulinmin, minsize, sizeprotein, proteinmonomer, monomeradvancebio, advancebiotime, timeexclusion, exclusionaggregates, aggregatesaggregate
[ APPLICATION NOTEBOOK ]SIZE-EXCLUSIONCHROMATOGRAPHY (SEC)OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ]IntroductionGel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtrationchromatography (GFC) are commonly used terms to describe a liquid chromatographic techniquethat separates compounds based on their relative different...
Key words
sec, secexclusion, exclusionsize, sizeuplc, uplcacquity, acquitychromatography, chromatographyprotein, proteinaggregates, aggregateshplc, hplcdispersion, dispersionmonomer, monomerantibody, antibodyigg, iggcolumn, columnbio
 

Similar PDF

Toggle
Mobile Phase Optimization in SEC Method Development
2018|Agilent Technologies|Applications
Application NotePharma & BiopharmaMobile Phase Optimization in SECMethod DevelopmentAuthorRichard HurteauAgilent Technologies, Inc.,Wilmington, DE, USAAbstractAggregation of monoclonal antibody (mAb) products has an adverse effect ontheir efficacy as therapeutic agents. Size exclusion chromatography (SEC) is ahighly effective method for the detection of...
Key words
mau, maumin, minigg, iggphosphate, phosphatekda, kdaaggregation, aggregationinsulin, insulinbuffer, bufferadc, adccytochrome, cytochromeproteins, proteinscolumns, columnsnriu, nriutherapeutic, therapeuticpeak
Helpful ‘Hints’ for Protein SEC Success
2021|Agilent Technologies|Presentations
Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201 Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
Key words
hints, hintshelpful, helpfulsec, secsuccess, successprotein, proteinpore, poresize, sizeadvancebio, advancebiobuffer, bufferexclusion, exclusionmobile, mobilephase, phasedimer, dimercolumn, columnvolume
Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
Key words
return, returnsection, sectioncontents, contentsmau, mausec, secinsulin, insulinmin, minsize, sizeprotein, proteinmonomer, monomeradvancebio, advancebiotime, timeexclusion, exclusionaggregates, aggregatesaggregate
[ APPLICATION NOTEBOOK ]SIZE-EXCLUSIONCHROMATOGRAPHY (SEC)OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ]IntroductionGel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtrationchromatography (GFC) are commonly used terms to describe a liquid chromatographic techniquethat separates compounds based on their relative different...
Key words
sec, secexclusion, exclusionsize, sizeuplc, uplcacquity, acquitychromatography, chromatographyprotein, proteinaggregates, aggregateshplc, hplcdispersion, dispersionmonomer, monomerantibody, antibodyigg, iggcolumn, columnbio
 

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