Multiple Detector Approaches to Protein Aggregation by SEC | LabRulez LCMS
 

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Agilent-NISTmAbAggregate andFragment AnalysisAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information. A certificate of...
Key words
page, pagecontents, contentsmobile, mobileback, backsec, secbuffer, bufferexclusion, exclusionmau, maunacl, naclmonomer, monomerhwm, hwmdimer, dimerphase, phasesize, sizeresolution
Size Exclusion Chromatographyin the Presence of an AnionicSurfactantIntact Protein ProfilingApplication NoteAuthorAbstractAndy CoffeySodium dodecyl sulfate (SDS, or SLS) is a well known anionic detergent,Agilent Technologies, Incfrequently used to denature proteins. It is commonly used in polyacrylamide gelelectrophoresis (SDS-PAGE), where a remarkably...
Key words
sds, sdsbsa, bsaaggregates, aggregatesmonomer, monomerdimer, dimerscattering, scatteringmolecular, molecularlight, lightprotein, proteinweight, weighthydrodynamic, hydrodynamichigher, higherorder, orderdls, dlsoligomers
Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
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return, returnsection, sectioncontents, contentsmau, mausec, secinsulin, insulinmin, minsize, sizeprotein, proteinmonomer, monomeradvancebio, advancebiotime, timeexclusion, exclusionaggregates, aggregatesaggregate
Agilent “How to” Guide forSIZE EXCLUSION CHROMATOGRAPHYFOR BIOMOLECULE ANALYSIS A GUIDE TO SUCCESSFUL SECThe chromatographic separation of biomolecules based on their size in solution is knownas size exclusion chromatography (SEC). Unlike other modes of chromatography, it relieson the absence of any...
Key words
size, sizemau, mausec, secpore, porecolumn, columnproteins, proteinsmolecular, molecularweight, weightexclusion, exclusionpolypropylene, polypropylenecolumns, columnsshould, shouldsmaller, smalleragilent, agilentsample
 

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Agilent-NISTmAbAggregate andFragment AnalysisAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information. A certificate of...
Key words
page, pagecontents, contentsmobile, mobileback, backsec, secbuffer, bufferexclusion, exclusionmau, maunacl, naclmonomer, monomerhwm, hwmdimer, dimerphase, phasesize, sizeresolution
Size Exclusion Chromatographyin the Presence of an AnionicSurfactantIntact Protein ProfilingApplication NoteAuthorAbstractAndy CoffeySodium dodecyl sulfate (SDS, or SLS) is a well known anionic detergent,Agilent Technologies, Incfrequently used to denature proteins. It is commonly used in polyacrylamide gelelectrophoresis (SDS-PAGE), where a remarkably...
Key words
sds, sdsbsa, bsaaggregates, aggregatesmonomer, monomerdimer, dimerscattering, scatteringmolecular, molecularlight, lightprotein, proteinweight, weighthydrodynamic, hydrodynamichigher, higherorder, orderdls, dlsoligomers
Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
Key words
return, returnsection, sectioncontents, contentsmau, mausec, secinsulin, insulinmin, minsize, sizeprotein, proteinmonomer, monomeradvancebio, advancebiotime, timeexclusion, exclusionaggregates, aggregatesaggregate
Agilent “How to” Guide forSIZE EXCLUSION CHROMATOGRAPHYFOR BIOMOLECULE ANALYSIS A GUIDE TO SUCCESSFUL SECThe chromatographic separation of biomolecules based on their size in solution is knownas size exclusion chromatography (SEC). Unlike other modes of chromatography, it relieson the absence of any...
Key words
size, sizemau, mausec, secpore, porecolumn, columnproteins, proteinsmolecular, molecularweight, weightexclusion, exclusionpolypropylene, polypropylenecolumns, columnsshould, shouldsmaller, smalleragilent, agilentsample
 

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