Multiple Detector Approaches to Protein Aggregation by SECApplications | 2017 | Agilent TechnologiesInstrumentationGPC/SECIndustriesPharma & BiopharmaManufacturerAgilent TechnologiesKey wordsaggregates, aggregation, dimer, monomer, globulin, sonicated, higher, sonication, protein, response, bovine, sec, dimerization, importance, size, detector, exclusion, agglomerates, affect, can, noticeably, responsive, shear, coffey, min, effect, paramount, investigates, strengths, andrew, insoluble, undesirable, temperature, treat, serious, during, sample, template, understood, stress, itself, mol, biotherapeutic, scattering, recognized, consistently, suite, aid, biopharma, minor
Clearing the Confusion: GPC, SEC, GFC –What , When, Why, and How?
Clearing the Confusion:GPC, SEC, GFC –What , When, Why, andHow?Jean LaneApplications EngineerLC Columns & ConsumablesTechnical SupportJanuary 19, 2017What we will cover……What- Clarification of the termsNomenclatureHow is the separation accomplished – mode of separationKey definitionsWhen & WhySimilarities & Differencesanalysis goals -...
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Aggregate and Fragment Analysis - Agilent BioHPLC Columns Application Compendium
Agilent-NISTmAbAggregate andFragment AnalysisAgilent BioHPLC ColumnsApplication CompendiumContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information. A certificate of...
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Size Exclusion Chromatography in the Presence of an Anionic Surfactant
Size Exclusion Chromatographyin the Presence of an AnionicSurfactantIntact Protein ProfilingApplication NoteAuthorAbstractAndy CoffeySodium dodecyl sulfate (SDS, or SLS) is a well known anionic detergent,Agilent Technologies, Incfrequently used to denature proteins. It is commonly used in polyacrylamide gelelectrophoresis (SDS-PAGE), where a remarkably...
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Helpful ‘Hints’ for Protein SEC Success
Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
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