Characterization of mAb Aggregation | LabRulez LCMS_EN
 

Similar PDF

Toggle
Application NoteBiologicsHigh-Throughput Fluorescence-BasedmAbs Aggregation Analysis WorkflowEfficiency gains when using fluorescence analysisto complement size exclusion chromatographyAuthorsIntroductionBrian LiauAgilent Technologies, Inc.Modern biologics development relies on the screening of 100s - 1000s ofmonoclonal antibody (mAb) variants to identify lead development candidates withoptimal properties such...
Key words
aggregation, aggregationfluorescence, fluorescencepepbops, pepbopsmabs, mabsestimates, estimatesrituximab, rituximabskim, skimsypro, syprotrastuzumab, trastuzumabmab, mabans, ansreader, readerantibody, antibodyreject, rejectbuffer
High-throughput and Sensitive SizeExclusion Chromatography (SEC) ofBiologics Using Agilent AdvanceBioSEC ColumnsAgilent AdvanceBio SEC 300 Å, 2.7 µm columnsApplication noteBio-PharmaceuticalAuthorAbstractM. Sundaram PalaniswamyMonoclonal antibody (mAb) aggregation can arise due to multiple mechanismsAgilent Technologies Inc.during cell culture, harvest, purification, storage, and filling. Size...
Key words
adc, adcherceptin, herceptinmau, mauadvancebio, advancebiosec, secmonomer, monomeraggregate, aggregatemab, mabaggregates, aggregatesoverlay, overlayreplicates, replicatesstressed, stresseddegraded, degradedpreservative, preservativecolumns
The Analysis of Multimeric Monoclonal Antibody Aggregatesby Size-Exclusion UPLCStephan Koza, Matthew Lauber, and Kenneth J. FountainWaters Corporation, Milford, MA, USAA P P L I C AT I O N B E N E F I T S■■ACQUITY UPLC SEC Technology...
Key words
multimeric, multimericmultimers, multimersdimer, dimeraggregates, aggregatesuplc, uplctrimer, trimerexclusion, exclusionmonoclonal, monoclonalmonomer, monomerdimeric, dimericsize, sizemab, mabantibody, antibodyaggregate, aggregateforms
Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
Key words
return, returnsection, sectioncontents, contentsmau, mausec, secinsulin, insulinmin, minsize, sizeprotein, proteinmonomer, monomeradvancebio, advancebiotime, timeexclusion, exclusionaggregates, aggregatesaggregate
 

Similar PDF

Toggle
Application NoteBiologicsHigh-Throughput Fluorescence-BasedmAbs Aggregation Analysis WorkflowEfficiency gains when using fluorescence analysisto complement size exclusion chromatographyAuthorsIntroductionBrian LiauAgilent Technologies, Inc.Modern biologics development relies on the screening of 100s - 1000s ofmonoclonal antibody (mAb) variants to identify lead development candidates withoptimal properties such...
Key words
aggregation, aggregationfluorescence, fluorescencepepbops, pepbopsmabs, mabsestimates, estimatesrituximab, rituximabskim, skimsypro, syprotrastuzumab, trastuzumabmab, mabans, ansreader, readerantibody, antibodyreject, rejectbuffer
High-throughput and Sensitive SizeExclusion Chromatography (SEC) ofBiologics Using Agilent AdvanceBioSEC ColumnsAgilent AdvanceBio SEC 300 Å, 2.7 µm columnsApplication noteBio-PharmaceuticalAuthorAbstractM. Sundaram PalaniswamyMonoclonal antibody (mAb) aggregation can arise due to multiple mechanismsAgilent Technologies Inc.during cell culture, harvest, purification, storage, and filling. Size...
Key words
adc, adcherceptin, herceptinmau, mauadvancebio, advancebiosec, secmonomer, monomeraggregate, aggregatemab, mabaggregates, aggregatesoverlay, overlayreplicates, replicatesstressed, stresseddegraded, degradedpreservative, preservativecolumns
The Analysis of Multimeric Monoclonal Antibody Aggregatesby Size-Exclusion UPLCStephan Koza, Matthew Lauber, and Kenneth J. FountainWaters Corporation, Milford, MA, USAA P P L I C AT I O N B E N E F I T S■■ACQUITY UPLC SEC Technology...
Key words
multimeric, multimericmultimers, multimersdimer, dimeraggregates, aggregatesuplc, uplctrimer, trimerexclusion, exclusionmonoclonal, monoclonalmonomer, monomerdimeric, dimericsize, sizemab, mabantibody, antibodyaggregate, aggregateforms
Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
Key words
return, returnsection, sectioncontents, contentsmau, mausec, secinsulin, insulinmin, minsize, sizeprotein, proteinmonomer, monomeradvancebio, advancebiotime, timeexclusion, exclusionaggregates, aggregatesaggregate
 

Related content

Overcoming the challenges of liquid chromatography method transfer: A CDMO perspective

Technical notes
| 2022 | Thermo Fischer Scientific
Instrumentation
HPLC
Manufacturer
Thermo Fischer Scientific
Industries
---

Method for the determination of 431 Residual Pesticides in Honey using LCMS-8050 and GCMS-TQ8040 NX

Applications
| 2022 | Shimadzu
Instrumentation
GC/MSD, GC/MS/MS, GC/QQQ, LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Shimadzu
Industries
Food & Agriculture

ASMS: IMPROVEMENTS IN SENSITIVITY, QUANTITATIVE LINEARITY, AND ROBUSTNESS USING A MODIFIED BENCH-TOP QUADRUPOLE ORTHOGONAL TIME-OF-FLIGHT MASS SPECTROMETER (Q-TOF MS)

Posters
| 2022 | Waters
Instrumentation
LC/TOF, LC/HRMS, LC/MS, LC/MS/MS
Manufacturer
Waters
Industries
---

Determination of Per and Polyfluoroalkyl Substances in Soils Using Carbon S SPE by LC/MS/MS

Applications
| 2022 | Agilent Technologies
Instrumentation
Sample Preparation, Consumables, LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Agilent Technologies
Industries
Environmental

ASTM D4327-03 Compliant Analysis of Anions in Wastewater

Applications
| 2022 | Shimadzu
Instrumentation
Ion chromatography
Manufacturer
Shimadzu
Industries
Environmental
 

Related content

Overcoming the challenges of liquid chromatography method transfer: A CDMO perspective

Technical notes
| 2022 | Thermo Fischer Scientific
Instrumentation
HPLC
Manufacturer
Thermo Fischer Scientific
Industries
---

Method for the determination of 431 Residual Pesticides in Honey using LCMS-8050 and GCMS-TQ8040 NX

Applications
| 2022 | Shimadzu
Instrumentation
GC/MSD, GC/MS/MS, GC/QQQ, LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Shimadzu
Industries
Food & Agriculture

ASMS: IMPROVEMENTS IN SENSITIVITY, QUANTITATIVE LINEARITY, AND ROBUSTNESS USING A MODIFIED BENCH-TOP QUADRUPOLE ORTHOGONAL TIME-OF-FLIGHT MASS SPECTROMETER (Q-TOF MS)

Posters
| 2022 | Waters
Instrumentation
LC/TOF, LC/HRMS, LC/MS, LC/MS/MS
Manufacturer
Waters
Industries
---

Determination of Per and Polyfluoroalkyl Substances in Soils Using Carbon S SPE by LC/MS/MS

Applications
| 2022 | Agilent Technologies
Instrumentation
Sample Preparation, Consumables, LC/MS, LC/MS/MS, LC/QQQ
Manufacturer
Agilent Technologies
Industries
Environmental

ASTM D4327-03 Compliant Analysis of Anions in Wastewater

Applications
| 2022 | Shimadzu
Instrumentation
Ion chromatography
Manufacturer
Shimadzu
Industries
Environmental
Other projects
Follow us
More information
WebinarsAbout usContact usTerms of use

LabRulez s.r.o., all rights reserved.