Lipid Nanoparticle Compositional Analysis Using Charged Surface Hybrid Phenyl-Hexyl Separation With Evaporative Light Scattering Detection
Applications | 2021 | WatersInstrumentation
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Analysis of Lipid Nanoparticle Composition
Application NoteBiopharmaAnalysis of LipidNanoparticle CompositionAuthorSonja SchneiderAgilent Technologies, Inc.0DLin-MC3-DMADMG-PEG 2000DSPC×1024.754.504.254.003.753.503.253.002.752.502.252.001.751.501.251.000.750.50CholesterolResponse (LSU)Quaternary method development for highestresolution with evaporative light scattering detection0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5Retention time (min)AbstractLipid nanoparticles...
dspc, dspclipid, lipidcholesterol, cholesterollsu, lsulnp, lnpchannel, channelmeoh, meohcomponents, componentsfour, fouracn, acnresponse, responsequaternary, quaternaryretention, retentionrna, rnagradient
Lipid Nanoparticle Analysis: Leveraging MS to Reduce Risk
Application NoteLipid Nanoparticle Analysis: Leveraging MSto Reduce RiskDuanduan Han, Kellen DeLaney, Bonnie A. Alden, Robert E. Birdsall, Ying Qing YuWaters CorporationAbstractLipid nanoparticles (LNPs) represent an elegant solution in the delivery of gene-based therapeutics that can bechallenging to execute. As a...
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Characterizing and Monitoring Impurities in Lipid Nanoparticle Components Using the BioAccord™ LC-MS System with waters_connect™ Software
Application NoteCharacterizing and Monitoring Impurities inLipid Nanoparticle Components Using theBioAccord™ LC-MS System withwaters_connect™ SoftwareKellen DeLaney, Duanduan Han, Robert E. Birdsall, Ying Qing YuWaters CorporationAbstractLipid nanoparticles (LNP) have been used with high efficacy as delivery vehicles for nucleic-acid basedtherapeutics and vaccines....
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ASMS: Lipid Nanoparticle Impurity Monitoring Using Single Quadrupole Mass Detection for Regulated Environments
Lipid Nanoparticle Impurity Monitoring Using Single Quadrupole Mass Detectionfor Regulated EnvironmentsAuthors: Duanduan Han, Kellen DeLaney, Robert E. Birdsall, Ying Qing YuAffiliation: Waters Corporation, Milford, MAResultsIntroductionLipid NanoparticlesUV3AU2Cholesterol• Important for endocytosisSM102 (ionizable lipid)DOTMA (ionizable lipid)CholesterolDMG-PEG2000 (PEGylated lipid)DSPC (phospholipid)3ELSDNanogramLSUUV activenon-UV activenon-volatilesemi-volatile1510%4PEGylated lipid (DMG-PEG2000)•Stabilizes...
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