Lipid Nanoparticle Compositional Analysis Using Charged Surface Hybrid Phenyl-Hexyl Separation With Evaporative Light Scattering Detection

Applications | 2021 | WatersInstrumentation
HPLC
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Lipidomics
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Waters
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Analysis of Lipid Nanoparticle Composition
2022|Agilent Technologies|Applications
Application NoteBiopharmaAnalysis of LipidNanoparticle CompositionAuthorSonja SchneiderAgilent Technologies, Inc.0DLin-MC3-DMADMG-PEG 2000DSPC×1024.754.504.254.003.753.503.253.002.752.502.252.001.751.501.251.000.750.50CholesterolResponse (LSU)Quaternary method development for highestresolution with evaporative light scattering detection0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5Retention time (min)AbstractLipid nanoparticles...
Key words
dspc, dspclipid, lipidcholesterol, cholesterollsu, lsulnp, lnpchannel, channelmeoh, meohcomponents, componentsfour, fouracn, acnresponse, responsequaternary, quaternaryretention, retentionrna, rnagradient
Application NoteLipid Nanoparticle Analysis: Leveraging MSto Reduce RiskDuanduan Han, Kellen DeLaney, Bonnie A. Alden, Robert E. Birdsall, Ying Qing YuWaters CorporationAbstractLipid nanoparticles (LNPs) represent an elegant solution in the delivery of gene-based therapeutics that can bechallenging to execute. As a...
Key words
lnp, lnpqda, qdaempower, empowerspectral, spectrallipid, lipidgene, geneimpurity, impuritydetector, detectormass, massdual, dualcomplementary, complementaryacquity, acquityrepresent, representdata, dataworkflow
Application NoteCharacterizing and Monitoring Impurities inLipid Nanoparticle Components Using theBioAccord™ LC-MS System withwaters_connect™ SoftwareKellen DeLaney, Duanduan Han, Robert E. Birdsall, Ying Qing YuWaters CorporationAbstractLipid nanoparticles (LNP) have been used with high efficacy as delivery vehicles for nucleic-acid basedtherapeutics and vaccines....
Key words
bioaccord, bioaccordlipid, lipidnanoparticle, nanoparticlecharacterizing, characterizingimpurities, impuritiesmonitoring, monitoringcomponents, componentssystem, systemlnp, lnpusing, usingfragment, fragmentvehicles, vehiclesmatching, matchingimpurity, impuritynanoparticles
Lipid Nanoparticle Impurity Monitoring Using Single Quadrupole Mass Detectionfor Regulated EnvironmentsAuthors: Duanduan Han, Kellen DeLaney, Robert E. Birdsall, Ying Qing YuAffiliation: Waters Corporation, Milford, MAResultsIntroductionLipid NanoparticlesUV3AU2Cholesterol• Important for endocytosisSM102 (ionizable lipid)DOTMA (ionizable lipid)CholesterolDMG-PEG2000 (PEGylated lipid)DSPC (phospholipid)3ELSDNanogramLSUUV activenon-UV activenon-volatilesemi-volatile1510%4PEGylated lipid (DMG-PEG2000)•Stabilizes...
Key words
elsd, elsddspc, dspcdesaturation, desaturationoxidation, oxidationsaturation, saturationdata, datalipid, lipidimpurity, impuritycompositional, compositionalcholesterol, cholesterolqda, qdamass, massassignment, assignmentcomplementary, complementaryvolatile