ASMS 2021: Neutral pH Reversed Phase LC-MS Methodology for the Recovery and Characterization of Highly Acidic Peptides of EnbrelPosters | 2021 | WatersInstrumentationLC/TOF, LC/HRMS, LC/MS, LC/MS/MSIndustriesProteomics ManufacturerWatersKey wordsneutral, acidic, phase, chelation, mobile, glycopeptides, reversed, conventional, unrecoverable, methodology, mitigating, peptides, previously, enbrel, phosphopeptides, sialylated, fluidics, nonspecific, highly, rplc, negatively, infusion, employ, tryptic, reactions, surfaces, digest, experience, coverage, mapping, characterize, formic, mab, successful, nist, maintaining, path, sequence, peptide, expected, loss, detect, characterization, integrated, post, typical, significant, complete, detected, some
Agilent BioHPLC Columns - Characterization of NIST Monoclonal Antibody Critical Quality Attributes - Application Compendium
Agilent BioHPLC ColumnsCharacterization of NIST MonoclonalAntibody Critical Quality AttributesApplication CompendiumContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass...
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HILIC Glycopeptide Mapping with a Wide-Pore Amide Stationary Phase
HILIC Glycopeptide Mapping with a Wide-Pore Amide Stationary PhaseMatthew A. Lauber and Stephan M. KozaWaters Corporation, Milford, MA, USAA P P L I C AT I O N B E N E F I T S■■Orthogonal selectivity to conventionalreversed phase...
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Improved Recovery and Peak Shape of Sialylated O-glycopeptides of Erythropoietin (EPO) with ACQUITY Premier CSH Column
Application NoteImproved Recovery and Peak Shape ofSialylated O-glycopeptides ofErythropoietin (EPO) with ACQUITYPremier CSH ColumnSamantha Ippoliti, Ying Qing Yu, Weibin ChenWaters CorporationThis is an Application Brief and does not contain a detailed Experimental section.AbstractPeptide mapping via LC-MS is a powerful characterization...
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How to Determine Glycan Profiles of Biopharmaceuticals from Peptide Mapping Data
How to Determine Glycan Profiles ofBiopharmaceuticals from Peptide Mapping DataA new and sensitive approach combining BioPharma Compass, PASEF and VIP-HESIAbstractGlycosylation is a commoncritical quality attribute (CQA)of therapeutic proteins andneeds to be characterized duringproduct development. Typically,this analysis is conducted afterenzymatic release...
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