ASMS 2021: Rapid Analysis of Synthetic mRNA Cap Structure using Ion Pairing Reversed Phase Liquid Chromatography coupled to Mass SpectrometryPosters | 2021 | WatersInstrumentationHPLC, LC/MSIndustriesPharma & BiopharmaManufacturerWatersKey wordssynthetic, oligonucleotides, probe, rnase, mrna, uhplc, fifth, surface, capping, coupled, heavily, rna, marked, pairing, dispersion, detecting, hybrid, when, influence, applicable, improvements, using, representative, sequence, impurities, choice, down, inert, conventional, analyzing, related, system, injections, can, sensitive, analyses, rapid, achieved, containing, less, column, from, recovery, first, highly, obtained, performed, same, than, quality
Similar PDF
Application NoteImproved Chromatographic Analysis of Oligonucleotides with ACQUITY PREMIER Oligonucleotide BEH C18 Columns
2020|Waters|Applications
Application NoteImproved Chromatographic Analysis ofOligonucleotides with ACQUITY PREMIEROligonucleotide BEH C18 ColumnsMartin Gilar, Zhimin Li, Jason MacLeanWaters Corporation AbstractThis application note illustrates sources of oligonucleotide loss due to adsorption on metalliccolumn hardware (frits). Solutions, columns, and best practices for improved oligonucleotideanalysis are...
Key words
oligonucleotide, oligonucleotideoligonucleotides, oligonucleotidesmiser, misersurfaces, surfacesmetallic, metallicfrit, fritadsorption, adsorptionuplc, uplcacquity, acquitypremier, premierstainless, stainlesssteel, steelhps, hpsmaxpeak, maxpeakoligodeoxythymidine
Application NoteBiopharmaRapid Analysis of mRNA 5' Cappingwith High Resolution LC/MSAuthorBrian LiauAgilent Technologies, Inc.IntroductionIndustrial-scale production of mRNA has received growing attention due to theongoing SARS-CoV-2 pandemic. mRNA vaccines have proven to be one of the mosteffective tools against the virus and...
Key words
mrna, mrnacapping, cappingchimeric, chimericrnase, rnasedeconvoluted, deconvolutedamu, amucleavage, cleavagetriphosphate, triphosphateprobes, probesphosphate, phosphatemass, massvaccinia, vacciniadiphosphate, diphosphateuncapped, uncappedthermostable
Rapid Analysis of Synthetic mRNA Cap Structure Using Ion-Pairing RPLC with the BioAccord LC-MS System
2021|Waters|Applications
Application NoteRapid Analysis of Synthetic mRNA CapStructure Using Ion-Pairing RPLC with theBioAccord LC-MS SystemJennifer M. Nguyen, Siu-Hong Chan, Bijoyita Roy, Martin Gilar, Brett Robb, Weibin Chen, Matthew A. LauberWaters Corporation, New England Biolabs Inc.Abstract Due to the crucial need to fight...
Key words
mrna, mrnabioaccord, bioaccordpremier, premiersurfaces, surfacesmaxpeak, maxpeakadsorb, adsorbdiisopropylethylamine, diisopropylethylaminedipea, dipeaacquity, acquityalkylamines, alkylamineshigh, highconfers, confersmodality, modalityvaccine, vaccinerecipient
ASMS 2021: Rapid Analysis of mRNA 5’-Capping with High-Resolution LC-MS
2021|Agilent Technologies|Posters
Poster ReprintASMS 2021Poster number FP568Rapid Analysis of mRNA 5’-Capping withHigh-Resolution LC-MSBrian Liau, PhD. 11AgilentTechnologies Inc., Santa Clara, CA IntroductionExperimentalIndustrial-scale production of mRNA has taken onrenewed importance due to the on-going pandemic.Efficient translation of mRNA to protein dependscritically on a 5’-terminal dinucleotide...
Key words
capping, cappingmrna, mrnaarca, arcauncapped, uncappedcapped, cappedvaccinia, vacciniatriphosphate, triphosphaternase, rnasethermostable, thermostablediphosphate, diphosphateenzyme, enzymechimeric, chimericcap, capidentities, identitiesintermediates