ASMS 2021: Rapid Analysis of Synthetic mRNA Cap Structure using Ion Pairing Reversed Phase Liquid Chromatography coupled to Mass Spectrometry | LabRulez LCMS
 

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Application NoteImproved Chromatographic Analysis ofOligonucleotides with ACQUITY PREMIEROligonucleotide BEH C18 ColumnsMartin Gilar, Zhimin Li, Jason MacLeanWaters Corporation AbstractThis application note illustrates sources of oligonucleotide loss due to adsorption on metalliccolumn hardware (frits). Solutions, columns, and best practices for improved oligonucleotideanalysis are...
Key words
oligonucleotide, oligonucleotideoligonucleotides, oligonucleotidesmiser, misersurfaces, surfacesmetallic, metallicfrit, fritadsorption, adsorptionuplc, uplcacquity, acquitypremier, premierstainless, stainlesssteel, steeloligodeoxythymidine, oligodeoxythymidinehps, hpsmaxpeak
[ APPLICATION NOTEBOOK ]APPLICATION SOLUTIONS FOROLIGONUCLEOTIDES Table of ContentsIntroduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4[ HPLC & UPLC Separations ]Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options...
Key words
oligonucleotides, oligonucleotidesuplc, uplcoligonucleotide, oligonucleotideacquity, acquityrnai, rnaiost, ostpromass, promassduplex, duplexduplexes, duplexeswaters, waterssirna, sirnaqda, qdarna, rnaanalysis, analysispairing
Application NoteBiopharmaRapid Analysis of mRNA 5' Cappingwith High Resolution LC/MSAuthorBrian LiauAgilent Technologies, Inc.IntroductionIndustrial-scale production of mRNA has received growing attention due to theongoing SARS-CoV-2 pandemic. mRNA vaccines have proven to be one of the mosteffective tools against the virus and...
Key words
mrna, mrnacapping, cappingchimeric, chimericamu, amudeconvoluted, deconvolutedrnase, rnasecleavage, cleavagetriphosphate, triphosphateprobes, probesphosphate, phosphatemass, massvaccinia, vacciniadiphosphate, diphosphateuncapped, uncappedthermostable
Application NoteRapid Analysis of Synthetic mRNA CapStructure Using Ion-Pairing RPLC with theBioAccord LC-MS SystemJennifer M. Nguyen, Siu-Hong Chan, Bijoyita Roy, Martin Gilar, Brett Robb, Weibin Chen, Matthew A. LauberWaters Corporation, New England Biolabs Inc.Abstract Due to the crucial need to fight...
Key words
mrna, mrnabioaccord, bioaccordpremier, premiersurfaces, surfacesadsorb, adsorbmaxpeak, maxpeakdiisopropylethylamine, diisopropylethylaminedipea, dipeaacquity, acquityalkylamines, alkylaminesconfers, confershigh, highmodality, modalityrecipient, recipientvaccine
 

Similar PDF

Toggle
Application NoteImproved Chromatographic Analysis ofOligonucleotides with ACQUITY PREMIEROligonucleotide BEH C18 ColumnsMartin Gilar, Zhimin Li, Jason MacLeanWaters Corporation AbstractThis application note illustrates sources of oligonucleotide loss due to adsorption on metalliccolumn hardware (frits). Solutions, columns, and best practices for improved oligonucleotideanalysis are...
Key words
oligonucleotide, oligonucleotideoligonucleotides, oligonucleotidesmiser, misersurfaces, surfacesmetallic, metallicfrit, fritadsorption, adsorptionuplc, uplcacquity, acquitypremier, premierstainless, stainlesssteel, steeloligodeoxythymidine, oligodeoxythymidinehps, hpsmaxpeak
[ APPLICATION NOTEBOOK ]APPLICATION SOLUTIONS FOROLIGONUCLEOTIDES Table of ContentsIntroduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4[ HPLC & UPLC Separations ]Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options...
Key words
oligonucleotides, oligonucleotidesuplc, uplcoligonucleotide, oligonucleotideacquity, acquityrnai, rnaiost, ostpromass, promassduplex, duplexduplexes, duplexeswaters, waterssirna, sirnaqda, qdarna, rnaanalysis, analysispairing
Application NoteBiopharmaRapid Analysis of mRNA 5' Cappingwith High Resolution LC/MSAuthorBrian LiauAgilent Technologies, Inc.IntroductionIndustrial-scale production of mRNA has received growing attention due to theongoing SARS-CoV-2 pandemic. mRNA vaccines have proven to be one of the mosteffective tools against the virus and...
Key words
mrna, mrnacapping, cappingchimeric, chimericamu, amudeconvoluted, deconvolutedrnase, rnasecleavage, cleavagetriphosphate, triphosphateprobes, probesphosphate, phosphatemass, massvaccinia, vacciniadiphosphate, diphosphateuncapped, uncappedthermostable
Application NoteRapid Analysis of Synthetic mRNA CapStructure Using Ion-Pairing RPLC with theBioAccord LC-MS SystemJennifer M. Nguyen, Siu-Hong Chan, Bijoyita Roy, Martin Gilar, Brett Robb, Weibin Chen, Matthew A. LauberWaters Corporation, New England Biolabs Inc.Abstract Due to the crucial need to fight...
Key words
mrna, mrnabioaccord, bioaccordpremier, premiersurfaces, surfacesadsorb, adsorbmaxpeak, maxpeakdiisopropylethylamine, diisopropylethylaminedipea, dipeaacquity, acquityalkylamines, alkylaminesconfers, confershigh, highmodality, modalityrecipient, recipientvaccine
 

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