Methods for the Anion Exchange Chromatographic Analysis of mRNAs
Applications | 2021 | WatersInstrumentation
Pharma & BiopharmaManufacturer
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Size and Purity Assessment of Single-Guide RNAs by Anion-Exchange Chromatography (AEX)
Application NoteSize and Purity Assessment of Single-GuideRNAs by Anion-Exchange Chromatography(AEX)Hua Yang, Stephan M. Koza, Ying Qing YuWaters CorporationAbstractSingle-guide RNA (sgRNA) is a critical element in the CRISPR/Cas9 Technology for gene editing, the size ofwhich usually ranges from 100 to 150...
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ASMS: Characterization of Intact mRNA Using Ion Pair-Reversed Phase-Time of Flight-MS, Size Exclusion Chromatography-Multi Angle Light Scattering, and Charge Detection Mass Spectrometry
Characterization of Intact mRNA Using Ion Pair-Reversed Phase-Time of Flight-MS, Size ExclusionChromatography-Multi Angle Light Scattering, and Charge Detection Mass SpectrometryPatrick Brophy1; Henry Shion1; Catalin E Doneanu1; Hua Yang1; Benjamin Draper2; Daniel Botamanenko2; Susan Abbatiello1; Andy Jarrell1; Stephan Koza1; Ying-Qing Yu1;...
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Salt Plug Injection Methods for Improved Anion Exchange Analyses of Large Nucleic Acids
Application NoteSalt Plug Injection Methods forImproved Anion ExchangeAnalyses of Large Nucleic AcidsSzabolcs Fekete, Matthew A. LauberWaters CorporationAbstractWith a burgeoning pipeline of nucleic acid-based therapies, there is a need forimproved analytical methods that can quickly confirm the concentration, integrity,and relative abundance...
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HPLC: FAST AND HIGH RECOVERY ANALYTICAL CHARACTERIZATION OF mRNAs USING WEAK ANION EXCHANGE AND ION PAIRING REVERSED PHASE CHROMATOGRAPHY
FAST AND HIGH RECOVERY ANALYTICALCHARACTERIZATION OF mRNAs USING WEAK ANIONEXCHANGE AND ION PAIRING REVERSED PHASE CHROMATOGRAPHYMateusz IMIOLEK1, Szabolcs FEKETE1, Matthew A. LAUBER21Waters Corporation, Geneva, Switzerland, 2Waters Corporation, Milford, MA, USAINTRODUCTIONANION EXCHANGE CHROMATOGRAPHY (AEX)AEX analyses of biomolecules often suffers from poor...
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