Methods for the Anion Exchange Chromatographic Analysis of mRNAs

Applications | 2021 | WatersInstrumentation
HPLC
Industries
Pharma & Biopharma
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Waters
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Application NoteSize and Purity Assessment of Single-GuideRNAs by Anion-Exchange Chromatography(AEX)Hua Yang, Stephan M. Koza, Ying Qing YuWaters CorporationAbstractSingle-guide RNA (sgRNA) is a critical element in the CRISPR/Cas9 Technology for gene editing, the size ofwhich usually ranges from 100 to 150...
Key words
aex, aexrnas, rnasassessment, assessmentanion, anionsize, sizepurity, purityexchange, exchangessrna, ssrnaguide, guidesingle, singlechromatography, chromatographyres, resssrnas, ssrnaspak, pakladder
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cdms, cdmsmrna, mrnamda, mdamals, malsmass, massintact, intactfluc, fluctechniques, techniquessec, sectof, tofvaccines, vaccinesdimer, dimercharge, chargeintensity, intensityminutes
Application NoteSalt Plug Injection Methods forImproved Anion ExchangeAnalyses of Large Nucleic AcidsSzabolcs Fekete, Matthew A. LauberWaters CorporationAbstractWith a burgeoning pipeline of nucleic acid-based therapies, there is a need forimproved analytical methods that can quickly confirm the concentration, integrity,and relative abundance...
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nucleic, nucleicplug, pluganion, anionexchange, exchangesalt, saltimproved, improvedacids, acidsanalyses, analyseslarge, largeinjection, injectioncarryover, carryovermrna, mrnamethods, methodsaex, aexmrnas
FAST AND HIGH RECOVERY ANALYTICALCHARACTERIZATION OF mRNAs USING WEAK ANIONEXCHANGE AND ION PAIRING REVERSED PHASE CHROMATOGRAPHYMateusz IMIOLEK1, Szabolcs FEKETE1, Matthew A. LAUBER21Waters Corporation, Geneva, Switzerland, 2Waters Corporation, Milford, MA, USAINTRODUCTIONANION EXCHANGE CHROMATOGRAPHY (AEX)AEX analyses of biomolecules often suffers from poor...
Key words
mrnas, mrnasaex, aexresidence, residencepairing, pairingmrna, mrnaweak, weakinteractions, interactionsgradient, gradientinitial, initialtime, timesolute, soluterna, rnaphase, phaseproportional, proportionalion