Methods for the Anion Exchange Chromatographic Analysis of mRNAs | LabRulez LCMS
 

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Anion-Exchange Chromatography for Determining Emptyand Full Capsid Contents in Adeno-Associated VirusHua Yang, Stephan Koza, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■Empty and full AAV capsid separationRecombinant adeno-associated virus (AAV) vector has been increasinglyand quantification by Protein-Pakused as a vehicle...
Key words
empty, emptycapsid, capsidcapsids, capsidsaav, aavaex, aexfull, fullvirus, virusadeno, adenoserotypes, serotypesanion, anionassociated, associateddetermining, determiningminutes, minutesexchange, exchangeres
Application NoteSize and Purity Assessment of Single-GuideRNAs by Anion-Exchange Chromatography(AEX)Hua Yang, Stephan M. Koza, Ying Qing YuWaters CorporationAbstractSingle-guide RNA (sgRNA) is a critical element in the CRISPR/Cas9 Technology for gene editing, the size ofwhich usually ranges from 100 to 150...
Key words
aex, aexrnas, rnasassessment, assessmentanion, anionsize, sizepurity, purityexchange, exchangessrna, ssrnaguide, guidesingle, singleres, reschromatography, chromatographypak, pakssrnas, ssrnasladder
Characterization of Intact mRNA Using Ion Pair-Reversed Phase-Time of Flight-MS, Size ExclusionChromatography-Multi Angle Light Scattering, and Charge Detection Mass SpectrometryPatrick Brophy1; Henry Shion1; Catalin E Doneanu1; Hua Yang1; Benjamin Draper2; Daniel Botamanenko2; Susan Abbatiello1; Andy Jarrell1; Stephan Koza1; Ying-Qing Yu1;...
Key words
cdms, cdmsmrna, mrnamda, mdamals, malsmass, massintact, intactfluc, fluctechniques, techniquessec, sectof, tofvaccines, vaccinesdimer, dimercharge, chargeintensity, intensityminutes
Application NoteBiopharmaceuticalsAnalysis of Oligonucleotides withIon Exchange Chromatography andAgilent Infinity II UHPLCAuthorsAbstractChae-Young Ryu andBrian LiauAgilent Technologies, Inc.Although oligonucleotide analysis is frequently conducted using ion-pairreversed‑phase chromatography, ion exchange chromatography can be better suitedfor certain analyses. This application note demonstrates the utility of...
Key words
mau, mausax, saxoligonucleotide, oligonucleotidemin, mintime, timeoligonucleotides, oligonucleotidesssdna, ssdnaladder, ladderbio, bioexchange, exchangecolumns, columnsagilent, agilentresolution, resolutionseparations, separationsnacl
 

Similar PDF

Toggle
Anion-Exchange Chromatography for Determining Emptyand Full Capsid Contents in Adeno-Associated VirusHua Yang, Stephan Koza, and Weibin ChenWaters Corporation, Milford, MA, USAAPPLICATION BENEFITS■■Empty and full AAV capsid separationRecombinant adeno-associated virus (AAV) vector has been increasinglyand quantification by Protein-Pakused as a vehicle...
Key words
empty, emptycapsid, capsidcapsids, capsidsaav, aavaex, aexfull, fullvirus, virusadeno, adenoserotypes, serotypesanion, anionassociated, associateddetermining, determiningminutes, minutesexchange, exchangeres
Application NoteSize and Purity Assessment of Single-GuideRNAs by Anion-Exchange Chromatography(AEX)Hua Yang, Stephan M. Koza, Ying Qing YuWaters CorporationAbstractSingle-guide RNA (sgRNA) is a critical element in the CRISPR/Cas9 Technology for gene editing, the size ofwhich usually ranges from 100 to 150...
Key words
aex, aexrnas, rnasassessment, assessmentanion, anionsize, sizepurity, purityexchange, exchangessrna, ssrnaguide, guidesingle, singleres, reschromatography, chromatographypak, pakssrnas, ssrnasladder
Characterization of Intact mRNA Using Ion Pair-Reversed Phase-Time of Flight-MS, Size ExclusionChromatography-Multi Angle Light Scattering, and Charge Detection Mass SpectrometryPatrick Brophy1; Henry Shion1; Catalin E Doneanu1; Hua Yang1; Benjamin Draper2; Daniel Botamanenko2; Susan Abbatiello1; Andy Jarrell1; Stephan Koza1; Ying-Qing Yu1;...
Key words
cdms, cdmsmrna, mrnamda, mdamals, malsmass, massintact, intactfluc, fluctechniques, techniquessec, sectof, tofvaccines, vaccinesdimer, dimercharge, chargeintensity, intensityminutes
Application NoteBiopharmaceuticalsAnalysis of Oligonucleotides withIon Exchange Chromatography andAgilent Infinity II UHPLCAuthorsAbstractChae-Young Ryu andBrian LiauAgilent Technologies, Inc.Although oligonucleotide analysis is frequently conducted using ion-pairreversed‑phase chromatography, ion exchange chromatography can be better suitedfor certain analyses. This application note demonstrates the utility of...
Key words
mau, mausax, saxoligonucleotide, oligonucleotidemin, mintime, timeoligonucleotides, oligonucleotidesssdna, ssdnaladder, ladderbio, bioexchange, exchangecolumns, columnsagilent, agilentresolution, resolutionseparations, separationsnacl
 

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