Back to Basics - Dispersion - Protocols for LC InstrumentsTechnické články | 2020 | ShimadzuInstrumentationHPLCKey wordsdispersion, featherstone, court, mill, road, 𝑡𝑅, zdv, measured, 𝑆𝑦𝑠𝑡𝑒𝑚, 𝑉𝑜𝑙𝑢𝑚𝑒, yield, volume, zero, union, dwell, length, effect, what, basics, switches, efficiency, excessive, protocols, injecting, valves, why, installed, tubing, essential, acetone, back, conditions, design, onto, cell, kit, example, operating, can, should, how, reduce, instruments, improved, chromatograms, column, available, two, low, flow
Back to Basics - Gradient Retention Factor, K*
Back to BasicsGradient Retention Factor, K*2020What is Gradient Retention Factor, k*?In isocratic separations, analyte retention time can be normalised to retention factor, k, whichallows a direct comparison between columns with different dimensions. This is possible due to theconstant mobile phase...
featherstone, featherstonecourt, courtmill, millroad, roadɸ𝑉, ɸ𝑉𝑉𝑚, 𝑉𝑚𝜀𝑡, 𝜀𝑡𝑡𝐺, 𝑡𝐺affect, affecttransferrable, transferrableimpractical, impracticalretentivity, retentivityseparate, separatebasics, basicsremember
Back to Basics - Explaining Resolution
Back to BasicsExplaining Resolution2020What is Resolution?Chromatography is the act of separating components in a mixture. There are differentmeasurements to determine the success of a separation, one of which is resolution (Rs). Resolutionis determined between two peaks of interest, which are...
featherstone, featherstonecourt, courtmill, millroad, roadionisable, ionisable𝑅𝑠, 𝑅𝑠alter, altermodifier, modifierorganic, organicresolution, resolutioncan, canequation, equationdifferent, different𝑑𝑝, 𝑑𝑝resolved
Back to Basics - Gradient Anatomy - Protocols for LC Instruments
Back to BasicsGradient AnatomyProtocols for LC Instruments2020Why is Gradient Chromatography Needed?Analytical samples are quite frequently made of complex analytes with varying physico-chemical properties,such as polarity. The analysis of these compounds using isocratic conditions could be impractical for a fewreasons:• There...
featherstone, featherstonecourt, courtmill, millroad, roadgradient, gradient𝑡𝐺, 𝑡𝐺ɸ𝑉𝑚𝑆, ɸ𝑉𝑚𝑆𝑃𝐶, 𝑃𝐶anatomy, anatomyalters, altersretentivity, retentivitypitfalls, pitfallsbasics, basicschromatography, chromatographyexperimentally
Back to Basics - Pump Linearity and Dwell Volume Measurements - Protocols for LC Instruments
Back to BasicsPump Linearity and DwellVolume MeasurementsProtocols for LC Instruments2020How is the Linearity of the Pump Measured?The linearity of the pump should be assessed between 0-100% using the conditions described in Figure 1. Thisis in order to ascertain the ability...
featherstone, featherstonecourt, courtdwell, dwellmill, millroad, roadmau, maucorr, corrwater, waterpump, pumpzdv, zdvvolume, volumeabs, absmeasured, measuredisocratic, isocraticgradient