Back to Basics - Pump Linearity and Dwell Volume Measurements - Protocols for LC InstrumentsTechnické články | 2020 | ShimadzuInstrumentationHPLCKey wordsfeatherstone, court, dwell, mill, road, mau, corr, water, pump, zdv, volume, abs, measured, isocratic, gradient, linearity, amax, proportionate, zero, mobile, union, amid, changing, hold, min, composition, ascertain, phase, basics, how, initial, processing, differs, linear, uracil, time, chromatogram, corresponds, schematic, response, conditions, plays, referred, method, protocols, sometimes, dissolved, reach, two, understand
Back to Basics - Gradient Retention Factor, K*
Back to BasicsGradient Retention Factor, K*2020What is Gradient Retention Factor, k*?In isocratic separations, analyte retention time can be normalised to retention factor, k, whichallows a direct comparison between columns with different dimensions. This is possible due to theconstant mobile phase...
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Back to Basics - Dispersion - Protocols for LC Instruments
Back to BasicsDispersionProtocols for LC Instruments2020What is Dispersion?Dispersion is a measure of analyte band broadening between the injector and detector, excludingthe contribution from the column. As a sample is injected into the flow path, the analyte bandbegins to spread by...
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Back to Basics - Explaining Resolution
Back to BasicsExplaining Resolution2020What is Resolution?Chromatography is the act of separating components in a mixture. There are differentmeasurements to determine the success of a separation, one of which is resolution (Rs). Resolutionis determined between two peaks of interest, which are...
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Back to Basics - Gradient Anatomy - Protocols for LC Instruments
Back to BasicsGradient AnatomyProtocols for LC Instruments2020Why is Gradient Chromatography Needed?Analytical samples are quite frequently made of complex analytes with varying physico-chemical properties,such as polarity. The analysis of these compounds using isocratic conditions could be impractical for a fewreasons:• There...
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