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Back to BasicsGradient Retention Factor, K*2020What is Gradient Retention Factor, k*?In isocratic separations, analyte retention time can be normalised to retention factor, k, whichallows a direct comparison between columns with different dimensions. This is possible due to theconstant mobile phase...
Key words
featherstone, featherstonecourt, courtmill, millroad, roadɸ𝑉, ɸ𝑉𝑉𝑚, 𝑉𝑚𝜀𝑡, 𝜀𝑡𝑡𝐺, 𝑡𝐺affect, affecttransferrable, transferrableimpractical, impracticalretentivity, retentivityseparate, separatebasics, basicsremember
Back to BasicsDispersionProtocols for LC Instruments2020What is Dispersion?Dispersion is a measure of analyte band broadening between the injector and detector, excludingthe contribution from the column. As a sample is injected into the flow path, the analyte bandbegins to spread by...
Key words
dispersion, dispersionfeatherstone, featherstonecourt, courtmill, millroad, road𝑡𝑅, 𝑡𝑅zdv, zdvmeasured, measured𝑆𝑦𝑠𝑡𝑒𝑚, 𝑆𝑦𝑠𝑡𝑒𝑚𝑉𝑜𝑙𝑢𝑚𝑒, 𝑉𝑜𝑙𝑢𝑚𝑒yield, yieldvolume, volumezero, zerounion, uniondwell
Back to BasicsExplaining Resolution2020What is Resolution?Chromatography is the act of separating components in a mixture. There are differentmeasurements to determine the success of a separation, one of which is resolution (Rs). Resolutionis determined between two peaks of interest, which are...
Key words
featherstone, featherstonecourt, courtmill, millroad, roadionisable, ionisable𝑅𝑠, 𝑅𝑠alter, altermodifier, modifierorganic, organicresolution, resolutioncan, canequation, equationdifferent, different𝑑𝑝, 𝑑𝑝resolved
Back to BasicsGradient AnatomyProtocols for LC Instruments2020Why is Gradient Chromatography Needed?Analytical samples are quite frequently made of complex analytes with varying physico-chemical properties,such as polarity. The analysis of these compounds using isocratic conditions could be impractical for a fewreasons:• There...
Key words
featherstone, featherstonecourt, courtmill, millroad, roadgradient, gradient𝑡𝐺, 𝑡𝐺ɸ𝑉𝑚𝑆, ɸ𝑉𝑚𝑆𝑃𝐶, 𝑃𝐶anatomy, anatomyalters, altersretentivity, retentivitypitfalls, pitfallsbasics, basicschromatography, chromatographyexperimentally
 

Similar PDF

Back to BasicsGradient Retention Factor, K*2020What is Gradient Retention Factor, k*?In isocratic separations, analyte retention time can be normalised to retention factor, k, whichallows a direct comparison between columns with different dimensions. This is possible due to theconstant mobile phase...
Key words
featherstone, featherstonecourt, courtmill, millroad, roadɸ𝑉, ɸ𝑉𝑉𝑚, 𝑉𝑚𝜀𝑡, 𝜀𝑡𝑡𝐺, 𝑡𝐺affect, affecttransferrable, transferrableimpractical, impracticalretentivity, retentivityseparate, separatebasics, basicsremember
Back to BasicsDispersionProtocols for LC Instruments2020What is Dispersion?Dispersion is a measure of analyte band broadening between the injector and detector, excludingthe contribution from the column. As a sample is injected into the flow path, the analyte bandbegins to spread by...
Key words
dispersion, dispersionfeatherstone, featherstonecourt, courtmill, millroad, road𝑡𝑅, 𝑡𝑅zdv, zdvmeasured, measured𝑆𝑦𝑠𝑡𝑒𝑚, 𝑆𝑦𝑠𝑡𝑒𝑚𝑉𝑜𝑙𝑢𝑚𝑒, 𝑉𝑜𝑙𝑢𝑚𝑒yield, yieldvolume, volumezero, zerounion, uniondwell
Back to BasicsExplaining Resolution2020What is Resolution?Chromatography is the act of separating components in a mixture. There are differentmeasurements to determine the success of a separation, one of which is resolution (Rs). Resolutionis determined between two peaks of interest, which are...
Key words
featherstone, featherstonecourt, courtmill, millroad, roadionisable, ionisable𝑅𝑠, 𝑅𝑠alter, altermodifier, modifierorganic, organicresolution, resolutioncan, canequation, equationdifferent, different𝑑𝑝, 𝑑𝑝resolved
Back to BasicsGradient AnatomyProtocols for LC Instruments2020Why is Gradient Chromatography Needed?Analytical samples are quite frequently made of complex analytes with varying physico-chemical properties,such as polarity. The analysis of these compounds using isocratic conditions could be impractical for a fewreasons:• There...
Key words
featherstone, featherstonecourt, courtmill, millroad, roadgradient, gradient𝑡𝐺, 𝑡𝐺ɸ𝑉𝑚𝑆, ɸ𝑉𝑚𝑆𝑃𝐶, 𝑃𝐶anatomy, anatomyalters, altersretentivity, retentivitypitfalls, pitfallsbasics, basicschromatography, chromatographyexperimentally
 

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