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Back to Basics - Pump Linearity and Dwell Volume Measurements - Protocols for LC Instruments

 

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Back to Basics Gradient Anatomy Protocols for LC Instruments 2020 Why is Gradient Chromatography Needed? Analytical samples are quite frequently made of complex analytes with varying physico-chemical properties, such as polarity. The analysis of these compounds using isocratic conditions could…
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Back to Basics Dispersion Protocols for LC Instruments 2020 What is Dispersion? Dispersion is a measure of analyte band broadening between the injector and detector, excluding the contribution from the column. As a sample is injected into the flow path,…
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Back to Basics Gradient Retention Factor, K* 2020 What is Gradient Retention Factor, k*? In isocratic separations, analyte retention time can be normalised to retention factor, k, which allows a direct comparison between columns with different dimensions. This is possible…
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Back to Basics Explaining Resolution 2020 What is Resolution? Chromatography is the act of separating components in a mixture. There are different measurements to determine the success of a separation, one of which is resolution (Rs). Resolution is determined between…
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