<p><strong>This guide explains how to select an appropriate size exclusion chromatography (SEC) column for the analysis of biologics. SEC separates molecules based on their hydrodynamic radius, reflecting their size and shape rather than molecular weight.</strong></p><ul><li>Molecules penetrate the pores of the stationary phase</li><li>There are no chemical interactions with the stationary phase</li><li>Larger molecules elute faster</li><li>Smaller molecules elute later</li></ul><h3><span><strong>Principle of SEC Separation</strong></span></h3><p>The separation mechanism is based on molecular size:</p><p><strong>Large molecules</strong><br>→ do not enter the pores<br>→ travel through the interstitial spaces between particles<br>→ elute earlier</p><p><strong>Small molecules</strong><br>→ enter the pores<br>→ follow a longer path<br>→ elute later</p><h3><span><strong>Key Parameters for Column Selection</strong></span></h3><h4><span><strong>1. Molecule Nature</strong></span></h4><p><strong>Start by evaluating:</strong></p><ul><li>hydrodynamic radius</li><li>molecular shape</li></ul><p><strong>Examples:</strong></p><ul><li>Peptides: ~0.7 nm</li><li>Large biomolecules (e.g., AAVs): ~25 nm</li><li>mRNA: elongated, non-globular structure</li></ul><h4><span><strong>2. Pore Size Selection</strong></span></h4><p>The pore size of the stationary phase should be:</p><ul><li><strong>3–4 times larger than the hydrodynamic radius of the target molecule</strong></li></ul><h4><span><strong>3. Particle Size</strong></span></h4><ul><li>≤ 3 µm</li><li>ensures high separation efficiency</li></ul><h4><span><strong>4. Stationary Phase Material</strong></span></h4><ul><li>Prefer <strong>mechanically robust media</strong>, such as silica</li><li>Capable of withstanding <strong>high back pressure</strong></li></ul><h4><span><strong>5. Surface Chemistry</strong></span></h4><ul><li><strong>Chemical surface inertness is critical</strong></li><li><strong>Diol chemistry is preferred</strong>, because:<ul><li>it minimizes interactions</li><li>it provides a <strong>highly hydrophilic surface</strong></li></ul></li></ul><h4><span><strong>6. Column Hardware</strong></span></h4><ul><li>Use <strong>bio-inert hardware</strong></li><li>ensures:<ul><li>minimal analyte loss</li><li>high reproducibility</li></ul></li></ul><h3><span><strong>Available SEC Column Options (</strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products?category=353&amp;manufacturerItems=50&amp;search=dsec"><span><strong>BioZen dSEC Family</strong></span></a><span><strong>)</strong></span></h3><h4><span><strong>1. </strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/2192"><span><strong>90 Å (9 nm)</strong></span></a></h4><ul><li>peptides</li><li>small proteins</li><li>aggregates up to ~50 kDa</li></ul><h4><span><strong>2. </strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1534"><span><strong>200 Å</strong></span></a></h4><ul><li>antibodies</li><li>antibody–drug conjugates</li><li>short oligonucleotides</li></ul><h4><span><strong>3. </strong></span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1535"><span><strong>700 Å (70 nm)</strong></span></a></h4><ul><li>AAVs</li><li>mRNA</li><li>large protein complexes (e.g., IgM)</li></ul><h3><span><strong>Column Characteristics</strong></span></h3><ul><li>silica-based particles</li><li>diol surface chemistry (hydrophilic, inert)</li><li>bio-inert hardware</li><li>designed for:<ul><li>high recovery</li><li>high reproducibility</li></ul></li></ul><h3><span><strong>Performance and Calibration</strong></span></h3><h4><span><strong>Proteins and AAVs</strong></span></h4><ul><li><strong>90 Å</strong> → low molecular weight (up to ~50 kDa)</li><li><strong>200 Å</strong> → medium range (up to ~450–500 kDa)</li><li><strong>700 Å</strong> → large molecules (including AAVs)</li></ul><h4><span><strong>RNA and DNA</strong></span></h4><ul><li>separation of molecules with non-globular shapes</li><li><strong>200 Å</strong> → smaller oligonucleotides</li><li><strong>700 Å</strong> → larger nucleic acid chains (up to ~3000–4000 nucleotides)</li></ul><h3><span><strong>Conclusion</strong></span></h3><p>Selecting the correct SEC column requires:</p><ul><li>understanding molecule size and shape</li><li>choosing an appropriate pore size</li><li>selecting suitable particle size</li><li>ensuring inert surface chemistry and bio-inert hardware</li></ul><p>This enables optimal separation of a wide range of biologics.</p>

How to Select the Right SEC Column for the Analysis of Biologics

<h4><span><strong>The science behind gummy candies</strong></span></h4><p><strong>Who can resist colourful, fruity gummy candies 🍬 with their signature chewy and elastic texture? While they may appear simple, their formulation is actually quite sophisticated. Each gummy is produced using a precisely balanced combination of gelling agents, sugars, acids, flavourings, and colourants, all working together to deliver the desired taste and texture.</strong></p><p>For instance, the characteristic chewiness of gummy sweets results from the interaction between gelatin and sugars. Even minor adjustments—such as changing the type or concentration of the gelling agent—can significantly alter the final product. In addition, manufacturers must meet regulatory and labeling requirements, including certifications like Halal compliance. This makes accurate and consistent monitoring of ingredients essential throughout production.</p><h4><span><strong>The importance of SEC fingerprinting</strong></span></h4><p>Size Exclusion Chromatography (SEC), also known as Gel Permeation Chromatography (GPC), offers a fast and reliable way to analyze gummy formulations. By separating molecules according to their hydrodynamic size, SEC generates a unique molecular “fingerprint” for each product.</p><p>This fingerprint enables manufacturers to:</p><ul><li><strong>confirm the type and origin</strong> of <strong>gelling agents</strong> (e.g., for Halal verification)</li><li>ensure <strong>batch-to-batch consistency</strong> and detect production variations</li><li><strong>monitor changes during storage</strong> to assess shelf life</li><li>evaluate <strong>low-molecular-weight compounds</strong> such as sugars in a semi-quantitative manner</li></ul><p>Overall, SEC fingerprinting supports consistent product quality and regulatory compliance, ensuring that every gummy maintains the same expected characteristics.</p><h4><span><strong>Influence of column selection on SEC performance</strong></span></h4><p>Column selection plays a crucial role in achieving high-quality separations and detailed molecular fingerprints. To illustrate this, porcine and bovine gelatin standards were analyzed using two different configurations:</p><ul><li>a single Multipore <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/products/1655"><strong>SuperOH-P column</strong></a></li><li>a coupled system of SuperOH-P 350 and SuperOH-P 150 columns</li></ul><p>Although both setups cover the same molecular <strong>weight range (100–1,000,000 Da)</strong>, the coupled configuration effectively doubles the separation length. This results in improved resolution, which was confirmed experimentally. The coupled columns provided clearer separation between porcine and bovine gelatin, producing more distinct and informative fingerprints.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_1_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_d81181cfb4.jpg" alt="KNAUER: Fig. 1 Overlay chromatograms measured with DAD at 230 nm, 20 µL. Dark blue: porcine reference standard; red: bovine reference standard; solid line: measured with column A; dotted line: measured with column set B. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_1_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_d81181cfb4.jpg 245w," sizes="100vw" width="1024" height="611"></p><h4><span><strong>Achieving reliable SEC fingerprints</strong></span></h4><p>Reproducibility is key when using SEC for quality control. The process begins with consistent sample preparation—maintaining identical dissolution procedures, concentrations, and filtration steps is essential, as even small variations can influence peak shape and overall results.</p><p>Beyond the column, the entire system setup is equally important. For example, <strong>combining</strong> a <strong>Refractive Index Detector</strong> (RID) with a <strong>Diode Array Detector (DAD)</strong> allows simultaneous detection of sugars, acids, and polymers within a single analysis.</p><p>Accurate peak identification further enhances reliability. By analyzing reference standards based on known ingredient compositions, chromatographic peaks can be correctly assigned, enabling detailed interpretation of both high- and low-molecular-weight components.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_2_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_37caf5ac59.jpg" alt="KNAUER: Fig. 2 Zoom overlay chromatograms measured with RID, 20 µL and column set B. Black: Katjes® Yoghurt Gums; turquoise: apple pectin diluted 1:10; dark green: preserving sugar 1:1 (sugar; citric acid; pectin; fully hydrogenated sunflower oil); dark blue: maltodextrin; pink: sodium potassium tartrate; yellow: potassium citrate; red: citric acid; purple: lactic acid; beige: lactose; orange: sucrose; green: glucose; grey: blank. Graphic by KNAUER. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_2_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_37caf5ac59.jpg 245w," sizes="100vw" width="1024" height="611"></p><p>Additionally, incorporating a flow marker helps correct for system-related variations, such as fluctuations in flow rate, thereby improving retention time stability and peak assignment accuracy. More details on the flow marker and its use in SEC are described in <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VTN_0043_knauer_gpc_sec_data_reproducibility_with_flow_marker_96ee6b3cd9.pdf"><strong>this technical note</strong></a>.</p><h4><span><strong>Verification of gelatin origin using SEC</strong></span></h4><p>Determining the source of gelatin is critical for proper labeling and compliance, particularly for Halal-certified products where porcine gelatin is not permitted.</p><p>To demonstrate SEC’s capability, two gummy samples with identical formulations but different gelatin sources were analyzed. One sample contained porcine gelatin, while the other used bovine gelatin. The resulting SEC fingerprints showed clear differences in the gelatin region, allowing unambiguous identification. The standard Haribo® Goldbears corresponded to the porcine reference, whereas the Halal version matched the bovine standard.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_3_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_and_column_set_B_12e99a43e7.jpg" alt="KNAUER: Fig. 3 Overlay chromatograms measured with DAD at 230 nm, 20 µL and column set B. Solid dark blue line: Haribo® Goldbears; solid red line: Halal Haribo® Goldbears; dotted dark blue line: porcine reference standard; dotted red line: bovine reference standard. Graphic by KNAUER. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_3_Overlay_chromatograms_measured_with_DAD_at_230_nm_20_L_and_column_set_B_12e99a43e7.jpg 245w," sizes="100vw" width="1024" height="611"></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/KNAUER_Fig_4_Overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_2_c29ea59bc4.jpg" alt="KNAUER: Fig. 4 Overlay chromatograms measured with RID, 20 µL and column set B. Solid dark blue line: Haribo® Goldbears; solid red line: Halal Haribo® Goldbears; dotted dark blue line: porcine reference standard; dotted red line: bovine reference standard. Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_KNAUER_Fig_4_Overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_2_c29ea59bc4.jpg 245w," sizes="100vw" width="1368" height="432"></p><p>This confirms that SEC is a reliable method for distinguishing gelatin origin.</p><h4><span><strong>Role of low-molecular-weight components</strong></span></h4><p>In addition to polymers such as gelatin, SEC can also reveal smaller molecules, including sugars and organic acids. While SEC is not a replacement for fully validated HPLC methods, it serves as a rapid screening tool for comparing samples and supporting quality control.</p><p>Using RID detection, differences in sugar composition become visible. For example, chromatograms showed a significantly higher sucrose signal in preserving sugar compared to gummy candies, reflecting formulation differences.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Knauer_Fig_5_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_06c072e069.jpg" alt="KNAUER: Fig. 5 Zoom overlay chromatograms measured with RID, 20 µL and column set B. Black: sucrose 1mg/ml; blue: Haribo® Goldbears; green: preserving sugar 1:1 (sugar; citric acid; pectin; fully hydrogenated sunflower oil). Graphic by KNAUER." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Knauer_Fig_5_Zoom_overlay_chromatograms_measured_with_RID_20_L_and_column_set_B_06c072e069.jpg 245w," sizes="100vw" width="1024" height="611"></p><h4><span><strong>Conclusion</strong></span></h4><p>SEC is a powerful analytical technique for monitoring product quality and ensuring process consistency in gummy candy production. By generating detailed molecular fingerprints, it enables manufacturers to verify ingredients, maintain consistency, and comply with regulatory requirements.</p><p>Importantly, this approach is not limited to gummy candies—it can be adapted to a wide range of products where molecular characterization is essential.</p><blockquote><ul><li>If you're planning to set up or optimize your GPC/SEC workflow, feel free to contact us at <strong>sales@knauer.net</strong>. Stay tuned for more exciting insights into the GPC/SEC world in our “GPC/SEC Made Easy” series.</li><li>For more information on the technical details, you can refer to <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/VFD_0195_sec_for_fingerprint_analysis_of_gummy_candies_ce5349cd95.pdf"><strong>this application note.</strong></a></li><li>For further information on this topic, please contact our author: <strong>wesolowski@knauer.net</strong></li></ul></blockquote>

GPC/SEC Made Easy – Fingerprint analysis of Gummy Candies

News from LabRulezLCMS Library - Week 17, 2026

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Based in Berlin, KNAUER is a medium-sized, owner-managed company that has been serving the sciences since 1962. We develop and manufacture scientific instruments of superior quality for liquid chromatography, including: Analytical HPLC/UHPLC, Preparative HPLC, Fast protein liquid chromatography (FPLC), Multi-column chromatography/Simulated moving bed (SMB) chromatography. High pressure dosing, in-line detection systems, and osmometry are additional business areas.

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Waters SunFire C8 HPLC Columns

Waters SunFire C8 columns are used for general purpose method development at low pH.

<p><strong>SunFire C8 columns are used for general purpose method development at low pH. The C8 ligand is preferred for some assays because it is less hydrophobic and therefore less retentive compared to SunFire C18.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: SunFire C8 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C8</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L7</li><li><strong>Carbon Load:</strong> 12 %</li></ul><h4><strong>The Waters SunFire C8 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters SunFire C8 HPLC Column&nbsp;with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: SunFire C8 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>SunFire Columns</strong></h3><h4><strong>Superior mass loading at low pH</strong></h4><p><strong>High efficiency at a low pH</strong> can be challenging. Waters SunFire HPLC Columns, available in <strong>analytical</strong> and <strong>preparative dimensions</strong>, help you do just that with state-of-the-art bonded silica designed for high mass loading, excellent low pH stability, superior peak shapes, and high efficiency.</p><p><strong>SunFire Analytical Columns</strong> are available in <strong>2.5 µm</strong> to <strong>10 µm </strong>particle sizes across two reversed-phase column chemistries, <strong>C18</strong> and <strong>C8</strong>, sure to meet your silica-based HPLC column requirements. For preparative applications, in addition to the C18 and C8 chemistries, SunFire silica is offered in <strong>5</strong> and <strong>10 µm particle sizes</strong> for <strong>preparative normal-phase purification</strong>.</p><h4><strong>Overview</strong></h4><ul><li><strong>Exceptional loading capacity </strong>for both analytical and preparative columns</li><li><strong>Excellent low pH stability </strong>for longer lifetimes</li><li><strong>Superior peak shapes </strong>for faster and easier integration</li><li><strong>Low mass spectral (MS) bleed</strong>, for enhanced analyte MS signal</li><li><strong>Seamless scalability from analytical</strong> to optimum bed density (OBD) <strong>preparative columns</strong></li><li><strong>Recommended Use:</strong> For increased mass loading with excellent column stability.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Sun_Fire_Columns_3f48bd770c/Waters-SunFire-Columns.jpg" alt="Waters: SunFire Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Sun_Fire_Columns_3f48bd770c/thumbnail_Waters-SunFire-Columns.jpg 242w," sizes="100vw" width="900" height="580"></p>

Waters SunFire Silica HPLC Columns

Waters SunFire Prep Silica columns are general purpose columns used under normal phase conditions.

<p><strong>SunFire Prep Silica columns are general purpose columns used under normal phase conditions.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: SunFire Silica HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> Silica</li><li><strong>Separation Mode: </strong>Normal Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> No</li><li><strong>Silanol Activity:</strong> High</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L3</li></ul><h3><strong>SunFire Columns</strong></h3><h4><strong>Superior mass loading at low pH</strong></h4><p><strong>High efficiency at a low pH</strong> can be challenging. Waters SunFire HPLC Columns, available in <strong>analytical</strong> and <strong>preparative dimensions</strong>, help you do just that with state-of-the-art bonded silica designed for high mass loading, excellent low pH stability, superior peak shapes, and high efficiency.</p><p><strong>SunFire Analytical Columns</strong> are available in <strong>2.5 µm</strong> to <strong>10 µm </strong>particle sizes across two reversed-phase column chemistries, <strong>C18</strong> and <strong>C8</strong>, sure to meet your silica-based HPLC column requirements. For preparative applications, in addition to the C18 and C8 chemistries, SunFire silica is offered in <strong>5</strong> and <strong>10 µm particle sizes</strong> for <strong>preparative normal-phase purification</strong>.</p><h4><strong>Overview</strong></h4><ul><li><strong>Exceptional loading capacity </strong>for both analytical and preparative columns</li><li><strong>Excellent low pH stability </strong>for longer lifetimes</li><li><strong>Superior peak shapes </strong>for faster and easier integration</li><li><strong>Low mass spectral (MS) bleed</strong>, for enhanced analyte MS signal</li><li><strong>Seamless scalability from analytical</strong> to optimum bed density (OBD) <strong>preparative columns</strong></li><li><strong>Recommended Use:</strong> For increased mass loading with excellent column stability.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Sun_Fire_Columns_3f48bd770c/Waters-SunFire-Columns.jpg" alt="Waters: SunFire Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Sun_Fire_Columns_3f48bd770c/thumbnail_Waters-SunFire-Columns.jpg 242w," sizes="100vw" width="900" height="580"></p>

Waters Symmetry C18 HPLC Columns

With Waters Symmetry C18 the monofunctionally bound C18 ligand included in these columns, you can increase efficiency and precision in your lab.

<p><strong>Primarily supports legacy methods. Symmetry columns give you confidence in the long-term compliance of your HPLC methods. No need to compromise on peak shape, selectivity, column-to-column and batch-to-batch reproducibility, or other critical performance characteristics. Available in a variety of particle sizes, chemistries and dimensions.</strong></p><p>With the <strong>Symmetry C18 Column</strong>, you will gain excellent <strong>reproducibility</strong> year after year. With each batch of new columns, there is no need to revalidate and redevelop techniques. The lab equipment enables the labor costs associated with standard analytical columns that require QC column testing to be reduced.</p><p>The <strong>Symmetry C18 Columns</strong> are manufactured in <strong>cGMP</strong>, <strong>ISO9001</strong>, and <strong>ISO13485</strong> certified facilities, ensuring that each step is monitored and the toughest criteria are maintained. They offer the tightest specifications in the market, ensuring consistent results every time. This is accomplished by designing Symmetry C18 Columns with high purity raw materials and column packaging techniques that provide the most repeatable HPLC column.</p><p>With the <strong>monofunctionally bound C18 ligand</strong> included in these columns, you can increase efficiency and precision in your lab. For optimum reproducibility, they additionally comprise a high purity base-deactivated silica. Symmetry C18 Columns also offer the industry's tightest ligand surface coverage (ligand density) standards, resulting in minimum resolution changes and more consistent batch-to-batch results.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Symmetry C18 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>300 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon Load:</strong> 8.5 %</li></ul><h4><strong>The Waters Symmetry C18 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters Symmetry C18 HPLC Column&nbsp;with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: Symmetry C18 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h5><strong>Waters Symmetry Shield RP18 HPLC Column</strong></h5><ul><li>These reversed-phase columns contain Waters patented embedded polar group technology that “shields” the silica’ residual silanol surface from highly basic analytes. The reduced silanol activity for the SymmetryShield RP18 column significantly improves peak shape and resolution.</li><li><strong>Bonding Technology: </strong>Shield RP18</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>Carbon Load:</strong> 17 %</li></ul><h5><strong>Waters Symmetry Shield RP18 HPLC Column with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><h3><strong>Symmetry HPLC Columns</strong></h3><h4><strong>Silica-based columns that exceed HPLC column performance expectations</strong></h4><p><strong>Reproducible HPLC columns</strong> are essential when <strong>developing analytical methods</strong> for <strong>pharmaceutical </strong>and <strong>biopharmaceutical products</strong>. Achieving the same chromatographic results over the lifetime of a new drug product is critical, and no other silica-based LC column brand can match the column-to-column and batch-to-batch reproducibility of the Symmetry family.</p><p>Available in column and guard formats, <strong>Symmetry Columns</strong> are manufactured using <strong>high purity silica</strong> and tightly controlled manufacturing processes to ensure that you receive a column that exceeds the standards for HPLC column performance.</p><h4><strong>Overview</strong></h4><ul><li>Deliver maximum reproducibility batch-to-batch and column-to-column</li><li>Provide superior peak shape</li><li>Achieve high recoveries of peptides and proteins</li><li>Manufactured using high purity silica and tightly controlled manufacturing processes</li><li><strong>Recommended Use:</strong> For rugged and reproducible HPLC column performance.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Symmetry_Columns_a2367d8095/Waters-Symmetry-Columns.jpg" alt="Waters: Symmetry Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Symmetry_Columns_a2367d8095/thumbnail_Waters-Symmetry-Columns.jpg 223w," sizes="100vw" width="700" height="490"></p><h5><strong>Rugged and reproducible HPLC column performance</strong></h5><p>With Symmetry HPLC Columns, you’ll achieve reliable column-to-column and batch-to-batch reproducibility. Symmetry’s narrow column specification ranges minimize variation, giving you the confidence that the methods you use today will continue to be used in the future.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Symmetry_HPLC_Colum_Unmatched_Year_to_year_Reproducibility_40b6d2de53.jpg" alt="Waters: Symmetry HPLC Columm - Unmatched Year to year Reproducibility" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Symmetry_HPLC_Colum_Unmatched_Year_to_year_Reproducibility_40b6d2de53.jpg 146w," sizes="100vw" width="620" height="664"></p><h5><strong>Superior peak shape</strong></h5><p>Symmetry Shield Columns feature the Waters patented embedded polar group technology that shields the silica's residual silanols from highly basic analytes and improves overall peak shape. Placing the embedded polar group close to the silica surface further reduces surface silanol activity, and the embedded polar group technology allows you to improve chromatographic peak shape and selectivity in the most challenging of method conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Symmetry_HPLC_Colum_Embedded_Polar_Group_Technology_improves_chromatographic_peak_shape_and_selectivity_519288463f.jpg" alt="Waters: Symmetry HPLC Column - Embedded Polar Group Technology improves chromatographic peak shape and selectivity" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Symmetry_HPLC_Colum_Embedded_Polar_Group_Technology_improves_chromatographic_peak_shape_and_selectivity_519288463f.jpg 161w," sizes="100vw" width="620" height="600"></p><h5><strong>Deliver high peptide recovery</strong></h5><p>All Symmetry300 columns are QC tested with peptide samples to ensure exceptional batch-to-batch reproducibility. These wide-pore (300 Å), reversed-phase chromatography columns deliver superior performance when simultaneously separating hydrophilic and hydrophobic peptides, neutral, and highly basic peptides. With a Symmetry300 column, you can expect better performance with either high or low mass loads.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Symmetry_HPLC_Colum_Compared_to_many_competitive_100_silica_based_C18_columns_Waters_proprietary_bonding_and_end_capping_technologies_61cde5dc5b.jpg" alt="Waters: Symmetry HPLC Column - Compared to many competitive 100% silica-based C18 columns, Waters proprietary bonding and end-capping technologies" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Symmetry_HPLC_Colum_Compared_to_many_competitive_100_silica_based_C18_columns_Waters_proprietary_bonding_and_end_capping_technologies_61cde5dc5b.jpg 245w," sizes="100vw" width="620" height="364"></p>

Waters Symmetry C8 HPLC Columns

Waters Symmetry C8 columns feature monofunctionally bonded C8 ligands on a high purity base-deactived silica. 

<p><strong>Symmetry C8 columns feature monofunctionally bonded C8 ligands on a high purity base-deactived silica. Symmetry columns have the tightest column specifications in the industry giving resulting highly reproducible chromatographic results.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Symmetry C8 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C8</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>100 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L7</li><li><strong>Carbon Load:</strong> 12 %</li></ul><h4><strong>The Waters Symmetry C8 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters Symmetry C8 HPLC Column&nbsp;with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: Symmetry C8 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h5><strong>Waters Symmetry Shield RP8 HPLC Column</strong></h5><ul><li>These reversed-phase columns contain Waters patented embedded polar group technology that “shields” the silica’ residual silanol surface from highly basic analytes. The reduced silanol activity for the SymmetryShield RP8 column significantly improves peak shape and resolution.</li><li><strong>Bonding Technology: </strong>Shield RP8</li><li><strong>Carbon Load:</strong> 15 %</li></ul><h5><strong>Waters Symmetry Shield RP8 HPLC Column with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><h3><strong>Symmetry HPLC Columns</strong></h3><h4><strong>Silica-based columns that exceed HPLC column performance expectations</strong></h4><p><strong>Reproducible HPLC columns</strong> are essential when <strong>developing analytical methods</strong> for <strong>pharmaceutical </strong>and <strong>biopharmaceutical products</strong>. Achieving the same chromatographic results over the lifetime of a new drug product is critical, and no other silica-based LC column brand can match the column-to-column and batch-to-batch reproducibility of the Symmetry family.</p><p>Available in column and guard formats, <strong>Symmetry Columns</strong> are manufactured using <strong>high purity silica</strong> and tightly controlled manufacturing processes to ensure that you receive a column that exceeds the standards for HPLC column performance.</p><h4><strong>Overview</strong></h4><ul><li>Deliver maximum reproducibility batch-to-batch and column-to-column</li><li>Provide superior peak shape</li><li>Achieve high recoveries of peptides and proteins</li><li>Manufactured using high purity silica and tightly controlled manufacturing processes</li><li><strong>Recommended Use:</strong> For rugged and reproducible HPLC column performance.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Symmetry_Columns_a2367d8095/Waters-Symmetry-Columns.jpg" alt="Waters: Symmetry Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Symmetry_Columns_a2367d8095/thumbnail_Waters-Symmetry-Columns.jpg 223w," sizes="100vw" width="700" height="490"></p>

Waters Symmetry C4 HPLC Columns

With Waters Symmetry C4 Column, you get the sharpest peaks for biomolecules and more peak capacity for complex tryptic maps. 

<p><strong>Primarily supports legacy methods. Symmetry columns give you confidence in the long-term compliance of your HPLC methods. No need to compromise on peak shape, selectivity, column-to-column and batch-to-batch reproducibility, or other critical performance characteristics. Available in a variety of particle sizes, chemistries and dimensions.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Symmetry C4 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C4</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Silanol Activity:</strong> Low</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>300 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L26</li><li><strong>Carbon Load:</strong> 2.8 %</li><li><strong>Application:</strong> Protein</li></ul><h3><strong>Symmetry HPLC Columns</strong></h3><h4><strong>Silica-based columns that exceed HPLC column performance expectations</strong></h4><p><strong>Reproducible HPLC columns</strong> are essential when <strong>developing analytical methods</strong> for <strong>pharmaceutical </strong>and <strong>biopharmaceutical products</strong>. Achieving the same chromatographic results over the lifetime of a new drug product is critical, and no other silica-based LC column brand can match the column-to-column and batch-to-batch reproducibility of the Symmetry family.</p><p>Available in column and guard formats, <strong>Symmetry Columns</strong> are manufactured using <strong>high purity silica</strong> and tightly controlled manufacturing processes to ensure that you receive a column that exceeds the standards for HPLC column performance.</p><h4><strong>Overview</strong></h4><ul><li>Deliver maximum reproducibility batch-to-batch and column-to-column</li><li>Provide superior peak shape</li><li>Achieve high recoveries of peptides and proteins</li><li>Manufactured using high purity silica and tightly controlled manufacturing processes</li><li><strong>Recommended Use:</strong> For rugged and reproducible HPLC column performance.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Symmetry_Columns_a2367d8095/Waters-Symmetry-Columns.jpg" alt="Waters: Symmetry Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Symmetry_Columns_a2367d8095/thumbnail_Waters-Symmetry-Columns.jpg 223w," sizes="100vw" width="700" height="490"></p>

Waters Nova-Pak C18 HPLC Columns

Waters Nova-Pak C18 columns are general purpose, silica-based, reversed-phase C18 columns that are based on 4 µm particle technology

<p><strong>Nova-Pak C18 columns are general purpose, silica-based, reversed-phase C18 columns that are based on 4 µm particle technology. Compared to a 5 µm column, Nova-Pak columns offer higher resolution, more efficient separations, and faster chromatography.</strong></p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg" alt="Waters: Nova-Pak C18 HPLC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Atlantis_d_C18_HPLC_Columns_d7a741df9f.jpg 234w," sizes="100vw" width="660" height="440"></p><h4><strong>Specifications</strong></h4><ul><li><strong>Chemistry:</strong> C18</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Silica</li><li><strong>pH Range Min: </strong>2 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Maximum Pressure: </strong>6000 psi (415 Bar)</li><li><strong>Endcapped:</strong> Yes</li><li><strong>Silanol Activity:</strong> Medium</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Pore Size: </strong>60 Å</li><li><strong>System: </strong>HPLC</li><li><strong>USP Classification:</strong> L1</li><li><strong>Carbon Load:</strong> 7 %</li></ul><h4><strong>The Waters Nova-Pak C18 HPLC Column is also available in these types:</strong></h4><h5><strong>Waters Nova-Pak C18 HPLC Column&nbsp;with eConnect Column Tag</strong></h5><ul><li>This column comes equipped with an electronic eConnect column tag containing unique column-specific information. These <strong>NFC-enabled device-tagged columns</strong> were developed to automatically identify, track and digitally trace column use history. eConnect columns can be used on <strong>any HPLC</strong>, but its <strong>advantages</strong> are unlocked when used with the <strong>Waters Alliance iS System</strong> running <strong>Empower software</strong>.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg" alt="Waters: Nova-Pak C18 HPLC Column with eConnect Column Tag" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_X_Bridge_BEH_C18_Column_with_e_Connect_Column_Tag_c056302ec7.jpeg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>Nova-Pak Columns</strong></h3><h4><strong>Scale-up from analytical to prep, simplified</strong></h4><p>Reducing solvent consumption while maintaining resolution for complex mixtures is important, but that’s a challenge with larger particle sizes. With the <strong>smaller particle sizes </strong>and <strong>shorter column lengths</strong> in Nova-Pak Columns, those problems are a thing of the past. The bonded phases of Nova-Pak Columns are available in <strong>4 µm</strong> and <strong>6 µm particle sizes</strong>, offering high resolution and faster, more efficient chromatography over columns packed with larger particle sizes.</p><p><strong>Nova-Pak Analytical Columns</strong> with <strong>4 µm particle size</strong> packing offer a range of <strong>length </strong>options with <strong>75, 100, 150, and 300 mm</strong> length steel columns. The <strong>semi-preparative</strong> <strong>Prep Nova-Pak HR Columns</strong> are packed with<strong> 6 µm particle size</strong> packings for unparalleled separation possibilities. The high-efficiency packing of Prep Nova-Pak HR Columns provides faster separations with less solvent for the added advantage of more concentrated fractions—reducing your preparative chromatography cost. To ensure batch-to-batch reproducibility, all <strong>Nova-Pak C18, C8, Cyano and Phenyl Columns</strong> are packed according to stringent quality control (QC) procedures in Waters <strong>cGMP</strong> manufacturing facility.</p><h4><strong>Overview</strong></h4><ul><li>Reduces solvent consumption while maintaining resolution for complex mixtures</li><li>Packed to stringent QC procedures to ensure batch-to-batch reproducibility</li><li>Chemistries include C18, C8, Cyano, and Phenyl stationary phases</li><li>Available in 4 µm analytical and 6 µm semi-preparative particle sizes</li><li><strong>Recommended Use:</strong> For higher resolution and faster chromatography.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Nova_Pak_Columns_fad066ad02/Waters-Nova-Pak-Columns.jpg" alt="Waters: Nova-Pak Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Nova_Pak_Columns_fad066ad02/thumbnail_Waters-Nova-Pak-Columns.jpg 245w," sizes="100vw" width="900" height="392"></p>

Waters ACQUITY APC Columns

Waters ACQUITY Advanced Polymer Chromatography (APC) Columns with novel capabilities for chromatographic efficiency, speed and resolution that cannot be matched by conventional GPC methods.

<h4><strong>ACQUITY APC AQ BEH Column</strong></h4><p>ACQUITY APC AQ columns are designed to provide highly efficient size-based separations for water soluble polymer characterization. The sorbent uses patented Ethylene Bridge Hybrid (BEH) technology to provide a mechanical strong packed bed that resists shinking and swelling for easy mobile phase solvent switching. The expected molecular weight range for this column is approximatley 1, 000 - 30, 000 g/mole.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Separation Mode:</strong> SEC/GPC</li><li><strong>Particle Substrate: </strong>Hybrid</li><li><strong>pH Range Min:</strong> 1 pH</li><li><strong>pH Range Max: </strong>8 pH</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>Medium</li><li><strong>Molecular Weight Range Min:</strong> 1000</li><li><strong>Molecular Weight Range Max:</strong> 30000</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Particle Size:</strong> 1,7 µm &nbsp;/ 2.5 µm&nbsp;</li><li><strong>Endfitting Type: </strong>Parker-style</li><li><strong>System:</strong> APC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>Technique: </strong>SEC</li></ul><h4><strong>ACQUITY APC XT BEH Column</strong></h4><p>ACQUITY APC XT columns are optimized to provide highly efficient size-based separations for organic solvent soluble polymer characterization. The sorbent uses patented Ethylene Bridge Hybrid (BEH) technology to provide a mechanical strong packed bed that resists shinking and swelling for easy mobile phase solvent switching.The expected molecular weight range for this column is approximatley 1,000 - 30,000 g/mole.</p><h4><strong>Specifications</strong></h4><ul><li><strong>Separation Mode:</strong> SEC/GPC</li><li><strong>Particle Substrate: </strong>Hybrid</li><li><strong>pH Range Min:</strong> 1 pH</li><li><strong>pH Range Max: </strong>12 pH</li><li><strong>Endcapped: </strong>No</li><li><strong>Silanol Activity: </strong>Low</li><li><strong>Molecular Weight Range Min:</strong> 1000</li><li><strong>Molecular Weight Range Max:</strong> 30000</li><li><strong>Particle Shape: </strong>Spherical</li><li><strong>Particle Size:</strong> 1,7 µm &nbsp;/ 2.5 µm&nbsp;</li><li><strong>Endfitting Type: </strong>Parker-style</li><li><strong>System:</strong> APC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>Technique: </strong>GPC</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_APC_AQ_BEH_Column_0c0d837b73.jpeg" alt="Waters: ACQUITY APC Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_APC_AQ_BEH_Column_0c0d837b73.jpeg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY APC Columns</strong></h3><h4><strong>Achieve superior speed and resolution for polymer characterization</strong></h4><p>Achieving superior performance and reliability with high resolution polymer characterization can be a challenge. <strong>ACQUITY Advanced Polymer Chromatography (APC) Columns</strong> are designed for polymer scientists like you, with novel capabilities for chromatographic efficiency, speed and resolution that cannot be matched by conventional GPC methods. With these columns, you can quantify and characterize polymer samples with confidence and accuracy while maximizing productivity.</p><p>The <strong>Ethylene Bridged Hybrid (BEH) particles</strong> used in ACQUITY APC Columns enable rapid solvent switching and the ability to use multiple conditions for the same bank of columns. Conventional polymeric stationary phases are dedicated to a single set of analytical conditions and solvent because the chromatographic bed is vulnerable to shrinking and swelling. By eliminating this undesirable behavior, ACQUITY APC Columns allow for maximum robustness and method development flexibility.</p><h4><strong>Overview</strong></h4><ul><li>Eliminate the shrinking and swelling that often occurs with polymeric stationary phases upon solvent switching with a stable chromatographic column bed&nbsp;</li><li>Achieve unmatched separation speed, resolution, and chromatographic efficiency from optimized pore volumes and particle size</li><li>Meet workflow requirements with 1.7 and 2.5 μm columns for organic and aqueous size-based separation</li><li>Achieve industry-leading column-to-column reproducibility with exceptionally reliable columns from a tightly controlled column manufacturing process</li><li><strong>Recommended Use: </strong>For rapid and accurate chromatographic characterization of synthetic polymer and macromolecular species.&nbsp;</li></ul><h4><strong>ACQUITY APC Column Featured Technologies</strong></h4><h5><strong>BEH Technology</strong></h5><ul><li>Rugged hybrid organic/inorganic base particle</li><li>Improved peak shape for bases- low silanol activity</li><li>Wider operating pH range for method development flexibility</li><li>Mechanical strength for maximum column efficiency at UHPLC operating pressures</li></ul><h4><strong>ACQUITY APC Column Characteristics</strong></h4><h5><strong>ACQUITY APC XT Columns</strong></h5><ul><li>Designed to provide high efficiency sized-based separations for organic soluble polymer characterization</li><li>Uses BEH particle technology</li><li>Stable column bed that resists shrinking and swelling for easy mobile-phase solvent switching</li><li>Features an extended temperature range up to 90 °C</li><li>Designed for organic solvent-based separations</li></ul><h5><strong>ACQUITY APC AQ Columns</strong></h5><ul><li>Designed to provide high efficiency sized-based separations for water soluble polymer characterization</li><li>Uses BEH particle technology</li><li>Robust separations up to 45 °C</li><li>Designed for aqueous-soluble polymer separations</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_APC_Columns_2268ceb5f5.jpeg" alt="Waters: ACQUITY APC Columns" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_APC_Columns_2268ceb5f5.jpeg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Unprecedented capacity for rapid solvent switching</strong></h4><p>Use multiple conditions for the same column bank with rapid solvent switching using the rigid hybrid particles in ACQUITY APC Chromatography Columns. Conventional polymeric phases are often dedicated to single analysis conditions because the chromatographic bed is vulnerable to shrinking and swelling that can be potentially harmful to the column. By eliminating this constraint, ACQUITY APC Chromatography Columns provide more freedom for method development and deliver advanced polymer characterization for a diverse range of samples.</p><h4><strong>Reduce calibration time by a factor of 3-5x and increase resolution</strong></h4><p>With long conventional Gel Permeation Chromatography (GPC) run times of up to one hour or more, analyses of standards and samples can be quite time-consuming. In addition to long GPC run times, the large extra-column volume of conventional GPC systems can result in peak bandspreading, reducing the resolution and thus accuracy of the calibration points. Combined with the lower dispersion ACQUITY APC System, small particle ACQUITY APC Columns deliver less bandspreading and narrower, sharper peaks compared to the conventional GPC system. Additionally, robust sub-3-µm APC Column Technology supports higher flow rate and backpressures, thereby improving resolution.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_A_comparison_of_run_times_and_resolution_for_polystyrene_standards_Mp_100_K_10_K_and_1_K_on_a_conventional_GPC_system_and_the_ACQUITY_APC_System_37cfb15ed5.jpeg" alt="Waters: A comparison of run times and resolution for polystyrene standards (Mp: 100K, 10K, and 1K) on a conventional GPC system and the ACQUITY APC System. " srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_A_comparison_of_run_times_and_resolution_for_polystyrene_standards_Mp_100_K_10_K_and_1_K_on_a_conventional_GPC_system_and_the_ACQUITY_APC_System_37cfb15ed5.jpeg 234w," sizes="100vw" width="1024" height="682"></p><h4><strong>Ensure reproducible column performance</strong></h4><p>Reproducible column performance is one of the most critical factors for achieving long-term method reliability during polymer characterization. Each step of our column manufacturing process is monitored and tightly controlled to maintain unmatched product reproducibility. Every test, from raw material characterization to final batch analysis, is designed to ensure that ACQUITY APC Columns perform the same from column-to-column, batch-to-batch, and year-to-year.</p>

Waters Sample & Collection Plate

Waters 96-well and 384-well plates are adherent to ANSI and SBS standards and are suitable for LC autosamplers and liquid handling robots.

<div><div><h4><strong>Broad selection of high-quality sample and collection plates</strong></h4></div></div><div><div><p><strong>Waters 96-well and 384-well plates are adherent to ANSI and SBS standards and are suitable for LC autosamplers and liquid handling robots. Waters plates are made from polypropylene (PP) for chemical resistance, and specialty 96-well plates with glass inserts are available for samples that are not suitable for polypropylene containers.</strong></p><h3><strong>Overview</strong></h3><div><div><div><div><div><div><ul><li>96- and 384-well collection plates and sample plates are SBS/ANSI compliant for robotic compatible systems&nbsp; &nbsp; &nbsp; &nbsp;</li><li>&nbsp;Constructed of polypropylene (PP) for chemical resistance</li><li>&nbsp;Glass inserts available for samples requiring glass surfaces</li><li>&nbsp;Heat seals and cap mats for plates and glass inserts</li></ul></div></div><div><div><h4><strong>Recommended Use</strong></h4><p>For general high-throughput LC and LC-MS work and for integration in (semi)-automated sample handling workflows.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/img_Waters_Sample_and_Collection_Plate_709693fdab.jpg" alt="imgWaters Sample &amp; Collection Plate." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_img_Waters_Sample_and_Collection_Plate_709693fdab.jpg 245w," sizes="100vw" width="1024" height="493"></p><h3><strong>Cap Mats</strong></h3><h4><strong>Silicone/PTFE &amp; Polypropylene Cap Mats</strong></h4><ul><li>Non-slit storage mats for long-term sample storage</li><li>Pre-slit silicone/PTFE and polypropylene cap mats for use in autosamplers for proper venting and sample draw accuracy with excellent resealing characteristics</li><li>Silicone/PTFE &amp; Polypropylene Cap Mats</li></ul><h3><strong>Heat Seals</strong></h3><h4><strong>Clear Polyester Heat Seals</strong></h4><ul><li>Can be used with most solvents &amp; buffers over a -80 °C to 80 °C temperature range</li><li>Requires a heat sealer to apply</li></ul><h4><strong>Aluminum Foil Heat Seals</strong></h4><ul><li>Made from a polyester/aluminum laminate</li><li>Ideal choice for long term storage to reduce evaporative loss</li><li>Aluminum layer reduces gas permeability of the seal</li><li>Can be used at temperatures from -200 °C to 90 °C</li><li>Requires a heat sealer to apply</li></ul><h4><strong>Adhesive Seals</strong></h4><ul><li>A polyolefin film with synthetic rubber adhesive</li><li>Ideal for use with protein and peptide samples and buffers</li><li>Resistant to small concentrations (0% to 30%) of polar organic solvents</li><li>Can be used at temperatures from -80 °C to 80 °C</li><li>Requires a heat sealer to apply</li></ul></div></div></div></div></div></div></div></div>

Waters ACQUITY UPLC C18 Columns

ACQUITY UPLC columns offer unmatched speed, resolution, and peak shape for a wide range of analytes. With advanced particle technologies and broad pH stability, they support efficient, reproducible separations in both routine and demanding applications.

<h5><strong>ACQUITY UPLC BEH C18 Columns</strong></h5><ul><li>Universal C18 column</li><li>Suitable for a diverse range of analytes</li><li>High efficiency</li><li>Industry-leading mobile phase pH 1-12 and temperature compatibility</li></ul><h5><strong>ACQUITY UPLC CSH C18 Columns</strong></h5><ul><li>Universal column choice for UV and MS applications</li><li>Suitable for a broad range of compounds</li><li>Alternate selectivity to BEH C18</li><li>Exceptional peak shape and increased load capacity</li><li>Suited for basic compounds at low pH</li></ul><h5><strong>ACQUITY UPLC HSS T3 Columns</strong></h5><ul><li>Ideal for the enhanced retention of polar and non-polar compounds</li><li>Compatible with 100% aqueous mobile phases</li><li>Mechanically stable at elevated pressures</li></ul><h5><strong>ACQUITY UPLC HSS C18 Columns</strong></h5><ul><li>General purpose silica-based C18 chemistry</li><li>Suitable for a broad range of compounds</li><li>Provides exceptional peak shapes and low pH stability</li><li>Delivers increased retention compared to hybrid organic/inorganic C18 columns</li></ul><h5><strong>ACQUITY UPLC HSS C18 SB Columns</strong></h5><ul><li>Low coverage silica-based C18 chemistry</li><li>Provides alternate selectivity for compounds impacted by silanophilic interactions</li><li>Increased silanol activity results in greater retention of basic compounds while reducing the retention of non-basic analytes</li><li>Provides exceptional peak shape and imparts Selectivity for Bases at low pH (SB)</li></ul><h4><strong>ACQUITY UPLC Application-Specific Column Characteristics</strong></h4><h5><strong>ACQUITY UPLC Peptide BEH C18 Columns</strong></h5><ul><li>Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH</li><li>Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns</li><li>Two pore sizes (130 Å and 300 Å) for a wide range of peptides</li></ul><h5><strong>ACQUITY UPLC Peptide CSH C18 Columns</strong></h5><ul><li>Outstanding peak capacities with formic acid for MS-based applications</li><li>Excellent performance with TFA for optical based applications</li><li>Less dependence on TFA or FA to achieve optimal peak capacity</li><li>Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities</li><li>At low pH with the positive charge on CSH, you get less secondary interactions with the particle</li></ul><h5><strong>ACQUITY UPLC Peptide HSS T3 Columns</strong></h5><ul><li>Separations that require silica-based selectivity</li><li>Separations that necessitate increased peptide retention</li><li>Ideally suited for small, hydrophilic peptides<br>&nbsp;</li></ul><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Waters ACQUITY UPLC BEH C8 Column

The Waters ACQUITY BEH C8 sorbent is less retentive compared to the ACQUITY BEH C18 sorbent. 

<p><strong>The ACQUITY BEH C8</strong> sorbent is <strong>less retentive compared to the ACQUITY BEH C18 </strong>sorbent. A method developer may prefer the speed offered by a less retentive column, while improving peak shape and chromatographic performance. The <strong>trifunctionally bonded BEH particles</strong> offer the widest usable <strong>pH range (1-12)</strong>, <strong>superior low pH stability</strong>, and <strong>ultra-low column bleed</strong>.</p><h5><strong>Specifications</strong></h5><ul><li><strong>Chemistry:</strong> C8</li><li><strong>Separation Mode: </strong>Reversed Phase</li><li><strong>Particle Substrate:</strong> Hybrid</li><li><strong>pH Range Min</strong>: 1 pH</li><li><strong>pH Range Max: </strong>12 pH</li><li><strong>Maximum Pressure: </strong>18000 psi (1240 Bar)</li><li><strong>Endcapped: </strong>Yes</li><li><strong>Silanol Activity: </strong>Low</li><li><strong>Particle Shape:</strong> Spherical</li><li><strong>Pore Size: </strong>130 Å</li><li><strong>System: </strong>UHPLC, UPLC</li><li><strong>Particle Technology:</strong> BEH</li><li><strong>USP Classification:</strong> L7</li><li><strong>Carbon Load: </strong>13 %</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg" alt="Waters: ACQUITY UPLC BEH C8 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_C8_Column_9c78ca994d.jpg 234w," sizes="100vw" width="660" height="440"></p><h3><strong>ACQUITY UPLC Columns</strong></h3><h5><strong>Increase speed, sensitivity, and resolution, without compromising data integrity</strong></h5><p>Increasing sample throughput, achieving unmatched resolution, and improving analyte sensitivity are priorities for your separation goals. With Waters ACQUITY UPLC Columns, you can meet all your method requirements and substantially reduce your analysis time and cost-per-sample.</p><p>Waters <strong>ACQUITY UPLC Columns’ sub-2-µm particle chemistries</strong> enable chromatographers to harness the separation efficiency improvements at increased flow rates of ultra-performance liquid chromatography (UPLC) technology, thereby improving analyte resolution and sensitivity as well as business productivity and profitability. &nbsp;</p><h4><strong>Overview</strong></h4><ul><li>Best-in-class column efficiency for superior separation speed, sensitivity, and resolution</li><li><strong>8 UPLC particles</strong> of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)</li><li><strong>22 stationary phases</strong>, including C18, Phenyl-Hexyl, C8, Embedded-Polar, C4, HILIC, Amide, Diol, Cyano, and PFP</li><li><strong>7 application-specific UPLC Column chemistries</strong> for SEC, proteins, peptides, oligonucleotides, glycan, and amino acid analysis</li><li><strong>More than 50 different column dimensions and configurations</strong>, with internal column diameters ranging from 1.0–4.6 mm and column lengths ranging from 30–300 mm</li><li><strong>Prolonged column lifetimes</strong> with optional <strong>VanGuard Cartridges</strong> specifically designed using ACQUITY stationary phases</li><li><strong>Recommended Use: </strong>For improved speed, sensitivity, and resolution in methods development.</li></ul><h4><strong>Particle technology</strong></h4><h5><strong>BEH - Ethylene Bridged Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC BEH </strong>1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable the separation scientist to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_BEH_technology_c07876b8a3.jpg" alt="Waters: BEH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_BEH_technology_c07876b8a3.jpg 170w," sizes="100vw" width="1050" height="964"></p><h5><strong>CSH - Charged Surface Hybrid Particle Technology</strong></h5><p><strong>ACQUITY UPLC CSH™</strong> 1.7 µm fully porous columns provide superior peak shape for basic analytes when using low ionic strength acidic mobile phases (e.g. 0.1% formic acid). With CSH Technology, the controlled low-level positive surface charge provides exceptional peak shape for basic analytes which can be achieved without the use of ion pairing reagents. Typically reversed-phase bonded phases have poor peak shape for basic compounds in formic acid even at analytical mass loads. Built on the rugged Ethylene Bridged Hybrid (BEH) particle, ACQUITY UPLC CSH phases can withstand the toughest operating conditions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_technology_ff4ddc1708.jpg" alt="Waters: CSH particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_technology_ff4ddc1708.jpg 169w," sizes="100vw" width="1049" height="968"></p><h5><strong>HSS - High Strength Silica (HSS) Particle Technology</strong></h5><p>ACQUITY UPLC HSS 1.8 µm fully porous columns provide expected silica particle-like performance to the UPLC family. The HSS particles’ high surface area, and lower pore volume provide increased retentivity over the hybrid particles. This robust particle technology is available in five uniquely different bonded phases, which truly maximize the selectivity space. The wide array of bonded phases associated with ACQUITY UPLC HSS Columns enable traditional hydrophobic reversed-phase interactions along with dipole-dipole interactions, aromatic interactions, and hydrogen bonding interactions.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_HSS_technology_b12d3ddfab.jpg" alt="Waters: HSS particle technology" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_HSS_technology_b12d3ddfab.jpg 162w," sizes="100vw" width="975" height="936"></p><h4><strong>Get superior peak shape for basic analytes</strong></h4><p>Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg" alt="Waters: Analysis of ziprasidone peroxide degradation on an ACQUITY UPLC CSH C18 Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Analysis_of_ziprasidone_peroxide_degradation_on_an_ACQUITY_UPLC_CSH_C18_Column_56909f1dc8.jpeg 245w," sizes="100vw" width="1024" height="485"></p><h4><strong>The ruggedness you want, the repeatability you need</strong></h4><p>ACQUITY UPLC BEH 1.7 µm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed-phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg" alt="Waters: ACQUITY UPLC BEH Columns can be used at the extremes of mobile phase pH (1-12) to enhance retention and specificity for complex samples that include mixtures of acidic, basic, and neutral analytes." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_ACQUITY_UPLC_BEH_Columns_can_be_used_at_the_extremes_of_mobile_phase_p_H_1_12_to_enhance_retention_and_specificity_for_complex_samples_that_include_mixtures_of_acidic_basic_and_neutral_analytes_5104d9a155.jpeg 195w," sizes="100vw" width="1024" height="823"></p><h4><strong>Achieve flexibility in method development</strong></h4><p>Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.</p><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg" alt="Waters: CSH Columns offer a wide range of selectivity differences (large S-values)." srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_CSH_Columns_offer_a_wide_range_of_selectivity_differences_large_S_values_733305abf8.jpeg 136w," sizes="100vw" width="1024" height="1173"></p>

Products focused on Consumables from Waters distributed by Waters Corporation (Europe) - page 2

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