Understanding the fundamentals of the on-off retention mechanism of oligonucleotides and their application to high throughput analysis

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 13th January 2025? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Thermo Fisher Scientific,</strong> <strong>Shimadzu</strong>,<strong> </strong>a presentation by <strong>Agilent Technologies</strong>, a manual by <strong>Metrohm,</strong> and a poster from <strong>ASMS </strong>by <strong>Waters Corporation</strong>!</p><h4><strong>1. Thermo Fisher Scientific: High-resolution DIA proteomics workflow for single-cell samples on the Orbitrap Astral mass spectrometer</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28638"><strong>Full PDF for download</strong></a></li></ul><p>Assess proteome coverage and sample throughput performance for single-cell samples using a library-free and library-based data-independent acquisition (DIA) method on the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer.</p><h5><strong>Introduction</strong></h5><p>In recent years, there has been a shift to analyzing smaller sample quantities, driven by the interest in profiling the proteome of individual cells. This increased focus on LC-MS analysis of limited sample amounts, e.g., single cells, requires the highest possible sensitivity. At the same time, throughput and chromatographic performance must be preserved to generate sufficiently high-quality data from large data sets to draw meaningful conclusions. This trend toward analyzing smaller sample quantities reflects the need to understand the heterogeneous nature of biology through the dissection of complex systems into their individual parts or, specifically, individual cells. Traditional proteomics approaches read out the average of a sample while single-cell proteomics provides insights into the exact nature of each of the components in such samples.</p><p>The Orbitrap Astral mass spectrometer delivers a new standard for single-cell samples with deeper proteome coverage and faster throughput. It enables these capabilities through the synchronized acquisition of high-resolution and high-dynamic range full scan spectra with the Thermo Scientific™ Orbitrap™ mass analyzer and high speed and sensitivity MS/MS spectra with the Thermo Scientific™ Astral™ mass analyzer. The Thermo Scientific™ Vanquish™ Neo UHPLC system delivers maximum performance in terms of precise, low flow rates and gradient formation, as well as low injection volumes—an important benefit for single-cell proteomics and limited sample amounts.</p><p>The Thermo Scientific™ μPAC™ Neo HPLC columns with novel micro-pillar array deliver highly reproducible chromatographic separation and increased sensitivity with maximum resolution.<br>The Thermo Scientific™ FAIMS Pro Duo interface enhances sensitivity by removing background signals.</p><p>In this technical note, we present a single-cell proteomics workflow (Figure 1). To first evaluate sensitivity and quantitative precision and accuracy and linearity of the workflow, a dilution series from 5 ng to 50 pg of the Thermo Scientific™ Pierce™ HeLa protein digest standard was analyzed.</p><h4><strong>2. Shimadzu: Automatic Optimization of Gradient Conditions by AI Algorithm - Consecutive Optimization at Different Column Oven Temperatures</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28642"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>The AI algorithm of LabSolutions MD can automatically optimize gradient conditions to greatly reduce labor of LC method development.</li><li>Gradient conditions are automatically optimized at different column oven temperatures consecutively.</li><li>Automatic optimization of gradient conditions can be applied not only to new method development, but also to existing method to efficiently improve resolution.</li></ul><h5><strong>Introduction</strong></h5><p>In the typical LC method development, the process begins with “preparation” which includes mobile phase preparation, column installation, and creation of analysis schedules, then the analysis is started. After that, the acquired data is analyzed and “preparation” for the subsequent analysis is carried out, followed by starting the next analysis again. The method development progresses by repeating these processes, but in addition to the significant time required to repeatedly create analysis schedules, expertise in chromatography is necessary to explore optimal conditions based on data analysis. In other words, typical method development requires “human intervention”. Therefore, eliminating human involvement and automating such method development processes would be desirable to improve labor efficiency. This article introduces an approach for automatically optimizing gradient conditions at different column oven temperatures consecutively, identifying the combination of temperature and gradient conditions that meet the resolution criteria.</p><h5><strong>Conclusion</strong></h5><p>Automatic optimization of gradient conditions using AI algorithm of LabSolutions MD was applied to a model sample (mixture of seven compounds of small molecule) at different column oven temperatures. As a result, the temperature and gradient conditions that met the resolution criteria were successfully explored. In method development, human intervention, such as analysis batch creation and data analysis, is required to optimize gradient conditions. LabSolutions MD can provide significant labor savings in this area. For more information on LabSolutions MD, please refer to the Technical Report <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/c190_e284_1ca86b48ea/c190-e284.pdf">“Efficient Method Development Based on Analytical Quality by Design with LabSolutions MD Software (C190-E284)”</a>.</p><h4><strong>3. Agilent Technologies: Optimize Your Polymer Analysis by Selecting the Ideal GPC/SEC Column</strong></h4><ul><li>Presentation</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28578"><strong>Full PDF for download</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4481"><strong>Recorded webinar</strong></a></li></ul><p>&nbsp;</p><ul><li><strong>GPC/SEC Separation Mechanism</strong></li><li><strong>Conventional GPC/SEC Workflow</strong></li><li><strong>Considerations for GPC/SEC Column Selection (Key questions to ask)</strong><ul><li>What polymer are you analysing?</li><li>Which solvent (or solvents) is your polymer soluble in?</li><li>What is the expected molecular weight range of your polymer?</li><li>What is the requirement for your analysis or what would you like to improve about your existing GPC/SEC separation?<ul><li>Resolution is important</li><li>Reproducibility of sample chromatography and results</li><li>Speed of analysis or sample throughput is something to improve on</li></ul></li></ul></li><li><strong>Further considerations</strong><ul><li>Know the properties of the sample</li><li>Be familiar with the properties of the columns being considered</li><li>It is important to balance polarities for the sample, solvent, and column packing</li></ul></li><li><strong>Common Column Chemistries for GPC/SEC</strong></li><li><strong>Agilent Magic Triangles</strong></li><li><strong>Column Selection: Porosity</strong></li><li><strong>Individual Pore Versus MultiPorous Particle</strong></li><li><strong>Column Selection: Particle size</strong></li><li><strong>Columns for Low Polarity Organic Eluents</strong></li><li><strong>Columns for Medium Polarity Organic Eluents</strong></li><li><strong>Columns for High Temperature GPC/SEC</strong></li><li><strong>Columns for Fluorinated Organic Eluents</strong></li><li><strong>Columns for Aqueous Eluents: Neutral or weak Anions</strong></li><li><strong>Columns for Aqueous Eluents: Polycations and Strong Polyanions</strong></li><li><strong>Column Selection: Additional Considerations</strong></li><li><strong>Columns in Series</strong></li><li><strong>Wrongly Coupled Columns</strong></li><li><strong>Combination of Individual Pore Size Columns</strong></li><li><strong>Column Selection and Importance of Solvent Choice</strong></li><li><strong>Solvent Selection</strong></li><li><strong>Successful Solvent Choice</strong></li><li><strong>Eluent Modification in Organic GPC</strong></li><li><strong>Common Questions Around GPC/SEC Standards</strong></li><li><strong>Agilent Polymer Calibration Standard Offerings</strong></li><li><strong>Well Characterized Polymer Standards</strong></li><li><strong>Easivial Certificate of Analysis</strong></li><li><strong>Common Questions Around SEC Standards</strong></li><li><strong>Installation and Care of Your Column</strong></li><li><strong>Before Your Analysis</strong></li><li><strong>Column Selection – Additional considerations</strong></li><li><strong>System Tubing</strong></li><li><strong>Your GPC/SEC Toolbox</strong></li></ul><h4><strong>4. Metrohm: Installation Instruction for ProfIC Vario 7 Cation</strong></h4><ul><li>Manual</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28584"><strong>Full PDF for download</strong></a></li></ul><p>The ProfIC Vario 7 Cation is a Professional IC Vario system with Inline Dilution and Inline Dialysis for the fully automatic determination of cations in high concentrations in matrices containing emulsions. This system enables users to dilute the sample and eliminate droplets of fat or organic solvents or particles prior to the analysis.</p><h4><strong>5. Waters Corporation: IMPROVED TISSUE HOMOGENIZATION AND SPE-BASED SAMPLE PREPARATIONS FOR THE QUANTITATIVE LC-MS ANALYSIS OF OLIGONUCLEOTIDE THERAPEUTICS</strong></h4><ul><li>Poster/ASMS</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/27580"><strong>Full PDF for download</strong></a></li></ul><p>Efficient extraction of drug analytes is a critical aspect of drug metabolism and pharmacokinetics (DMPK) studies. This has long applied to small molecules and still applies to the bioanalysis of oligonucleotides. Oligonucleotide drugs and their metabolites must be quantified within both biofluids and tissue samples. The newest of oligonucleotide drugs are both extensively modified and conjugated. These modified residues and conjugate moieties can complicate extraction recovery and reproducibility. In this work, we report several key insights on how to achieve improved extractions. Protocols for solvent-assisted proteinase K sample pre-treatments are investigated using a weak anion exchange (WAX) microplate-based solid phase extraction (SPE) device. Direct injection LC-MS quantitation is demonstrated for all three antisense oligonucleotides (ASOs).</p><h5><strong>CONCLUSIONS</strong></h5><ul><li>Selective extraction and elution of ASO oligonucleotides is feasible from multiple tissue types.</li><li>Extracted ASOs are amenable for either full scan mode analysis on benchtop small footprint TOF MS or MRM analysis by Triple Quad MS</li><li>Reproducible and repeatable data were collected even without the application of an internal standard</li><li>One novel aspect of the SPE eluate is that no further sample preparation is required for subsequent LC-MS analysis saving time and avoiding sample losses during drying and reconstitution</li><li>Eluted nucleic acid components can be readily analyzed by regular ion-pairing reversed phase chromatography and UV/MS-based detection and quantitation</li><li>This robust tissue extraction protocols for improved LC-MS based bioanalysis of ASO drugs was repeatable and highly reproducible across multiple batches of SPE sorbent, providing confidence in the suitability of the optimized protocols</li></ul>

News from LabRulezLCMS Library - Week 3, 2025

<p><strong>In the week of January 20, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over&nbsp;</p><p><strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Characterization and sequence mapping of large RNA and mRNA therapeutics using HRAM</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4580">Registration</a></div></li></ul></div><p>The presentation showcases the innovative partial T1 digest strategy developed at the University of Sheffield for sequencing mRNA therapeutics.</p></div><h5><strong>2.&nbsp;SelectScience/Agilent Technologies: From Start to Finish: How Agilent Can Support Better Decisions in IVT mRNA-Based Biopharmaceutical Analysis</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4674">Registration</a></div></li></ul></div><p>We will discuss how automated electrophoresis and LC/MS systems solutions from Agilent provide reliable insights for IVT mRNA workflows at many steps.</p></div><h5><strong>3.&nbsp;Phenomenex: LC Troubleshooting Quick Guide: Spot Problems Fast &amp; Fix Them Like a Pro!</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4676">Registration</a></div></li></ul></div><p>In this webinar - General troubleshooting approach, HPLC troubleshooting steps and diagnosing tips and preventive maintenance and column care.</p></div><h5><strong>4.&nbsp;Thermo Fisher Scientific: State-of-the-art E&amp;L is not rocket science</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4581">Registration</a></div></li></ul></div><p>Ulli Honester, a team leader from Solvias, presented on advancements in mass spectrometry for identifying extractables and leachables (E&amp;L).</p></div><h5><strong>5.&nbsp;Waters Corporation: Empower Tips: Ask Me Anything About Navigating Data Governance - Risk and Vigilant Monitoring</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4550">Registration</a></div></li></ul></div><p>We will explore critical aspects of data governance and critical thinking, insights and strategies to help navigate the current environment, evolving your approach and modernizing your way of working.</p></div><h5><strong>6.&nbsp;Evosep Biosystems: A beginner’s guide to Evosep 2025</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4548">Registration</a></div></li></ul></div><p>This webinar is your opportunity to gain a comprehensive understanding of the Evosep One’s key features and how it can seamlessly integrate into your current processes.</p></div><h5><strong>7.&nbsp;Shimadzu Corporation: ASTM D8421 and D8535: Cost Effective Methods for Rapid PFAS Testing in Wastewater and Soil</strong></h5><div><div><ul><li><div>Th, 23.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4465">Registration</a></div></li></ul></div><p>Discover simplified standardized methods for PFAS analysis based on co-solvation as sample preparation.</p></div>

Webinars LabRulezLCMS Week 4/2025

Article

Webinars

Application

Scientific article

Various

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Separation Science

Agilent Technologies

Technology Networks

Shimadzu Corporation

Mestrelab Research

Waters Corporation

<p><strong>We’ll explore how outlier setup can help you focus on samples and compounds that require your attention, ensuring accurate and reliable results.</strong></p><p>Additionally, you’ll learn how viewing compounds and calibration at a glance can expedite data review, making the process faster and more intuitive.</p><p>Finally, we’ll demonstrate how easily you can summarize your results in comprehensive reports or export them to Excel, streamlining your workflow and enhancing productivity.</p><p>Don’t miss this opportunity to enhance your data analysis skills with MassHunter Quant!</p><blockquote><p><strong>Presenter:</strong> Peter Kornas (LC/MS Applikationsspezialist, Agilent Technologies)</p><p>Peter Kornas has more than 10 years of experience in the development and validation of analytical methods for the determination of pesticides, mycotoxins and contaminants using LC/MS/MS. Before joining Agilent as an LC/MS application specialist in 2018, he worked as an analytical chemist in the food industry. During this time, he was responsible for the development and validation of analytical methods and worked in close coordination with Regulatory Affairs to ensure compliance with the legal requirements of the LC/MS method portfolio.</p></blockquote>

Agilent Technologies: MassHunter Masterclass Months

MassHunter Quant – A Fast and Easy Way from Data to Results

Join us and discover how quickly a quantitation method can be set up, enabling efficient data processing.

<p><strong>Join us for an insightful webinar where we will explore the critical aspects of differentiating good from bad data and how this distinction can significantly influence your results. From screening to quantification, discover the value of targeted data analysis workflows using non-targeted data with our innovative software and hardware.</strong></p><p>Find by Formula algorithm. Learn to identify the most suitable MS/MS approach for your experiments, initiate quantitation for your compounds of interest, and incorporate retention times (RTs) and MS/MS spectra into a custom database and library.</p><p>This session is designed to enhance your understanding and application of Q-TOF data analysis, ensuring robust and reliable outcomes.</p><blockquote><p><strong>Presenter:</strong> Hannah Florance (Agilent Technologies)</p><p>Hannah has had multiple roles in the life sciences, starting as a protein chemist, before moving into proteomics and then metabolomics. A major part of her time working in academia involved running LC and GCMS core facilities supporting projects ranging from synthetic biology to cancer research. She is currently an LCMS applications specialist for Agilent.<br><br><strong>Presenter:</strong> Stefan Schaebler (Application Specialist LC/MS, Agilent Technologies)</p><p>Stefan completed his PhD in Biology, specializing in LC-MS-based lipidomics and metabolomics. Following his doctoral studies, he joined Agilent Technologies as an LC/MS Application Specialist. In this role, Stefan focuses on small molecule applications using LC-QTOF, with a strong emphasis on software tools and data interpretation.</p></blockquote>

MassHunter Qual – The Solid Pillar in Q-TOF Data Analysis

We will explore the critical aspects of differentiating good from bad data and how this distinction can significantly influence your results.

<p><strong>Join our upcoming webinar on the MassHunter Quant Screener Tool to discover how to harness the power of quantitative analysis for effective screening and identification of potential suspect compounds.</strong></p><p>This session will provide in-depth guidance on creating methods from PCDL databases, evaluating result parameters, and generating straightforward summary reports. Additionally, learn how retention time (RT) prediction can enhance your screening process by significantly accelerating and simplifying large database searches.</p><p>Don’t miss this opportunity to elevate your analytical capabilities!</p><blockquote><p><strong>Presenter:</strong> &nbsp;Jonathan Spencer (Application Specialist, Agilent Technologies)</p><p>Jonathan studied BSc Natural Sciences at the University of Nottingham and later went on to Sheffield Hallam University to study a MSc in Forensic Science.</p><p>Following graduation, he spent 2 years as a Confirmatory Analyst in an anti-doping and forensic toxicology lab, before joining Agilent as an LC/MS Applications Scientist. At Agilent, he focuses on small molecule applications using LC/TQ and LC/Q-TOF in fields of like food, forensic and environmental analysis.</p></blockquote>

MassHunter Quant Screener Tool

Discover how to harness the power of quantitative analysis for effective screening and identification of potential suspect compounds. 

<p><strong>Join us on the hunt for the needle in the haystack!</strong></p><p>This session will delve into best practices for untargeted feature finding, focusing on achieving the optimal balance between sample coverage and processing speed using Profinder and the new MassHunter Explorer. We will explore techniques to differentiate between features and noise by applying the right filters for quality checking your data. Additionally, the webinar will cover essential aspects of compound identification, including database matching, formula generation, and structure elucidation.</p><p>This is a must-attend event for anyone looking to enhance their analytical capabilities and streamline their data processing workflows.</p><blockquote><p><strong>Presenter:</strong> &nbsp;Stefan Schaebler (Application Specialist LC/MS, Agilent Technologies)</p><p>Stefan completed his PhD in Biology, specializing in LC-MS-based lipidomics and metabolomics. Following his doctoral studies, he joined Agilent Technologies as an LC/MS Application Specialist. In this role, Stefan focuses on small molecule applications using LC-QTOF, with a strong emphasis on software tools and data interpretation.</p></blockquote>

Finding the Needle in the Haystack Part I: From Complex Samples to Compounds of Interest

The best practices for untargeted feature finding, focusing on achieving the optimal balance between sample coverage and processing speed.

From Complex Samples to Compounds of Interest

<p><span lang="EN-US" xml:lang="EN-US" data-scheme-color="@323130,," data-usefontface="false" data-contrast="none"><strong>Automated lab workflows often require many interactions with pre-existing in-house and commercial software systems. This webinar uses the real example of building a purification factory, to give some insights on how this can be done with Mestrelab tools in conjunction with in-house and commercial software.</strong></span></p><div data-aos-delay="0" data-aos-duration="1000"><div><p><span lang="EN-US" xml:lang="EN-US" data-usefontface="false" data-contrast="none">Join us for an insightful webinar where we’ll demonstrate how to integrate Mestrelab’s tools into a complex IT ecosystem to automate lab workflows. Using a real-world example, we'll show you how to build a purification factory that seamlessly connects in-house and commercial software systems.</span></p><p data-ccp-props="{&quot;335551550&quot;:1,&quot;335551620&quot;:1,&quot;335559683&quot;:0,&quot;335559685&quot;:0,&quot;335559731&quot;:0,&quot;335559737&quot;:0,&quot;335562764&quot;:2,&quot;335562765&quot;:1,&quot;335562766&quot;:4,&quot;335562767&quot;:10,&quot;335562768&quot;:4,&quot;335562769&quot;:0}"><span data-usefontface="false" data-contrast="none"><strong>Who Should Attend?</strong></span>​</p><ul style="font-weight:400;"><li data-charcodes="8226" data-font="Arial,Sans-Serif" data-buautonum="8" data-aria-posinset="1" data-aria-level="1"><span data-usefontface="false" data-contrast="none"><strong>Lab Managers and Scientists</strong> looking to automate and enhance lab workflows</span>​</li><li data-charcodes="8226" data-font="Arial,Sans-Serif" data-buautonum="8" data-aria-posinset="2" data-aria-level="1"><span data-usefontface="false" data-contrast="none"><strong>IT and Automation Specialists</strong> in life sciences or chemical industries seeking integration solutions</span>​</li><li data-charcodes="8226" data-font="Arial,Sans-Serif" data-buautonum="8" data-aria-posinset="3" data-aria-level="1"><span data-usefontface="false" data-contrast="none"><strong>Anyone interested</strong> in embedding Mestrelab tools into a powerful, scalable IT infrastructure</span></li></ul><blockquote><p><strong>Presenter:</strong> John Hollerton (Senior Scientific Director, Mestrelab Research)</p><p><span data-teams="true" dir="ltr">John has over 40 years of experience in the scientific and informatics fields at GSK and its predecessor companies. He has managed various analytical functions, including NMR, X-ray crystallography, mass spectrometry, physical chemistry, and chromatography, and has expertise in chemical structure representation and registration. In informatics, he led projects to create a global scientific data management system and wrote software for instrument control, data transformation, and analysis. His team developed software to automate LCMS purification factories in the US and UK.&nbsp;</span></p></blockquote></div></div>

Mestrelab: Building a Purification Factory: Embedding Mestrelab Tools into a Rich IT Infrastructure

Building a Purification Factory: Embedding Mestrelab Tools into a Rich IT Infrastructure

We’ll demonstrate how to integrate Mestrelab’s tools into a complex IT ecosystem to automate lab workflows.

Embedding Mestrelab Tools into a Rich IT Infrastructure

<div><div><p><strong>Therapeutic synthetic oligonucleotides represent unique analytical challenges in late-stage development. To meet the stringent regulatory requirements, highly specific and sensitive methods such as liquid chromatography-mass spectrometry (LC/MS) must be used. However, neither chromatography nor mass spectrometry data systems have the capabilities, both in function and in data integrity, for the typical QC analyst end user to run a highly complex method such as purity, assay, and impurity profiling for therapeutic oligos such as anti-sense oligonucleotides.</strong></p><p>In this presentation, we present a paradigm shift in software with Oligo Analysis Accelerator for OpenLab CDS. This application streamlines the analytical workflow by using a fit-for-purpose user interface while leveraging existing data integrity and compliance architecture within OpenLab CDS.</p><h4>By attending this webinar, you will:</h4><ul><li><strong>Understand the fundamentals of ion-pair reversed-phase LC/MS for oligonucleotides</strong></li><li><strong>Discover how a single quadrupole detector is used for impurity profiling of anti-sense oligonucleotides</strong></li><li><strong>Learn how fit-for-purpose software for LC/MS analysis in QC lot release can save time and enhance quality</strong></li></ul><h5>Who should attend</h5><ul><li>Analytical chemists interested in cutting-edge software for large molecule late-stage development</li><li>Analytical research and development labs interested in developing robust methods for single-stranded oligo therapeutics</li><li>Process scientists who rely on in-process control purity results to optimize synthesis and purification for oligos</li><li>Quality assurance and quality control personnel supporting oligo testing labs</li></ul><blockquote><p><strong>Presenter:</strong> Brian Rivera (Software Product Manager, Agilent Technologies)</p><p>Brian Rivera is a software product manager at Agilent Technologies with 20 years of experience in HPLC analysis of large molecules.</p></blockquote></div></div>

Separation Sciences: Boost Efficiency in Oligonucleotide QC with Software Designed for the Job

Boost Efficiency in Oligonucleotide QC with Software Designed for the Job

In this presentation, we present a paradigm shift in software with Oligo Analysis Accelerator for OpenLab CDS.

Oligonucleotide QC with Software Designed for the Job

<p><strong>Manual sample preparation has its limitations. As sample throughput increases, so does fatigue, leading to a decline in data quality. This issue is significant across various markets but is especially critical in drug development and the production of therapeutics in biotechnology.</strong></p><p>However, employing automation can enhance data consistency and improve reproducibility. Additionally, automation reduces the need for costly sample reruns and frees up users’ time for other tasks.</p><p><strong>Attend this webinar to:</strong></p><ul><li>Discover the benefits of automation and identify who should consider adopting it</li><li>Explore comprehensive workflow solutions that guide you from sample preparation to data-driven decisions, tailored to biopharma and proteomics applications</li><li>Learn how the updated AssayMAP Citation Index assists users in finding published methods that utilize the AssayMAP Bravo for biopharma applications</li><li>See how compliant software can facilitate the transition of methods from non-compliant to compliant labs, such as moving from early to late stages of drug discovery</li></ul><blockquote><p><strong>Presenter:</strong> Kenda LJ Evans, PhD (Automation Workflow Specialist, Agilent Technologies)</p><p>Kenda received her Ph.D. from The University of Texas Health Science Center in San Antonio in the field of Pharmacology with a focus on the signal transduction pathway for the Serotonin 1A (5-HT1A) receptor. Kenda’s post-doctoral work occurred at the University of Houston.</p></blockquote>

Technology Networks: Automation Answers to Your Liquid Handling Challenges

Automation Answers to Your Liquid Handling Challenges

Discover the benefits of automation and identify who should consider adopting it

<p><strong>This is the third in a three-part Ask Me Anything webinar series on processing data. There are numerous ways to process data in Empower Software. It depends on several things, such as the type of data (e.g., 2D versus 3D data) and the type of method (e.g., LC versus GPC/SEC).</strong></p><p>One of the big challenges is the task of reviewing all the results that are generated. It becomes particularly cumbersome when you want to review results from different versions of Empower Software. Empower Data Viewer (EDV) can simplify these tasks. You can overlay chromatograms from different Result Sets from different versions of Empower Software.</p><p><strong>In Part 3 of this series, we will discuss:</strong></p><ul><li>Processing sample sets, injections, and channels</li><li>Processing with the acquisition method set, a specified method set, or a processing method</li><li>Re-processing data</li><li>Empower Data Viewer – learn how to view and explore processed data in near real-time</li></ul><p>Please join us for a lively discussion as we explore this topic. As always, please bring your questions for this exclusive opportunity to ask our experts anything about Empower Software.</p><blockquote><p><strong>Presenter:</strong> Neil Lander (Principal Product Manager, Informatics, Waters Corporation)</p><p>Neil has worked for Waters Corporation since 1994 and has held a variety of positions including Technical Support Specialist, Technical Trainer, Manager Customer Education and currently, Principal Marketing Manager focusing on Informatics solutions. He provides internal support for Empower Chromatography Data System and is focused on developing laboratory software solutions that help organizations achieve their scientific and operational objectives. Prior to joining Waters, he was an analytical chemist where he gained experience in both the cosmetics and specialty chemical industries.</p><p><strong>Presenter:</strong> Zoe Chung (Principal Product Marketing Manager, Waters Corporation)</p></blockquote><div><blockquote><p>Zoe Chung has more than eight years of marketing experience, specializing in life sciences software marketing. As a Principal Product Marketing Manager at Waters, she focuses on the commercialization of informatics products as part of the Empower Solutions Portfolio.</p></blockquote></div>

Waters: Empower Tips: Ask Me Anything About Processing Data - Part 3

Empower Tips: Ask Me Anything About Processing Data - Part 3

There are numerous ways to process data in Empower Software

<p><strong>Are you ready to transform your pharmaceutical operations? Don’t miss our exclusive session on Shimadzu Digital Classrooms and begin your journey towards Pharma digital transformation (DX). Discover how Shimadzu’s pioneering AI technologies and digital solutions are ushering in a new era of pharma innovation.</strong></p><p>In this informative session, explore how Shimadzu is reshaping the drug development landscape. Learn how our innovative tools effectively streamline workflows, enhance lab efficiency, and maintain strict compliance with regulatory standards.</p><p>Whether you’re a forward-thinking researcher, a quality assurance professional, or a strategic decision-maker, this session is your gateway to mastering AI and digital transformation. Equip yourself with the expertise and resources to stay ahead in the industry.</p><p>This is an opportunity not to be missed – Register Now!</p><p><strong>Learning Objectives:&nbsp;</strong></p><ul><li>Understand how to stay conforming to the Pharma regulations and USP 621 changes with Shimadzu Living Laboratory</li><li>Explore Shimadzu AI and Automation to elevate your lab workflows effectively</li><li>Learn about the AI features of Shimadzu Ai-series and Nexera series</li><li>Gain insights into efficient quality control strategies with Shimadzu Data Management and Software</li></ul><blockquote><p><strong>Presenter: </strong>Liw Wan Tung (Assistant Market Manager (Pharma) Shimadzu (Asia Pacific) Pte Ltd)</p><p>Wan Tung is the LC Product Manager and Pharmaceutical market in-charge at Shimadzu Asia Pacific Pte Ltd. She is the LC expert with more than 15 years of working experience in analytical science, specializing in method development of various LC and LCMS applications. In 2012, she joined Shimadzu to develop end-to-end workflow solutions for the customers. She has supported a multitude of applications from impurity analysis to drug discovery and biopharmaceutical applications. Prior to joining Shimadzu, Wan Tung started her professional career as a QAQC chemist in Pharmaceutical multinational company. Wan Tung earned her bachelor’s honour's degree in chemistry back in 2009.</p><p><strong>Presenter:</strong> Kanimozhi Sivarajan (Informatics Manager Shimadzu (Asia Pacific) Pte Ltd)</p><p>Dr. Kani is having 10 years of experince in Laboratory Management and Informatics &nbsp;Systems. She is serving as Informatics Manager at Shimadzu Asia Pacific, Singapore, &nbsp;providing Lab Informatics Solutions such as LabSolutions CS, LIMS and other QMS applications. She is supporting customers from various segments on implementing laboratory automation and responsible for providing consultation on streamlining Lab Workflows, Regulatory Compliance requirements for Electronic Data Management and Lab Practices/Analysis.&nbsp;</p></blockquote>

Shimadzu:  Realizing Pharma DX: Discover Shimadzu’s AI-Driven Solutions And Automation

 Realizing Pharma DX: Discover Shimadzu’s AI-Driven Solutions And Automation

Discover how Shimadzu’s pioneering AI technologies and digital solutions are ushering in a new era of pharma innovation.

Pharma DX: Shimadzu’s AI-Driven Solutions And Automation

<p><strong>Data helps power the analytical laboratory. However, converting vast amounts of data into actionable decisions and confidence in business critical operations is extremely challenging.</strong></p><p>Existing solutions require custom system integrations that are expensive and difficult to maintain. Join us as we launch our newest cloud software solution, waters_connect Data Intelligence, specifically developed to help overcome these challenges by proving near-real time, in depth dashboards and powerful purpose-built analytics on your Empower<sup>&nbsp;</sup>Software data, helping you to:</p><ul><li>Improve productivity</li><li>Reduce risk</li><li>Save money</li><li>Accelerate time-to-market</li></ul><p><strong fr-original-style="">Be confidently audit ready, access deeper insights, and improve your laboratory operations.</strong></p><blockquote><p><strong fr-original-style="">Presenter: </strong>Jonathan Scott (Director of Product Management, Connected Science, Waters Corporation)</p><p>Prior to joining Waters in 2012, Jonathan worked in the regulated bioanalytical space focusing on developing and validating small and large molecule LC-MS methods. While at Waters, Jonathan has held multiple roles within product management including a focus on mass detection and biopharmaceutical software.</p><p><strong fr-original-style="">Presenter:&nbsp;</strong>Ben Dearnaley (Principal Product Owner, Connected Science, Waters Corporation)</p><p>Ben has worked at Waters for 17 years, and 14 years in Pharma prior to that. His area of interest as both a customer and at Waters has been on how to leverage Empower meta-data into analytics and business intelligence which helps Empower stakeholders understand better how their laboratories are working, such as understanding system and resource utilization, laboratory workflows and potential data integrity issues. Ben was a key individual involved with EDS365 and is now working as a Cloud Product Owner with a focus on bringing Empower analytics into the Cloud.</p></blockquote>

Waters: Level-up your Empower data with in-depth dashboards and powerful custom data analytics

LCMS webinars focusing on Software | LabRulez LCMS

Level-up your Empower data with in-depth dashboards and powerful custom data analytics