Understanding the fundamentals of the on-off retention mechanism of oligonucleotides and their application to high throughput analysis

<p><strong>Our Library never stops expanding. What are the most recent contributions to LabRulezLCMS Library in the week of 13th January 2025? Check out new documents from the field of liquid phase, especially HPLC and LC/MS techniques!</strong></p><blockquote><p><span style="font-size:20px;">👉 </span><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/library"><span style="font-size:20px;"><strong>SEARCH THE LARGEST REPOSITORY OF DOCUMENTS ABOUT LCMS AND RELATED TECHNIQUES</strong></span></a></p></blockquote><blockquote><p><strong>👉 Need info about different analytical techniques?</strong> Peek into <a target="_blank" rel="noopener noreferrer" href="https://gcms.labrulez.com/library"><strong>LabRulezGCMS</strong></a> or <a target="_blank" rel="noopener noreferrer" href="https://icpms.labrulez.com/library"><strong>LabRulezICPMS</strong></a> libraries.</p></blockquote><p>This week we bring you applications by <strong>Thermo Fisher Scientific,</strong> <strong>Shimadzu</strong>,<strong> </strong>a presentation by <strong>Agilent Technologies</strong>, a manual by <strong>Metrohm,</strong> and a poster from <strong>ASMS </strong>by <strong>Waters Corporation</strong>!</p><h4><strong>1. Thermo Fisher Scientific: High-resolution DIA proteomics workflow for single-cell samples on the Orbitrap Astral mass spectrometer</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28638"><strong>Full PDF for download</strong></a></li></ul><p>Assess proteome coverage and sample throughput performance for single-cell samples using a library-free and library-based data-independent acquisition (DIA) method on the Thermo Scientific™ Orbitrap™ Astral™ mass spectrometer.</p><h5><strong>Introduction</strong></h5><p>In recent years, there has been a shift to analyzing smaller sample quantities, driven by the interest in profiling the proteome of individual cells. This increased focus on LC-MS analysis of limited sample amounts, e.g., single cells, requires the highest possible sensitivity. At the same time, throughput and chromatographic performance must be preserved to generate sufficiently high-quality data from large data sets to draw meaningful conclusions. This trend toward analyzing smaller sample quantities reflects the need to understand the heterogeneous nature of biology through the dissection of complex systems into their individual parts or, specifically, individual cells. Traditional proteomics approaches read out the average of a sample while single-cell proteomics provides insights into the exact nature of each of the components in such samples.</p><p>The Orbitrap Astral mass spectrometer delivers a new standard for single-cell samples with deeper proteome coverage and faster throughput. It enables these capabilities through the synchronized acquisition of high-resolution and high-dynamic range full scan spectra with the Thermo Scientific™ Orbitrap™ mass analyzer and high speed and sensitivity MS/MS spectra with the Thermo Scientific™ Astral™ mass analyzer. The Thermo Scientific™ Vanquish™ Neo UHPLC system delivers maximum performance in terms of precise, low flow rates and gradient formation, as well as low injection volumes—an important benefit for single-cell proteomics and limited sample amounts.</p><p>The Thermo Scientific™ μPAC™ Neo HPLC columns with novel micro-pillar array deliver highly reproducible chromatographic separation and increased sensitivity with maximum resolution.<br>The Thermo Scientific™ FAIMS Pro Duo interface enhances sensitivity by removing background signals.</p><p>In this technical note, we present a single-cell proteomics workflow (Figure 1). To first evaluate sensitivity and quantitative precision and accuracy and linearity of the workflow, a dilution series from 5 ng to 50 pg of the Thermo Scientific™ Pierce™ HeLa protein digest standard was analyzed.</p><h4><strong>2. Shimadzu: Automatic Optimization of Gradient Conditions by AI Algorithm - Consecutive Optimization at Different Column Oven Temperatures</strong></h4><ul><li>Technical note</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28642"><strong>Full PDF for download</strong></a></li></ul><h5><strong>User Benefits</strong></h5><ul><li>The AI algorithm of LabSolutions MD can automatically optimize gradient conditions to greatly reduce labor of LC method development.</li><li>Gradient conditions are automatically optimized at different column oven temperatures consecutively.</li><li>Automatic optimization of gradient conditions can be applied not only to new method development, but also to existing method to efficiently improve resolution.</li></ul><h5><strong>Introduction</strong></h5><p>In the typical LC method development, the process begins with “preparation” which includes mobile phase preparation, column installation, and creation of analysis schedules, then the analysis is started. After that, the acquired data is analyzed and “preparation” for the subsequent analysis is carried out, followed by starting the next analysis again. The method development progresses by repeating these processes, but in addition to the significant time required to repeatedly create analysis schedules, expertise in chromatography is necessary to explore optimal conditions based on data analysis. In other words, typical method development requires “human intervention”. Therefore, eliminating human involvement and automating such method development processes would be desirable to improve labor efficiency. This article introduces an approach for automatically optimizing gradient conditions at different column oven temperatures consecutively, identifying the combination of temperature and gradient conditions that meet the resolution criteria.</p><h5><strong>Conclusion</strong></h5><p>Automatic optimization of gradient conditions using AI algorithm of LabSolutions MD was applied to a model sample (mixture of seven compounds of small molecule) at different column oven temperatures. As a result, the temperature and gradient conditions that met the resolution criteria were successfully explored. In method development, human intervention, such as analysis batch creation and data analysis, is required to optimize gradient conditions. LabSolutions MD can provide significant labor savings in this area. For more information on LabSolutions MD, please refer to the Technical Report <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/c190_e284_1ca86b48ea/c190-e284.pdf">“Efficient Method Development Based on Analytical Quality by Design with LabSolutions MD Software (C190-E284)”</a>.</p><h4><strong>3. Agilent Technologies: Optimize Your Polymer Analysis by Selecting the Ideal GPC/SEC Column</strong></h4><ul><li>Presentation</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/28578"><strong>Full PDF for download</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4481"><strong>Recorded webinar</strong></a></li></ul><p>&nbsp;</p><ul><li><strong>GPC/SEC Separation Mechanism</strong></li><li><strong>Conventional GPC/SEC Workflow</strong></li><li><strong>Considerations for GPC/SEC Column Selection (Key questions to ask)</strong><ul><li>What polymer are you analysing?</li><li>Which solvent (or solvents) is your polymer soluble in?</li><li>What is the expected molecular weight range of your polymer?</li><li>What is the requirement for your analysis or what would you like to improve about your existing GPC/SEC separation?<ul><li>Resolution is important</li><li>Reproducibility of sample chromatography and results</li><li>Speed of analysis or sample throughput is something to improve on</li></ul></li></ul></li><li><strong>Further considerations</strong><ul><li>Know the properties of the sample</li><li>Be familiar with the properties of the columns being considered</li><li>It is important to balance polarities for the sample, solvent, and column packing</li></ul></li><li><strong>Common Column Chemistries for GPC/SEC</strong></li><li><strong>Agilent Magic Triangles</strong></li><li><strong>Column Selection: Porosity</strong></li><li><strong>Individual Pore Versus MultiPorous Particle</strong></li><li><strong>Column Selection: Particle size</strong></li><li><strong>Columns for Low Polarity Organic Eluents</strong></li><li><strong>Columns for Medium Polarity Organic Eluents</strong></li><li><strong>Columns for High Temperature GPC/SEC</strong></li><li><strong>Columns for Fluorinated Organic Eluents</strong></li><li><strong>Columns for Aqueous Eluents: Neutral or weak Anions</strong></li><li><strong>Columns for Aqueous Eluents: Polycations and Strong Polyanions</strong></li><li><strong>Column Selection: Additional Considerations</strong></li><li><strong>Columns in Series</strong></li><li><strong>Wrongly Coupled Columns</strong></li><li><strong>Combination of Individual Pore Size Columns</strong></li><li><strong>Column Selection and Importance of Solvent Choice</strong></li><li><strong>Solvent Selection</strong></li><li><strong>Successful Solvent Choice</strong></li><li><strong>Eluent Modification in Organic GPC</strong></li><li><strong>Common Questions Around GPC/SEC Standards</strong></li><li><strong>Agilent Polymer Calibration Standard Offerings</strong></li><li><strong>Well Characterized Polymer Standards</strong></li><li><strong>Easivial Certificate of Analysis</strong></li><li><strong>Common Questions Around SEC Standards</strong></li><li><strong>Installation and Care of Your Column</strong></li><li><strong>Before Your Analysis</strong></li><li><strong>Column Selection – Additional considerations</strong></li><li><strong>System Tubing</strong></li><li><strong>Your GPC/SEC Toolbox</strong></li></ul><h4><strong>4. Metrohm: Installation Instruction for ProfIC Vario 7 Cation</strong></h4><ul><li>Manual</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/paper/28584"><strong>Full PDF for download</strong></a></li></ul><p>The ProfIC Vario 7 Cation is a Professional IC Vario system with Inline Dilution and Inline Dialysis for the fully automatic determination of cations in high concentrations in matrices containing emulsions. This system enables users to dilute the sample and eliminate droplets of fat or organic solvents or particles prior to the analysis.</p><h4><strong>5. Waters Corporation: IMPROVED TISSUE HOMOGENIZATION AND SPE-BASED SAMPLE PREPARATIONS FOR THE QUANTITATIVE LC-MS ANALYSIS OF OLIGONUCLEOTIDE THERAPEUTICS</strong></h4><ul><li>Poster/ASMS</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/paper/27580"><strong>Full PDF for download</strong></a></li></ul><p>Efficient extraction of drug analytes is a critical aspect of drug metabolism and pharmacokinetics (DMPK) studies. This has long applied to small molecules and still applies to the bioanalysis of oligonucleotides. Oligonucleotide drugs and their metabolites must be quantified within both biofluids and tissue samples. The newest of oligonucleotide drugs are both extensively modified and conjugated. These modified residues and conjugate moieties can complicate extraction recovery and reproducibility. In this work, we report several key insights on how to achieve improved extractions. Protocols for solvent-assisted proteinase K sample pre-treatments are investigated using a weak anion exchange (WAX) microplate-based solid phase extraction (SPE) device. Direct injection LC-MS quantitation is demonstrated for all three antisense oligonucleotides (ASOs).</p><h5><strong>CONCLUSIONS</strong></h5><ul><li>Selective extraction and elution of ASO oligonucleotides is feasible from multiple tissue types.</li><li>Extracted ASOs are amenable for either full scan mode analysis on benchtop small footprint TOF MS or MRM analysis by Triple Quad MS</li><li>Reproducible and repeatable data were collected even without the application of an internal standard</li><li>One novel aspect of the SPE eluate is that no further sample preparation is required for subsequent LC-MS analysis saving time and avoiding sample losses during drying and reconstitution</li><li>Eluted nucleic acid components can be readily analyzed by regular ion-pairing reversed phase chromatography and UV/MS-based detection and quantitation</li><li>This robust tissue extraction protocols for improved LC-MS based bioanalysis of ASO drugs was repeatable and highly reproducible across multiple batches of SPE sorbent, providing confidence in the suitability of the optimized protocols</li></ul>

News from LabRulezLCMS Library - Week 3, 2025

<p><strong>In the week of January 20, the following webinars await you in the field of liquid phase and especially HPLC and LC/MS.</strong></p><blockquote><p><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinars"><strong>👉 DETAILS AND REGISTRATION IN THE WEBINARS SECTION</strong></a></p><p><strong>👉 Search and filter in the largest global database</strong> with over&nbsp;</p><p><strong>4 000 GC, LC, MS and spectroscopy webinars</strong>.</p></blockquote><h5><strong>1. Thermo Fisher Scientific: Characterization and sequence mapping of large RNA and mRNA therapeutics using HRAM</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4580">Registration</a></div></li></ul></div><p>The presentation showcases the innovative partial T1 digest strategy developed at the University of Sheffield for sequencing mRNA therapeutics.</p></div><h5><strong>2.&nbsp;SelectScience/Agilent Technologies: From Start to Finish: How Agilent Can Support Better Decisions in IVT mRNA-Based Biopharmaceutical Analysis</strong></h5><div><div><ul><li><div>Tu, 21.1.2025 17:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4674">Registration</a></div></li></ul></div><p>We will discuss how automated electrophoresis and LC/MS systems solutions from Agilent provide reliable insights for IVT mRNA workflows at many steps.</p></div><h5><strong>3.&nbsp;Phenomenex: LC Troubleshooting Quick Guide: Spot Problems Fast &amp; Fix Them Like a Pro!</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4676">Registration</a></div></li></ul></div><p>In this webinar - General troubleshooting approach, HPLC troubleshooting steps and diagnosing tips and preventive maintenance and column care.</p></div><h5><strong>4.&nbsp;Thermo Fisher Scientific: State-of-the-art E&amp;L is not rocket science</strong></h5><div><div><ul><li><div>Th, 23.1.2025 11:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4581">Registration</a></div></li></ul></div><p>Ulli Honester, a team leader from Solvias, presented on advancements in mass spectrometry for identifying extractables and leachables (E&amp;L).</p></div><h5><strong>5.&nbsp;Waters Corporation: Empower Tips: Ask Me Anything About Navigating Data Governance - Risk and Vigilant Monitoring</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4550">Registration</a></div></li></ul></div><p>We will explore critical aspects of data governance and critical thinking, insights and strategies to help navigate the current environment, evolving your approach and modernizing your way of working.</p></div><h5><strong>6.&nbsp;Evosep Biosystems: A beginner’s guide to Evosep 2025</strong></h5><div><div><ul><li><div>Th, 23.1.2025 16:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4548">Registration</a></div></li></ul></div><p>This webinar is your opportunity to gain a comprehensive understanding of the Evosep One’s key features and how it can seamlessly integrate into your current processes.</p></div><h5><strong>7.&nbsp;Shimadzu Corporation: ASTM D8421 and D8535: Cost Effective Methods for Rapid PFAS Testing in Wastewater and Soil</strong></h5><div><div><ul><li><div>Th, 23.1.2025 20:00 CET</div></li><li><div><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/webinar/4465">Registration</a></div></li></ul></div><p>Discover simplified standardized methods for PFAS analysis based on co-solvation as sample preparation.</p></div>

Webinars LabRulezLCMS Week 4/2025

Article

Webinars

Application

Scientific article

Various

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Agilent Technologies

SelectScience

Shimadzu Corporation

SCIEX

<p><strong>This online event has been designed for our customers and brings together opinion leaders, top scientists and industry innovators to share their research in the biopharma field.</strong></p>
<p>The technologies presented will be CE and LC-MS.</p>
<p>Agenda:</p>
<h5><strong>10:00–10:10 Welcome and introduction</strong></h5>
<ul>
<li>Steve Lock, SCIEX</li>
</ul>
<h5><strong>10:10–10:35 Use of LC-MS in process analytical testing as part of development of a continuous manufacturing workflow</strong></h5>
<ul>
<li>Daniel Myatt / Ronan Gough, CPI</li>
</ul>
<h5><strong>10:35–11:00 Tips and tricks for biotherapeutics characterization by peptide mapping</strong></h5>
<ul>
<li>Giulia Calloni, SCIEX</li>
</ul>
<h5><strong>11:00–11:25 Developing methods for SEC-MS and IEX-MS for the analysis of biotherapeutics</strong></h5>
<ul>
<li>Sarah Nguyen, Strasbourg University</li>
</ul>
<h5><strong>11:25–11:35</strong> Break</h5>
<h5><strong>11:35–12:00 The power of CZE analysis for characterization of NANOBODY® molecules</strong></h5>
<ul>
<li>Dries Dejaegere, Sanofi</li>
</ul>
<h5><strong>12:00–12:25 Multi-capillary gel electrophoresis to increase throughput for the purity analysis of biotherapeutic proteins</strong></h5>
<ul>
<li>Theresa Merlino, The Janssen Pharmaceutical Companies of Johnson &amp; Johnson</li>
</ul>
<h5><strong>12:25–12:50 A disruptive approach to the characterization of mAb charge variants during biopharmaceutical process development</strong></h5>
<ul>
<li>Greg Adams, FUJIFILM</li>
</ul>
<h5><strong>12:50–13:00 Wrap-up and Q&amp;A</strong></h5>
<blockquote>
<p><strong>Presenter:</strong> Steve Lock, PhD (Snr. Marketing and Market Development Manager, Biopharma, EMEAI, SCIEX)</p>
</blockquote>
<blockquote>
<p>Steve obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. He has worked for SCIEX in various technical roles for over 20 years and has presented at over 80 international meetings around the world. He currently works closely with customers in the biopharmaceutical industry. Steve is also currently an Adjunct Professor at the University of Pannonia.</p>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SCIEX_agenda_day_1_6818ee0331/SCIEX-agenda-day-1.jpg" alt="SCIEX - agenda day 1.jpg"/></p>


SCIEX: SCIEX Biopharma meeting - 3rd edition! Day 1 - Gene therapy, vaccines and oligonucleotides

SCIEX Biopharma meeting (Day 1) - Gene therapy, vaccines and oligonucleotides

Gene therapy, vaccines and oligonucleotides. The technologies presented will be CE and LC-MS.

Day 1 - Gene therapy, vaccines and oligonucleotides

<p><strong>This online event has been designed for our customers and brings  together opinion leaders, top scientists and industry innovators to share their research in the biopharma field.</strong></p>
<p>The technologies presented will be CE and LC-MS.</p>
<p>Agenda:</p>
<h5><strong>10:00–10:10 Welcome and introduction</strong></h5>
<ul>
<li>Steve Lock, SCIEX</li>
</ul>
<h5><strong>10:10–10:35 Particle seizing by CE</strong></h5>
<ul>
<li>Hervé Cottet, Montpellier University</li>
</ul>
<h5><strong>10:35–11:00 Application of CE for purity analysis in cell and gene therapy</strong></h5>
<ul>
<li>Ryan Hylands / Luiza Chrojan, Pharmaron</li>
</ul>
<h5><strong>11:00–11:25 Tips and tricks for success in CE analysis</strong></h5>
<ul>
<li>Whitney Smith, SCIEX</li>
</ul>
<h5><strong>11:25–11:35</strong> Break</h5>
<h5><strong>11:35–12:00 RNA payload analysis: development and analytical challenges</strong></h5>
<ul>
<li>Jérémie Parot, SINTEF Industry</li>
</ul>
<h5><strong>12:00–12:25 The use of CE for AAV analytics</strong></h5>
<ul>
<li>Padraig Flaherty, MeiraGTx</li>
</ul>
<h5><strong>12:25–12:50 Oligo analysis by LC-MS</strong></h5>
<ul>
<li>Alexandra Bushby, Labcorp</li>
</ul>
<h5><strong>12:50–13:00 Wrap-up and Q&amp;A</strong></h5>
<blockquote>
<p><strong>Presenter:</strong> Steve Lock, PhD (Snr. Marketing and Market Development Manager, Biopharma, EMEAI, SCIEX)</p>
</blockquote>
<blockquote>
<p>Steve obtained his PhD in Physical Organic Chemistry from the University College of Swansea in 1993 and is a chartered chemist and a member of the Royal Society of Chemistry. He has worked for SCIEX in various technical roles for over 20 years and has presented at over 80 international meetings around the world. He currently works closely with customers in the biopharmaceutical industry. Steve is also currently an Adjunct Professor at the University of Pannonia.</p>
</blockquote>
<p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SCIEX_agenda_day_2_b442145213/SCIEX-agenda-day-2.jpg" alt="SCIEX - agenda day 2.jpg"/></p>


SCIEX: SCIEX Biopharma meeting - 3rd edition! Day 2 - Therapeutic proteins and peptides

SCIEX Biopharma meeting (Day 2) - Therapeutic proteins and peptides

Therapeutic proteins and peptides. The technologies presented will be CE and LC-MS.

Day 2 - Therapeutic proteins and peptides

<p><strong>Parallel capillary electrophoresis is a powerful technique for automated protein analysis. The ProteoAnalyzer system is a novel instrument that uses this technique to overcome the limitations of traditional methods. It features 12 capillaries, validated reagents, and automated cleaning and rejuvenation after each run, enabling the user to obtain high-quality data for various protein sample types.</strong></p>
<p>In this webinar, Dr. Kyle Luttgeharm, Product Manager and Dr. Gizem Bergdahl, Research Scientist, DAKO Denmark R&amp;D &amp; Manufacturing, will discuss how the ProteoAnalyzer system can transform protein analysis workflows.</p>
<p><strong>Key learning objectives</strong></p>
<ul>
<li>Understand more about the ProteoAnalyzer</li>
<li>Learn how to improve the consistency of your protein analysis results</li>
<li>Explore how the ProteoAnalyzer can be used to analyze challenging sample types including total protein extracts and crude cell lysates</li>
</ul>
<p><strong>Who should attend?</strong></p>
<ul>
<li>Anyone who needs help to gain laboratory efficiency, free up technician time, and minimize delays due to ambiguous results leading to re-sample analysis.</li>
</ul>
<p><strong>Certificate of attendance</strong></p>
<p>All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</p>
<blockquote>
<p><strong>Presenter</strong>: Dr. Kyle Luttgeharm (Product Manager, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Dr. Kyle Luttgeharm is a Product Manager with Agilent Technologies. He holds undergraduate degrees in Biology and Biochemistry from Southwestern College in Winfield, Kansas, and a doctorate in Biochemistry from the University of Nebraska-Lincoln. Dr. Luttgeharm previously worked as an Application Scientist with Advanced Analytical Technologies, Inc. and as an R&amp;D Scientist with Agilent Technologies. He has extensive experience developing methodologies and assays for Agilent’s parallel capillary electrophoresis technology.</p>
</blockquote>
<blockquote>
<p><strong>Presenter</strong>: Dr. Gizem Bergdahl (Research Scientist, DAKO Denmark R&amp;D &amp; Manufacturing, Agilent Technologies, (Glostrup, Denmark)</p>
</blockquote>
<blockquote>
<p>Dr. Gizem Bergdahl has a deep understanding of diagnostics, clinical biochemistry, human diseases, and in vitro diagnostics, and extensive experience in areas including immunoassays (ELISA, immunoturbidimetry, and chemiluminescence), ligand-binding assays (SPR), antibody conjugation to latex, and magnetic beads.</p>
</blockquote>
<blockquote>
<p><strong>Presenter</strong>: Blake Forman (Scientific Content Creator, SelectScience)</p>
</blockquote>
<blockquote>
<p>Blake studied chemistry at the University of Surrey, UK, and further went on to study a master’s in chemistry at the University of Southampton, UK. Blake has previously worked in the Medicines and Healthcare Products Regulatory Agency (MHRA) lab at LGC and as a technical sales specialist for Waters. As a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.</p>
</blockquote>


Agilent: Improve laboratory efficiency with automated protein analysis

Improve laboratory efficiency with automated protein analysis

In this webinar, will be discussed how the ProteoAnalyzer system can transform protein analysis workflows.

Improve lab efficiency with automated protein analysis

<p><strong>Reliable and robust analysis is essential throughout in vitro transcription (IVT) mRNA workflows, providing important quality information to help minimize risk and aid in the production of a consistent product. In this webinar, our expert speakers will discuss how the Agilent automated electrophoresis systems are ideal for this assessment, providing fragment sizing and mRNA integrity at a variety of checkpoints throughout the process.</strong></p>
<h5><strong>Key learning objectives</strong></h5>
<ul>
<li>Learn about the quality control (QC) steps that can be performed by the Fragment Analyzer and how they can improve the IVT process, including:<ul>
<li>Quality and size of the linearized plasmid size</li>
<li>Purity analysis of the IVT mRNA</li>
</ul>
</li>
</ul>
<p><strong>Who should attend?</strong></p>
<p>Researchers, lab managers, lab directors, QA/QC managers doing IVT mRNA analysis in biotherapeutics research and production.</p>
<p><strong>Certificate of attendance</strong></p>
<p>All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.</p>
<blockquote>
<p><strong>Presenter:</strong> Whitney Pike (Application Scientist, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Whitney Pike joined Agilent in 2016 and is an application scientist with a focus on Agilent’s automated electrophoresis instruments. She played a pivotal role in the release of the Femto Pulse system as a scientist on the product development team. She completed her master&#39;s degree in Biology, with an emphasis on cellular, molecular, and developmental biology, before working at a core facility at Iowa State University, responsible for performing next-generation sequencing studies.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Leslie Friedmann (Product Manager Parallel CE systems, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Dr. Leslie Friedmann joined Agilent in 2017 as a Product Manager for the Automated Electrophoresis portfolio. She focused first on the ScreenTape portfolio and is now responsible for the parallel CE instruments. Her education includes a diploma in biology from the University of Freiburg, Germany, a PhD in biology from the University of Frankfurt, Germany and a post-doctoral fellowship at the University of Singapore in developmental biology using molecular biology and gene expression analysis techniques.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Professor Harris Makatsoris (Professor Of Manufacturing Systems in the Department of Engineering, King’s College London)</p>
</blockquote>
<blockquote>
<p>Harris is Professor Of Manufacturing Systems in the Department of Engineering at King’s College London and the founder of Centillion Technology Limited, a UK based biotech specialising in the rapid scaling and manufacture of RNA-based therapeutics and vaccines. He has a process engineering background with 27-years experience in both industry and academia. In all his past and present roles, he is managing interdisciplinary projects that require integration and coordination. Harris is an expert in continuous RNA manufacturing. His work is focusing on addressing the challenges in the rapid design, development and scalable manufacture of RNA and other nucleic acids and has pioneered the application of flow technologies in RNA manufacture, of various types. His research has attracted over £26m and he is currently leading a unique multi-disciplinary, multi-million pound programme comprising both industry and academia, building the Biofoundry-in-a-Box (BiaB). The BiaB is a unique portable, multi purpose RNA microfactory designed for the rapid design and deployment of a range of therapeutics and vaccines, with Low and Middle Income Countries in mind. He has several patents in his name in the space of RNA manufacture and continuous bioprocessing. He is highly experienced in technology transfer to industry through a variety of commercial routes.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Jemima Arnold (Editorial Assistant, SelectScience)</p>
</blockquote>
<blockquote>
<p>Jemima studied Cellular and Molecular Medicine at the University of Bristol, UK, and as a member of the Editorial team, she now plays an integral role in shaping the content on SelectScience.</p>
</blockquote>


SelectScience: Best Practices for Analysis of IVT mRNA Integrity using the Agilent Fragment Analyzer Systems

Best Practices for Analysis of IVT mRNA Integrity using the Agilent Fragment Analyzer Systems

Speakers will discuss how the Agilent automated electrophoresis systems are ideal for assessment of in vitro transcription (IVT) mRNA workflows.

Analysis of IVT mRNA using the Agilent Fragment Analyzer

<p><strong>Join us as we delve into the essential principles of Capillary Electrophoresis (CE) and explore its diverse applications across various scientific disciplines. Our industry expert Nandakumar Sundaramoorthy, Ph.D. will share valuable insights and practical knowledge to enhance your understanding of CE.</strong></p>
<h5><strong>Discussion points:</strong></h5>
<ul>
<li>CE and CE-MS: general theory, instrumentation and it&#39;s classification, applications and therapeutic areas</li>
<li>Significance of CE in the world of biotherapeutics and other bio-related products</li>
<li>Breakthrough of CE-based separation in novel biologics</li>
<li>Case study: CE-SDS and cIEF/CZE related problems, root cause and solutions</li>
<li>Quality control, regulatory expectations, and pharmacopeial evolution for CE-based methods</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Nandakumar Sundaramoorthy</p>
</blockquote>
<blockquote>
<p>He is a scientific professional with nearly two decades of global experience, holding a Ph.D. in Applied Proteomics. He is currently the Director - Analytical Development and Characterization at Kemwell Biopharma Pvt Ltd. He has a proven track record of successfully leading end-to-end projects, specializing in protein characterization.</p>
</blockquote>


SCIEX: Fundamentals of capillary electrophoresis (CE)

Fundamentals of capillary electrophoresis (CE)

Seznamte se s námi se základními principy kapilární elektroforézy (CE) a prozkoumejte její rozmanité aplikace v různých vědních oborech.

<p><strong>We want to Help you gain the advantages from advanced automated electrophoresis solutions. Solutions that you’ll need to accelerate your next-generation sequencing projects, cell-free DNA and genomic DNA analysis, RNA and proteins quality controls.</strong></p>
<p>Does your lab need a Tech Upgrade? A technology upgrade may help you save time and money. During the workshop, you’ll hear the benefits your lab can get if you upgrade.</p>
<p><strong>Come to the Workshop to:</strong></p>
<ul>
<li>Learn how the Advanced AE platforms perform in terms of hardware, software, and assays as well as workflows.</li>
<li>See equivalent data between the Bioanalyzer, TapeStation, and Fragment Analyzer system.</li>
<li>Discover the key advantages and benefits of the advanced platforms.</li>
<li>Listen to a customer experience.</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Dr. Matthias Meister (Product Manager, Agilent Technologies Germany)</p>
</blockquote>
<blockquote>
<p>Matthias Meister is Product Manager at Agilent for the Bioanalyzer portfolio. He joined the marketing team for Automated Electrophoresis in 2022 and is based in Waldbronn, Germany. Prior to joining Agilent, he worked as a postdoctoral researcher at the German Cancer Research Center (DKFZ) in Heidelberg, where he also received his PhD.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Sébastien Boulanger (Scientific Expert, Institute of Pathology and Genetics (IPG), Belgium)</p>
</blockquote>
<blockquote>
<p>Sébastien Boulanger integrated the Institute of Pathology and Genetics (IPG) of Gosselies as a lab technician in the molecular biology department in 1999. IPG is one of the eight Genetics Centers recognized in Belgium.</p>
</blockquote>
<blockquote>
<p>He gradually moved to a more scientific position and currently recognized by the Belgian society of Human Genetics as a Medical Genetics Laboratory Expert being now one of the main responsible of the technical lab development.</p>
</blockquote>


Agilent: Upgrade your Tech with Agilent Advanced Automated Electrophoresis Solutions

Upgrade your Tech with Agilent Advanced Automated Electrophoresis Solutions

We want to help you gain the advantages from advanced automated electrophoresis solutions.

Agilent Advanced Automated Electrophoresis Solutions

<p><strong>Explore the intricacies of the world through the lens of nucleic acids—the building blocks of life. Nucleic acids, including the DNA and RNA, play pivotal roles across diverse fields, such as the following examples:</strong></p>
<ul>
<li><strong>Biopharmaceuticals:</strong> The development of mRNA vaccines for COVID-19 highlights the rapid advancements and powerful potential of nucleic acid therapeutics.</li>
<li><strong>Food safety:</strong> Nucleic acid-based detection methods can act as an effective tool to identify foodborne viruses, detect food allergens, and even uncover instances of food fraud.</li>
<li><strong>Forensics:</strong> DNA analysis can shed new insights into crime scene investigations.</li>
<li><strong>Medical diagnostics:</strong> Nucleic acids can be used to detect genetic disorders, cancer biomarkers, and infectious diseases etc.</li>
</ul>
<p>In this Shimadzu Digital Classrooms, join us as we learn more about nucleic acid analysis, using UV-Vis spectroscopy and microchip electrophoresis. We will be introducing Shimadzu’s innovative technologies —TMSPC-8i, BioSpec-nano, and MultiNA—and showcase how they each play a crucial role in various spheres of science and industry. Register now!</p>
<p><strong>Learning Objectives:</strong></p>
<ul>
<li>Learn about the capabilities of Shimadzu&#39;s TMSPC-8i to measure thermal stability of DNA/RNAs</li>
<li>Learn about the convenience of using Shimadzu&#39;s BioSpec-nano for nucleic acid quantitation down to 1µL</li>
<li>Learn how Shimadzu&#39;s MultiNA system can automate gel electrophoresis with improved reproducibility and sensitivity</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Tan Qi An (Application Specialist - Spectroscopy, Shimadzu - Asia Pacific Pte Ltd)</p>
</blockquote>
<blockquote>
<p>Qi An is a highly skilled professional with a bachelor&#39;s degree in Chemistry and Biological Chemistry from Nanyang Technological University, Singapore. His passion for food science led him to pursue a concurrent second major in Food Science and Technology from Wageningen University. With over two years of experience as an Application Specialist for spectroscopy and TOC instruments at Shimadzu Asia Pacific, Qi An has successfully conducted pre-sales demonstrations, sample analysis, and provided application support across diverse industries. His expertise and commitment to delivering high-quality solutions make him a valuable asset to the field of analytical chemistry.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Naatasha Isahak, PhD (Market Specialist - Clinical and Forensics, Shimadzu Asia Pacific)</p>
</blockquote>
<blockquote>
<p>Naatasha Isahak is a Product Specialist for MALDI and Life Sciences Products from Shimadzu Asia Pacific Pte Ltd. She has received her PhD from the University of Sydney (Chemistry) working on surface initiated polymerisation on silica nanoparticles using RAFT polymerisation and bioorthogonal conjugation of peptides using Native Chemical Ligation. She then spent 5 years working on developing platforms for stem cells and organoids characterization that included working with microfluidic devices and helical polymers. Naatasha joined Shimadzu in 2022 to expand her experiences to MALDI characterization on materials and biological samples.</p>
</blockquote>


Shimadzu: Discover the Building Blocks of Life – DNA and RNA Analysis Solutions

Discover the Building Blocks of Life – DNA and RNA Analysis Solutions

Nucleic acid analysis, using UV-Vis spectroscopy and microchip electrophoresis.

DNA and RNA Analysis Solutions

<p><strong>Development and production of IVT mRNA vaccines requires measurements of Critical Quality Attributes (CQAs), such as identity, purity, quality, and quantity. Orthogonal methods developed for such purposes are outlined in the USP guidelines for mRNA vaccines.</strong></p>
<p>Agilent Technologies provides many analytical solutions that are used by leading pharmaceutical companies in early-stage drug development through production quality control (QC) and final product release. These systems help users save time and money with optimized workflows, streamlined processes, and fast time to results.</p>
<p>Whitney Pike, M.Sc, Application Scientist and Dr. Sonja Schneider, Manager Application Development, Agilent Technologies, will discuss how the automated electrophoresis and LC/MS systems solutions from Agilent provide trusted answers for IVT mRNA workflows at many steps, from QC of the initial plasmid DNA, throughout the IVT process, and the finished mRNA drug product.</p>
<h5>Key learning objectives</h5>
<ul>
<li><p><strong>Explore QC throughout the entire IVT mRNA workflow, including data from various nucleic acids and LNP analyses</strong></p>
</li>
<li><p><strong>Discover how Agilent instruments/technologies, such as the Fragment Analyzer and LC/MS systems, can be used for different CQAs</strong></p>
</li>
<li><p><strong>Learn how Agilent is working together with its partners to provide trusted answers for all aspects of the IVT mRNA workflow</strong></p>
</li>
</ul>
<h5>Who should attend?</h5>
<ul>
<li>Researchers, lab managers, lab directors, QA/QC managers performing IVT mRNA analysis in biotherapeutics research and production</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Whitney Pike, MA (Application Scientist, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Whitney Pike joined Agilent in 2016 and is an application scientist with a focus on Agilent’s automated electrophoresis instruments. She played a pivotal role in the release of the Femto Pulse system as a scientist on the product development team. She completed her master&#39;s degree in Biology, with an emphasis on cellular, molecular, and developmental biology, before working at a core facility at Iowa State University, responsible for performing next-generation sequencing studies.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Sonja Schneider (Manager Application Development, Agilent Technologies)</p>
</blockquote>
<blockquote>
<p>Dr. Sonja Schneider joined Agilent in 2011 as an Application Scientist for biopharma applications. She has been leading the Application Development Team since 2016 and still works in the lab on up-to-date biopharma applications. She completed her diploma degree in Biology and her phD in Biochemistry before joining Agilent.</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Will Thompson (Editorial Team, SelectScience)</p>
</blockquote>
<blockquote>
<p>Will studied Biology at the University of Bristol, UK, and as a member of the Editorial team, he now plays an integral role in shaping the content on SelectScience.</p>
</blockquote>


SelectScience: From start to finish: How Agilent Technologies can provide trusted answers for producing IVT mRNA-based vaccines

From start to finish: How Agilent Technologies can provide trusted answers for producing IVT mRNA-based vaccines

How the automated electrophoresis and LC/MS systems solutions from Agilent provide trusted answers for IVT mRNA workflows at many steps

Agilent Technologies in producing IVT mRNA-based vaccines

<p><strong>Hear from experts in capillary electrophoresis (CE) at SCIEX.</strong></p>
<p><strong>In this series, you will learn the fundamentals of the classical applications of CE.</strong></p>
<p>You will understand how to develop methods for protein characterization, RNA/DNA analysis and small molecule detection.</p>
<p>You will discover how these techniques are being used mainly in the biopharmaceutical industry but also in food testing and academia. </p>
<h3><strong>Agenda</strong></h3>
<h5><strong>10:00 Welcome and introduction to SCIEX</strong></h5>
<ul>
<li>Steve Lock, PhD (Senior Market Development Manager, Biopharma, SCIEX)</li>
</ul>
<h5><strong>10:15 Learn how CE-SDS works and how to get the best out of your size mapping of proteins</strong></h5>
<ul>
<li>Marcus Heinl (Senior Field Applications Specialist, SCIEX)</li>
</ul>
<h5><strong>10:45 Understand the differences between cIEF and CZE, and how they both enable charge profiling of proteins</strong></h5>
<ul>
<li>Marcin Moczulski (Senior Specialist Field Applications, SCIEX)</li>
</ul>
<h5><strong>11:15 Find out the latest ways to couple CE with MS detection</strong></h5>
<ul>
<li>Whitney Smith (Senior Support Specialist CE, SCIEX)</li>
</ul>
<h5><strong>11:45 Break / Q&amp;A</strong></h5>
<h5><strong>12:00 Discover how CE can be used for N Glycan analysis</strong></h5>
<ul>
<li>Steve Lock, PhD (Senior Market Development Manager, Biopharma, SCIEX)</li>
</ul>
<h5><strong>12:30 Learn how CE is being used for RNA and DNA analysis and is enabling the development of the latest cell and gene therapy drugs and vaccines</strong></h5>
<ul>
<li>Suki Klair (Field Application Specialist, SCIEX)</li>
</ul>
<h5><strong>13:00 Close / Q&amp;A</strong></h5>
<h5><strong>13:15 End of sessions</strong></h5>


SCIEX: Understanding capillary electrophoresis

Understanding capillary electrophoresis

In this series, you will learn the fundamentals of the classical applications of CE.

<p><strong>Next-generation sequencing (NGS) technologies enable deeper insights into different areas of research. In this new webinar series, researchers from Psomagen, Decode Health, and Agilent Technologies discuss how quality control steps in PacBio Hi Fi long-read sequencing and RNA sequencing support the discovery of new biomarker candidates for diagnostic and therapeutic applications.</strong></p>
<p>Assessing the quality of genomic DNA (gDNA) is of utmost importance in next-generation sequencing (NGS) workflows. Recently, there has been a growing interest in long-read sequencing, as it offers researchers the capability to analyze genomes more comprehensively and accurately. Traditionally, agarose gel electrophoresis/pulsed field gel electrophoresis (PFGE) has been the preferred quality assessment method for long-read sequencing. However, these techniques have limitations regarding sample usage and longer run times. Conversely, recent technological advancements have introduced instruments like the Agilent Femto Pulse system. These instruments can accurately characterize the size distribution of high molecular weight (HMW) DNA and SMRTbell libraries with faster run times and minimal DNA requirements, typically only picogram amounts. This webinar aims to provide an overview of the applications and key benefits of the Femto Pulse system, particularly its use in PacBio long-read sequencing workflows.</p>
<p><strong>Key learning objectives</strong></p>
<ul>
<li>Explore how to effectively assess the quality of genomic DNA (gDNA) in NGS workflows</li>
<li>Learn how the Agilent Femto Pulse system is used in PacBio long-read sequencing workflows</li>
<li>Find out how to determine the number of moles of a sample to better predict long-read sequencing read length</li>
</ul>
<blockquote>
<p><strong>Presenter:</strong> Abhilasha Cheruku, MS (Product Manager-Genomic Services, Psomagen, Inc., United States)</p>
</blockquote>
<blockquote>
<p><strong>Presenter:</strong> Dr. Leslie Friedmann (Product Manager for Parallel CE instruments, Agilent Technologies, Germany)</p>
</blockquote>


Agilent: Analyzing High Molecular Weight (HMW) DNA Size Distribution for PacBio HiFi Long Read Sequencing Services using Agilent Femto Pulse System

Analyzing High Molecular Weight (HMW) DNA Size Distribution for PacBio HiFi Long Read Sequencing Services using Agilent Femto Pulse System

Applications and key benefits of the Femto Pulse system, particularly its use in PacBio long-read sequencing workflows.

LCMS webinars focusing on CE | LabRulez LCMS