Impact of mobile and stationary phases on siRNA duplex stability in liquid chromatography

Welcome to the Column Coach, a tool to help you with reversed-phase column selectivity. This tool uses the Neue Test to help compare column hydrophobicity and selectivity, based on a set of standard conditions using the same test mixtures.For more details about the Neue Test, please refer to:<ul><li><a target="_blank" rel="noopener noreferrer" href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.200390025/full">Neue, U. D.; Van Tran, K.; Iraneta, P. C.; Alden, B. A. Characterization of HPLC Packings. J. Sep. Sci. 2003, 26, 174-186.</a></li><li><a target="_blank" rel="noopener noreferrer" href="https://www.sciencedirect.com/science/article/pii/S0021967399004343">Neue, U. D.; Alden, B. A.; Walter, T. H. Universal Procedure for the Assessment of the Reproducibility and the Classification of Silica-based Reversed-phase Packings II. Classification of Reversed-phase Packings. J. Chromatogr. A. 1999, 849, 101-116.</a></li></ul><h4>How to read the graph?</h4><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Column_Coach_76473457ee.jpg" alt="Waters Corporation: Column Coach" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Column_Coach_76473457ee.jpg 184w," sizes="100vw" width="1000" height="850">All trademarks are the property of their respective owners. Specifications were obtained from manufacturer's public literature. Waters does not endorse or represent the accuracy or reliability of any manufacturers' information, and presentation of this information does not imply any relationship between Waters and any manufacturer. Your use of this information is at your sole risk. This tool and its contents are provided and distributed on an 'as is' basis without warranties of any kind either express or implied, including without limitation, implied warranties or merchantability of fitness for a particular purpose.<h4>You can search for the right column for your application by:</h4><ul><li>Compound Class - Want to find a column for a specific class of compounds? Select a compound class from the drop down. Next select a specific sub-compound class and the recommended Waters columns for that given sub-compound class will be provided.</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Column_Coach_Search_by_Compound_Class_ac76b423b9.jpg" alt="Waters Corporation: Column Coach - Search by Compound Class" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Column_Coach_Search_by_Compound_Class_ac76b423b9.jpg 245w," sizes="100vw" width="1458" height="666"><ul><li>USP Designation - Our quality mission is to ensure that the Waters' columns you use today are the most reproducible and reliable LC columns available. In this section, you will find all Waters columns that are registered according to classifications prescribed in the United States Pharmacopeia (USP).</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Column_Coach_Search_by_USP_Designation_1a8588c591.jpg" alt="Waters Corporation: Column Coach - Search by USP Designation" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Column_Coach_Search_by_USP_Designation_1a8588c591.jpg 190w," sizes="100vw" width="1464" height="1207"><ul><li>Manufacturer of your Existing Column</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Column_Coach_Search_by_Manufacturer_of_your_Existing_Column_cebd6ae8c0.jpg" alt="Waters Corporation: Column Coach - Search by Manufacturer of your Existing Column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Column_Coach_Search_by_Manufacturer_of_your_Existing_Column_cebd6ae8c0.jpg 210w," sizes="100vw" width="1446" height="1073"><ul><li>Selectivity Chart</li></ul><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/Waters_Corporation_Column_Coach_Search_by_Selectivity_Chart_5883ba13ab.jpg" alt="Waters Corporation: Column Coach - Search by Selectivity Chart" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_Waters_Corporation_Column_Coach_Search_by_Selectivity_Chart_5883ba13ab.jpg 245w," sizes="100vw" width="1478" height="904">

Waters HPLC Column Coach

Article

Product

Events

Webinars

Application

Academy

Video

Poster

Scientists

Plastics

Environment

Food

Water

Health

Presentation

Scientific article

Interview

Toxicology

Popularization

Science and research

Software

Covid-19

Career

Laboratories

Various

Zaměření

Type of News

Focus

Everything from the world of analytical chemistry in one place. We connect people in solving their problems. At Labrulez you will find all the necessary information easily, quickly and clearly. Stop searching and start finding.

LabRulez

Waters Corporation, the world's leading specialty measurement company, has pioneered chromatography, mass spectrometry and thermal analysis innovations serving the life, materials and food sciences for nearly 60 years.

Waters HPLC Column Coach

Impact of mobile and stationary phases on siRNA duplex stability in liquid chromatography

Upcoming events

Understanding quantification algorithms

36th MassSpec Forum Vienna 2026

Analytical Strategies for Peptide & Protein Therapeutics

Webinars

High-throughput stability screening strategies for antibody formulation

Big Scale, Big Wins: Prep Chromatography Explained

PFAS in Food & Food Contact Material