Waters 474 Scanning Fluorescence Detector (Refurbished)

The Waters 474 Scanning Fluorescence Detector is analytical instrument designed to provide precise and sensitive spectrofluorometric analysis. Leveraging advanced optical and microprocessor technologies, it enables the detection of low-concentration analytes through fluorescence measurement. The detector’s versatility and reliability make it suitable for applications ranging from trace metal detection to the analysis of organic and biological compounds. The system integrates seamlessly with data management systems for efficient operation and analysis, offering critical features such as excitation and emission wavelength scanning, a second-order filter, and digital signal processing for enhanced performance.

The Waters 474 detector employs a 150 W Xenon lamp as a light source, providing a broad spectrum of high-intensity light in the ultraviolet and visible ranges. With its energy-efficient optics, microprocessor-controlled signal processing, and sophisticated design, the detector ensures accurate and stable fluorescence measurements. Its applications span clinical diagnostics, environmental analysis, and pharmaceutical research.

Technical Specifications

General Specifications:

• Light Source: 150 W Xenon arc lamp
• Detector Type: Photomultiplier tube with nine-stage amplification
• Flow Cell Volume: 16 μL (standard); 5 μL (optional)
• Maximum Pressure: 145 psi (16 μL cell); 500 psi (5 μL cell)
• Wavelength Range:
  • Excitation: 200–600 nm
  • Emission: 210–600 nm
• Sensitivity: Capable of detecting fluorescence from nanogram to picogram levels

Optical Features:

• Excitation and Emission Monochromators: Holographic concave diffraction gratings
• Filters: Optional optical filters for specialized measurements
• Optical Design: Aspherical mirrors and energy-efficient optics to minimize reflection loss

Microprocessor Features:

• Control Interface: Keypad with multi-line liquid crystal display (LCD)
• Signal Processing: Digital control for enhanced operational stability and self-diagnostics
• Auto-Zero Function: Wide range of offset adjustments

Connectivity and Integration:

• Data System Compatibility: RS-232 port for connection to Millennium32 data system
• Analog Outputs: Integrator and recorder output terminals for data collection

Environmental Requirements:

• Operating Temperature: 10–32 °C (50–90 °F)
• Relative Humidity: 20–80%, non-condensing

Power Requirements:

• Input Voltage: 90–132 VAC or 187–264 VAC (selectable)
• Fuse Rating: 5 A (115 V) or 2.5 A (230 V), fast-blow

Dimensions and Weight:

• Dimensions: 44 cm x 28 cm x 18 cm (L x W x H)
• Weight: Approximately 12 kg (26.5 lbs)