Agilent AdvanceBio SEC 300Å HPLC column (4.6 × 300 mm, 2.7 µm) PL1580-5301 (Tested)

The Agilent AdvanceBio SEC 300Å HPLC column (4.6 × 300 mm, 2.7 µm) is specifically designed for size exclusion chromatography (SEC) of proteins and biomolecules. Engineered with fully porous 2.7 µm particles and a 300 Å pore size, this column provides high-resolution separation and reproducibility for aggregate and fragment analysis in biopharmaceutical workflows. It is particularly well-suited for characterizing monoclonal antibodies (mAbs) and protein aggregates across a wide molecular weight range of 5–1,250 kDa.

Constructed with durable stainless-steel hardware, the column supports pressures up to 400 bar and withstands operating temperatures up to 80 °C. Its broad pH tolerance (2–8.5) ensures versatility for diverse applications. The column delivers consistent, reliable performance for advanced protein analysis in biopharma laboratories.

Key Features

• Optimized for protein and mAb aggregate/fragment analysis
• Fully porous 2.7 µm particles with 300 Å pore size for high-resolution SEC
• Broad molecular weight separation range: 5–1,250 kDa
• Stainless-steel hardware rated to 400 bar and 80 °C
• Suitable for LC and LC/MS biopharma workflows

Technical Specifications

• Brand: AdvanceBio SEC
• Hardware: Stainless Steel (SS)
• Inner Diameter (ID): 4.6 mm
• Length: 300 mm
• Particle Size: 2.7 µm
• Particle Type: Fully Porous
• Phase: SEC
• Pore Size: 300 Å
• Pressure Rating: 400 bar
• Maximum Temperature: 80 °C
• Molecular Weight Range: 5,000 – 1,250,000 Da
• pH Range: 2.0 – 8.5
• Separation Mode: Size Exclusion Chromatography (SEC)
• Application Domain: Biopharma
• Typical Applications: 
  • Protein aggregate and fragment analysis 
  • Monoclonal antibodies (mAbs)
  • Proteins
• LC Platform: Stainless Steel
• Technique: LC
• Shipping Solvent: 0.02% Sodium Azide
• UNSPSC Code: 41115711