Introduction to HPLC

Phenomenex Training Courses 2026: Introduction to HPLC

Learn how to set up and run HPLC analysis with a full understanding of all the chromatography theory and method parameters, such as the column, the mobile phase, the instrumentation, and sample preparation. A practical section reporting a detailed discussion about measurement, method and a real case study is also included.
This course is ideal for those who are new to HPLC.

👉Registration

Price (per person)

• £445 | 510 € | 510 CHF | 1200 PLN | 3780 DKK | 5355 NOK | 5620 SEK | 9300 CZK 
• +VAT, incl. PDF of the slides and certificate of attendance

Please note: the seminars take place in two half days (2 sessions of 4 hours each).

Practical Skills Acquired

This course will enable you to implement HPLC analytical methods by a detailed description of HPLC parameters. In addition you will be able to:

• Understand what is meant by all the parameters in an HPLC analytical method.
• Follow an HPLC analytical method to set up an HPLC system for analysis.
• Run an HPLC analytical method and acquire chromatographic results.
• Interpret chromatograms obtained from HPLC analysis.

Course Outline

Instrumentation

• Full system, pump, injector
• Column and column heater
• Detectors overview

Theory

• Mobile phase
• Separation modes
• Columns and stationary phase
• Chromatography theory

Practical Section

• Measurements
• Methods
• Real case study