Troubleshoot HPLC - Identifying, Solving and Avoiding Problems

Phenomenex Training Seminars 2026: Troubleshoot HPLC: Identifying, Solving and Avoiding Problems

Course Summary

Learn how to find solutions to problems encountered when running HPLC analysis by diagnosing symptoms and implementing appropriate preventative measures. This course is ideal for those who have experience using HPLC and now want to develop their skills further.

👉Registration

Price (per person)

• £445 | 510 € | 510 CHF | 1200 PLN | 3780 DKK | 5355 NOK | 5620 SEK | 9300 CZK
• +VAT, incl. PDF of the slides and certificate of attendance

Please note: the seminar takes place in two half days (2 sessions of 4 hours each).

Course Outline

Problem Solving Strategy:

• Leaks
• Variable retention times
• Quantitation and data quality
• Baseline
• System effects

General Chromatographic Problems:

• Assessing the symptoms
• Making the diagnosis
• Finding the appropriate solution
• System problem, preventive maintenance and column care

Peak Problems:

• Fronting and tailing
• Peak split
• Negative and ghost peaks
• Real case study

Practical Skills Acquired

This course will enable you to go back to your lab with a full understanding of why problems may arise with your HPLC system and give you the skills and knowledge to both prevent and resolve those problems. In addition you will be able to:

• Approach and follow the general steps on HPLC troubleshooting.
• Troubleshoot general chromatographic problems, including pressure, leaks, variable retention times, quantitation, data quality and baseline issues.
• Identify the most common peak issues and the possible causes.
• Clean/regenerate the HPLC column.