MSB 2024

MSB 2024: 40th International Symposium on Microscale Separation and Bioanalysis

organized by

Society for Microscale Separations and Bioanalysis

The Society for Microscale Separations and Bioanalysis (SMSB) is committed to promoting the development and applications of microscale separations. Microscale separations enable high-throughput and/or high-performance analyses at reduced costs and with a lower sample and solvent consumption, being therefore highly competitive analytical approaches in today’s research, including fundamental studies, drug discovery and development, clinical and forensic toxicology, food analysis, and -omics approaches.

• REGISTRATION

Speakers

Plenary Speakers

Petra Dittrich (ETH Zurich)

• TBA

Andras Guttman (University of Debrecen, University of Pannonia)

• Analysis of Proteins and Peptides by Native and SDS Capillary Agarose Gel Electrophoresis Online Coupled to Electrospray Ionization Mass Spectrometry

Marek Minárik (Elphogene, s.r.o., Charles University)

• Applications of DNA Capillary Electrophoresis in Molecular Cancer Diagnostics: Above and Beyond Sanger Sequencing

Milos V. Novotny (Indiana University, Department of Chemistry)

• Comprehensive N-Glycan Profiling in Cancer Research: Past, Present and Future

Keynote Speakers

Hyun Joo An (Chungnam National University)

• Comprehensive Characterization of Mammalian Brain N-Glycome: Isomer-Sensitive Nano-LC-MS/MS Analysis and its Application to Alzheimer's Disease Models

Zuzana Bilkova (University of Pardubice)

• When Microfluidics Meets Magnetic Nanomaterials: Recent Approaches in Bioanalysis

Norberto Guzman (Princeton Biochemicals, Inc.)

• The Study of Sputome: A Need for Sputum as a Rich Source of Protein Biomarkers for the Non-Invasive Diagnosis of Infectious and Chronic Diseases

Alexander Ivanov (Northeastern University)

• TBA

Petr Kubáň (Institute of Analytical Chemistry of the Czech Academy of Sciences)

• TBA

Jan Preisler (Masaryk University)

• Imaging of Individual Nanoparticles by Mass Spectrometry

Michael Roper (Department of Chemistry & Biochemistry, Florida State University)

• Bioanalytical Approaches for Monitoring Cellular Communication

Alexandra Ros (Arizona State University)

• TBA

Cari Sänger – van de Griend (Uppsala University, Kantisto BV)

• AQbD, ICH Q14 – Should I Bother and What Does This Mean for My CE Method Development?

Govert Somsen (Vrije Universiteit Amsterdam)

• Multidimensional Assessment of Polymer Nanoparticles

Jan Tkac (Institute of Chemistry, Slovak Academy of Sciences)

• TBA

Hermann Wätzig (University of Braunschweig)

• Biopharmaceuticals by Capillary Electrophoresis: Mass Spectrometry, Affinity, Isoelectric Focusing, Process Analysis

Program Schedule

The final program of oral presentations will be published in mid-March.

Sunday, May 19

Short Courses

Opening Ceremony & Plenary Lectures

Opening Reception

Monday, May 20

Morning Sessions

Lunch Break & Poster Session

Afternoon Sessions

Tuesday, May 21

Morning Sessions

Lunch Break & Poster Session

Visit to the Mendel Museum

Conference Dinner

Wednesday, May 22

Morning Sessions

Lunch Break

Plenary Lectures & Closing Remarks

Session Topics

This symposium offers a unique multidisciplinary touch from microscale engineering to separation sciences and their applications with the following conference topics.

• Advances in microscale CE and LC separations
• New trends in MS and IMS for bioanalysis
• Nanoscale separation systems for interaction studies
• Microscale sample preparation for bioanalysis
• Pharma and biopharma applications
• Biomarkers for precision medicines
• Microscale separations for -omics sciences
• Native protein separation and detection
• Materials for microfluidic/lab-on-chip systems
• Digital microfluidics, droplet microfluidics, centrifugal microfluidics
• Sensor fabrication technologies (3D printing, lithography, etc.)
• Biomarkers and functionalization
• Microfluidic platforms for integrated separation and detection
• Point-of-care devices / from lab-on-chip systems towards applications
• Advances in organ-on-chip engineering

Awards

SCIEX Microscale Separations Innovations Medal and Award

(previously the Arnold O. Beckman Award)

The SCIEX Microscale Separations Innovations Medal and Award (previously the Arnold O. Beckman Award) is an annual award given to an individual for remarkable career achievements, with particular consideration being given to the development of new methods, techniques and high-impact applications in the field of electrodriven separations. The award is supported by SCIEX, a key driver in capillary electrophoresis technology and comprises a medal, a diploma and a monetary prize.

Jaroslav Janák Award

Since 2014, the Jaroslav Janák Award for the Development of Analytical Chemistry has been awarded by the Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic to scientists who have made a significant contribution to the development of analytical chemistry through their work.

Young Scientist Award

The award(s) will be presented to a graduate student(s) or postdoctoral fellow(s) giving an oral presentation at MSB 2024. An international jury of scientists will judge the qualified presentations and choose a winner. If interested, please submit your abstract regularly via the conference website and send the application (abstract, proof of student/postdoctoral status & short recommendation letter from supervisor) by e-mail to [email protected].

Best Poster Award

The award(s) will be presented to a MSB 2024 participant(s) actively presenting a poster during the poster sessions. All presenters are eligible for consideration. An international jury of scientists will judge the qualified presentations and choose a winner.

Short Courses

Half day short courses are offered for an additional fee of EUR 50 / CZK 1250 per person and will be held on Sunday afternoon, May 19, 2024, 1:00 - 4:00 p.m. The minimum number of participants to open each short course is 5.

1. Ion Mobility Spectrometry

• Tim Causon (BOKU - University of Natural Resources and Life Sciences, Vienna, Austria)

Course Description:

Ion mobility (IM) complements existing analytical methods as it involves millisecond-timescale separations of gas phase ions according to their structure and not only their mass. In combination with mass spectrometry, IM-MS is now a powerful technology that can be applied to very diverse analytical applications. This short course will introduce the basics of IM theory, separation principles, and the derivation of collision cross section (CCS) from IM-MS measurements. Important instrumental aspects of IM spectrometry and IM-MS technologies will be introduced, and application examples from diverse application fields (e.g., food, metabolomics, environmental analysis) shown to highlight how ion mobility be applied successfully in analytical methods.

Instructor:

Assoc. Prof. Tim Causon is an Associate Professor at Institute of Analytical Chemistry, University of Natural Resources and Life Sciences, Vienna (Austria). His main areas of research are liquid chromatography, molecular mass spectrometry, and ion mobility-mass spectrometry (IM-MS) addressing diverse analytical method development questions as well as fundamental studies of ionisation and properties of small molecular ions. Current research topics include applications of these analytical techniques for optimization of both upstream (e.g., microbial cell factories) and downstream (e.g., purification) ends of the bioprocessing continuum, fundamental investigations of gas-phase isomers of small molecule systems, and addressing the urgent need for harmonization of IM-MS measurement and reporting standards.Starting in 2024, he will be the co-ordinator of the MSCA Doctoral Network “MobiliTraIN” (Ion Mobility Mass Spectrometry Training Network).

2. Mass Spectrometry Imaging

• Jan Preisler - Antonin Bednarik (Masaryk University, Brno, Czech Republic)

Course Description:

This course provides a comprehensive understanding of mass spectrometry imaging (MSI) principles and techniques. The most common ionization techniques involved in MSI, namely matrix-assisted laser desorption/ionization (MALDI), desorption electrospray (DESI), secondary ion mass spectrometry (SIMS) and laser ablation inductively-coupled plasma (LA ICP), are reviewed. The MALDI technique is emphasized, as it presents the most widespread MSI platform. Sample preparation methods are discussed in detail, including tissue sectioning and storage, washing protocols, matrix application, and on-tissue reactions. Current instrumentation, a common data format (imzML) structure, software tools for data processing and visualization, selected applications, and the latest achievements in MSI are presented.

Instructors:

Prof. Jan Preisler is a professor of analytical chemistry at Masaryk University in Brno, Czech Republic. He received his Ph.D. with Ed Yeung at Iowa State University, Ames, and spent four years in the group of Barry Karger at Barnett Institute, Boston. His research interests include the development of instrumentation and methods for bioanalytical chemistry, MS imaging, time-of-flight mass spectrometers, characterization and analytical applications of nanoparticles, single-particle analysis, and development of new sample introduction techniques for inductively coupled plasma mass spectrometry. He pioneered the use of kHz lasers to increase the throughput of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in imaging applications and to detect parallel column separations.

Antonin Bednarik, Ph.D. is a postdoc researcher at Masaryk University in Brno, Czech Republic. His research activities include the development of MALDI MS and MSI instrumentation, analysis of volatile organic compounds, the development of novel MSI techniques, and applications of MSI in the analysis of nanoparticles and lipidomics. After receiving his Ph.D., he spent one year as a postdoc in the group of Klaus Dreisewerd in Münster, where he developed on-tissue Paternò Büchi derivatization protocol for MALDI MSI of lipid double bond positional isomers. He continues studying on-tissue derivatization reactions for MSI of lipid isomers and their potential in clinical applications.

3. Introduction to Miniaturization and Microfluidics

• Petr Kuban - Jakub Novotny - Tomas Vaclavek - Jana Krivankova (Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic)

Course Description:

The main objective of this short course is to provide the participants with an overview of the basic concepts of miniaturization and microfluidic technology. We will discuss the fundamentals of microfabrication techniques involved in manufacturing of microfluidic devices as well as what are the main benefits of utilizing such devices in analytical chemistry. In a series of lectures the participants will first gain knowledge and skills on two major techniques for fabricating microfluidic devices in glass and polymeric materials. The techniques will include glass chip fabrication techniques, such as photolithography, metal deposition, and etching. The information regarding the fabrication of plastic- and polymer-based systems, such as PDMS chips, will encompass casting and other soft lithography techniques. Eventually there will be a comprehensive lecture on 3D printing, showing 3D printing techniques, such as FDM and SLA, discussing the advantages of various printing materials, economic and sustainability aspects, etc., and showing practical examples of a 3D printed casing for a microfluidic devices. The participants will also gain knowledge on other computer-controlled methods associated with the fabrication of microfluidic devices, such as CNC micromachining. Last but not least the control of microfluidic devices with the use of Arduino microcontroller, basic programming using the IDE and examples of use of Arduino in analytical instruments will be shown. Additionally, specific applications like single-cell analysis and droplet microfluidics, and more will be discussed as a part of this course.

Instructors:

Assoc. Prof. Petr Kuban obtained his doctorate from the Department of Analytical Chemistry, Stockholm University, Sweden and spent three years as a post-doc at the Department of Chemistry and Biochemistry, Texas Tech University, USA. Currently he is the Head of the Department of Bioanalytical Instrumentation at the Institute of Analytical Chemistry of Czech Academy of Sciences in Brno, Czech Republic. His main research interests include micro-column separation techniques such as capillary electrophoresis and HPLC, flow injection analysis, sample pretreatment and preconcentration, miniaturization, microfluidics and development of novel analytical instrumentation for clinical diagnostics.

Jakub Novotny, Ph.D. aimed his Ph.D. studies at the Institute of Analytical Chemistry (IAC) of the CAS in Brno, Czech Republic, towards the applications of microfluidics in biochemistry, with a specific emphasis on microfabrication methods. This involved the development of microfluidic devices using CNC fabrication methods (e.g. micro-milling), 3D printing, and photolithography. During his postdoctoral research in Lund, Sweden, he explored the techniques related to the field of acoustofluidics (study of the interaction of soundwaves with fluids and dispersed particles at the microscale). Now as a research assistant back at the IAC, his work continues to revolve around acoustofluidics and the development of acousto/microfluidic devices.

Tomas Vaclavek, Ph.D. has received his Ph.D. in Biochemistry from the Masaryk University, developing various microfluidic systems focused on single-cell manipulation, non-optical particle detection, intracellular compound isolation with subsequent analysis by mass spectrometry. His collaborations provided valuable experience with miniaturization of biosensing systems or development of microfluidic interfaces for 2D liquid separations. Currently, he develops micromachined nanospray interfaces as ion sources for highly-sensitive bioanalyses.

Jana Krivankova, Ph.D. passed her doctoral studies at the Institute of Analytical Chemistry of the Czech Academy of Sciences in Brno. Since 2016, she explored the research related to the fabrication techniques, focused on development and optimization of droplet-based microfluidic devices. Today’s research deals with application of photon-conversion nanoparticles and automation on microfluidic chips for detection of clinically relevant protein markers.

Committees

Local Organizing Committee

Frantisek FORET, Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic

Jana LAVICKA, Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic

Jan PRIKRYL, Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic

Petr KUBAN, Institute of Analytical Chemistry of the Czech Academy of Sciences, Brno, Czech Republic

Scientific Committee

Zuzana BILKOVA, University of Pardubice, Pardubice, Czech Republic

Doo Soo CHUNG, Seoul National University, Seoul, Korea

Petra DITTRICH, ETH Zürich, Basel, Switzerland

Andras GUTTMAN, University of Debrecen, Debrecen, Hungary

Lisa HOLLAND, West Virginia University, Morgantown, WV, USA

Alexander IVANOV, Northeastern University, Boston, MA, USA

Jörg KUTTER, University of Copenhagen, Copenhagen, Denmark

Christian NEUSUSS, Aalen University, Aalen, Germany

Cari SANGER, Kantisto, Baarn, The Netherlands

Govert W. SOMSEN, Vrije Universiteit Amsterdam, Amsterdam, The Netherlands

Frantisek SVEC, Charles University, Hradec Kralove, Czech Republic

Myriam TAVERNA, Paris-Saclay University, Paris, France

Karen WALDRON, University of Montreal, Montreal, Canada

Susanne WIEDMER, University of Helsinki, Helsinki, Finland

Abstract Instructions

Before abstract submission, register first please: Register

If you are already registered, you can submit abstract: Submit an abstract

See below for more information.

Abstract Submission Deadlines

Oral Presentation – SUBMISSION IS CLOSED

Poster Presentation – until April 30, 2024

Peer Review

Submitted abstracts will be assessed by the MSB 2024 Scientific Committee in a double-blind manner. Reviewers will not have access to your name, institution, or position status. The review will be based solely on the scientific information presented in the abstract.

Four criteria will be used to evaluate each abstract: originality of the work, quality of the abstract/technical description, relevance to the symposium topics, relevance to the field of microscale separations and bioanalysis.

Abstract Requirements

• Researchers with new results in areas relevant to the meeting are encouraged to submit an abstract online for oral or poster presentation consideration.
• One abstract may be submitted per attendee as presenting author.
• The presenting author must be registered as a delegate and pay the required fee by the registration deadline.
• All presenters of an oral presentation who are graduate students or postdoctoral fellows at the end date of the meeting are eligible for Young Scientist Award, depending on availability.
• All abstracts must be written exclusively in English (including the title, abstract text, author names, and affiliations).
• Kindly see links to Key Dates & Topics for more information about topic areas and abstract submission deadlines.
• If you cannot submit the abstract prior to receiving company approval by the date abstracts are due (see Key Dates), please submit a placeholder with the title, authors, and a brief description of the kind of material that you would like to present, and indicate when approval is expected. This material will be kept confidential. It is your responsibility to revise the abstract to the final version upon approval.
• Authors will be notified whether the submitted abstract has been accepted for oral or poster presentation, or was not selected for presentation.

Abstract Submission

• First, please register your participation on the conference (will open on January 1, 2024).
• Use the web form using your unique registration code (received within registration) to submit an abstract.
• It is allowed to submit one abstract per registered participant.
• The summary paragraph of the abstract should be no more than 300 words and contain a summary of the work presented. - Title, Authors, Affiliations, References (optional) and Acknowledgments do not count towards this limit. If you add any graphics, shorten the text so that the total maximum length of the abstract is 1 page.
• Abstract Title: Every word in the abstract title should be capitalized (with the usual exceptions). The use of CAPITAL LETTERS should be limited to abbreviations.
• Include author names, affiliations, or self-references within the abstract; nevertheless, they will be temporarily removed for a double-blind review by Scientific Committee.
• References, if presented, should be numbered sequentially in the order in which they are cited in the text. The numbers should be set in brackets, thus [1, 8]. References are to be collected in numerical order at the end of the abstract under the heading "References". Please format the references according to the Analytica Chimica Acta journal style (see the basic examples below). Titles of journals should be abbreviated.
  • Reference to a journal publication: [1] J. van der Geer, J.A.J. Hanraads, R.A. Lupton, The art of writing a scientific article, J. Sci. Commun. 163 (2010) 51-59.
  • Reference to a book: [2] W. Strunk Jr., E.B. White, The Elements of Style, fourth ed., Longman, New York, 2000.
  • Reference to a chapter in an edited book: [3] G.R. Mettam, L.B. Adams, How to prepare an electronic version of your article, in: B.S. Jones, R.Z. Smith (Eds.), Introduction to the Electronic Age, E-Publishing Inc., New York, 2009, pp. 281-304.
• Please note that it is your responsibility to fill in all data correctly and completely. Any spelling, grammatical or scientific errors will be reproduced as written by the author.
• If your abstract cannot be accepted as an oral presentation, it will be considered for a poster presentation.

Information

Key Dates

Opening Registration and Abstract Submission

• January 8, 2024

Early Bird Registration*

• until March 31, 2024

Standard Registration

• until May 10, 2024

Abstracts – Oral Presentation

• until February 16, 2024

Abstracts – Poster Presentation

• until April 30, 2024

Conference

• May 19 – 22, 2024

• For attendees with active participation, the discount is only available if their abstract is submitted before March 31, 2024!

Please note that the number of participants is limited.

Oral Presenter Information

• There will be several kinds of lectures: Invited (Plenary and Keynote), Contributed and Junior (given by Ph.D. candidates) lectures. Time slots will be specified in final program.
• Please plan to use the computer at the podium (a Windows computer running PowerPoint) and use a 16:9 resolution for your slides.
• There will be breaks before each session, so please arrive in your meeting room on time to check that your presentation is working. You will have a presenter with a laser pointer at your disposal.

Poster Presenter Information

• The preferred poster size is A0 (portrait). Nevertheless, the maximum poster size is 100 x 120 cm vertical (portrait) orientation. The poster does not necessarily have to fill the entire board area.
• The boards and pushpins to mount the poster will be provided.
• Posters will be assigned a number that will be attached to the board; authors should mount their posters only on the assigned board.
• Posters are to be displayed during the whole meeting.
• Poster prensenters are asked to be available during the poster session according to the conference program

Registration Fees

General

• Early Bird, until March 31, 2024 - EUR 450 / CZK 11,000
• Regular, until May 10, 2024 - EUR 550 / CZK 13,500
• On-site - EUR 650 / CZK 16,000

Student

• Early Bird, until March 31, 2024 - EUR 300 / CZK 7,500
• Regular, until May 10, 2024 - EUR 350 / CZK 8,600
• On-site - EUR 400 / CZK 10,000

The price of a one-day registration is EUR 200. For one-day registration, please contact us by email.

The registration fee includes admission to all lecture and poster sessions, the conference program, coffee breaks and lunches (Monday, Tuesday, Wednesday), the banquet and visit to Mendel Museum, but does not include the accommodation.

Short Courses

Half day short courses are offered for an additional fee of EUR 50 / CZK 1250 per person. The courses will be held on Sunday afternoon, May 19, 2024, 1:00 - 4:00 p.m. simultaneously. The minimum number of participants to open each short course is 5.

Venue

Hotel Continental Brno

Address: Kounicova 680/6, 602 00 Brno-střed-Veveří, Google maps

Wireless Internet connection (Wi-Fi) is available in all public areas of the hotel, including conference areas

Hotel Continental web pages

Conference facilities of Hotel Continental

Accommodation Reservation & Prices

Please book your room directly by email at [email protected] or by phone at +420 541 519 609 with the code “MSB2024”.

Special offer for the conference participants:

The rate of 1980 CZK/night for a single room or 2350 CZK/night for a double room (including VAT and breakfast, without city tax) is available for conference participants.

Please note that the number of rooms is limited and the offer is only valid until May 3, 2024.

The registration fee includes admission to all lecture and poster sessions, the conference program, coffee breaks and lunches (Monday, Tuesday, Wednesday), and the banquet, but does not include accommodation.

Contacts

[email protected]

Institute of Analytical Chemistry of the Czech Academy of Sciences, v. v. i.

Veveří 97, 602 00 Brno, Czech Republic

Vat No: CZ68081715

Tel: +420 532 290 182, Fax: +420 532 290 100, www.iach.cz

Sponsorship & Advertising Opportunities

MSB 2024 is the premier international conference on microscale separations and bioanalysis that spans new advances in technology/method development across the landscape of separation science and analyte detection with cutting-edge applications. The philosophy of the MSB symposia aims at encouraging the presentation and discussion of new and mostly unpublished results. The forum encourages vigorous scientific discussions between participants with the support of committed and engaged session chairs. We anticipate 200+ delegates at MSB 2024, spanning academia, government, and industry.

Putting the Focus on Our Sponsors

Funding by our sponsors at MSB will be recognized on the website, during the conference kick-off, throughout the duration of the conference, and in the Final Program. And we are happy to work with you to develop a customized sponsorship vehicle that puts the spotlight on your organization – tell us what works for your organization!

Funding by our sponsors and supporters at MSB 2024 will be recognized based on their support level, ranging from Sponsor, Bronze, Silver, Gold, to Platinum. No matter the support level, your company/institution will be recognized on the MSB 2024 website, on a slide presentation with the other sponsors throughout the conference, and in the Final Program.

How to Become a Sponsor?

We invite you to become a sponsor and ask you to contact Jana Lavicka (Sponsorship Coordinator, e-mail: [email protected]) to secure your sponsorship while they last.

Thank you for helping to make MSB 2024 a great success!

Yours sincerely, Frantisek Foret (Chair)

Sponsors & Supporters

MSBS 2024: sponsors