HPLC 2024 - 52nd International Symposium on High Performance Liquid Phase Separations and Related Techniques.
Delve into the multifaceted world of separation sciences in both liquid and supercritical fluid phases. Discover multidimensional chromatography, hyphenated techniques with pioneering HPLC detection technologies, primarily mass spectrometry, and explore the latest in separation materials, column technologies, and cutting-edge complex mixture analyses.
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Our diverse program encompasses:
- Fundamentals: Dive deep into the theory of chromatographic separations and detection principles.
- Technological Advances: Stay updated on liquid chromatography developments and innovations.
- Applications & QA: Explore HPLC applications across diverse sectors and quality assurance.
This symposium promises engaging chromatography workshops, tutorials, and sessions from chromatography experts and luminaries in separation sciences. Moreover, our expansive exhibition and vendor seminars, featuring analytical instrument suppliers, present the newest breakthroughs from industry leaders.
Don't miss the Denver conference that brings together the best in analytical chemistry at this international scientific symposium. Join us for an enriching experience in liquid chromatography and mass spectrometry innovations.
HPLC 2024: 52nd International Symposium on High Performance Liquid Phase Separations and Related Techniques
Key Dates
Mark your calendars and make sure you don't miss out on these crucial dates for the HPLC 2024 conference in Denver, Colorado.
Stay informed about important deadlines and milestones for HPLC 2024 Mark your calendars and make sure you don't miss out on these crucial dates. Stay tuned for updates and additional dates as they become available. We look forward to welcoming you to HPLC 2024!
FRIDAY, DECEMBER 15, 2023: Abstract Submission Portal Opens
MONDAY, JANUARY 8, 2024: Start Registration
MONDAY, MARCH 18, 2024: Abstract Deadline for Oral Presentations
MONDAY, APRIL 22, 2024: Preliminary Program Published Online
MONDAY, MAY 6, 2024: Deadline for Poster Abstract Submissions
MONDAY, MAY 20, 2024: Deadline for Early-bird Registration Fees
MONDAY, JUNE 3, 2024: Deadline for Reduced Registration Fee
MONDAY, JUNE 3, 2024: Registration and Payment Deadline for All Presenting Authors
MONDAY, JULY 1, 2024: Scientific Program Published Online
SATURDAY, JULY 20, 2024: Start of HPLC 2024 in Denver
Registration
Registration Fees By May 20
- Industrial Bundled = 1,450 USD
- Industrial Full = 1,375 USD
- Academic / Government Bundled = 995 USD
- Academic / Government Full = 890 USD
- Student / Post-Doc Bundled = 299 USD
- Student / Post-Doc Full = 199 USD
- Full-Day Short Course w. registration = 200 USD
- Half-Day Short Course w. registration =100 USD
- Full-Day Short Course no registration = 400 USD
- Half-Day Short Course no registration = 200 USD
Registration Fees By July 1
- Industrial Bundled = 1,550 USD
- Industrial Full = 1,475 USD
- Academic / Government Bundled = 1,095 USD
- Academic / Government Full = 990 USD
- Student / Post-Doc Bundled = 299 USD
- Student / Post-Doc Full = 199 USD
- Full-Day Short Course w. registration = 200 USD
- Half-Day Short Course w. registration =100 USD
- Full-Day Short Course no registration = 500 USD
- Half-Day Short Course no registration = 250 USD
Registration Fees After July 1 & Onsite
- Industrial Bundled = 1,650 USD
- Industrial Full = 1,575 USD
- Academic / Government Bundled = 1,250 USD
- Academic / Government Full = 1,090 USD
- Student / Post-Doc Bundled = 339 USD
- Student / Post-Doc Full = 239 USD
- Full-Day Short Course w. registration = 300 USD
- Half-Day Short Course w. registration =150 USD
- Full-Day Short Course no registration = 600 USD
- Half-Day Short Course no registration = 300 USD
*Bundled package includes 1 meeting registration, 1 gala dinner ticket, 1 half-day short course (If full-day short course is preferred, purchase an additional half-day short course has to follow).
*Full registrations include only 1 meeting registration. Short courses and Gala dinner tickets must be purchased separately.
Cancellation Policy
All cancellations must be made before July 1, 2024. No refunds will be made after this date. There will be an administrative fee of US$150 deducted from each refund. You can modify or cancel your registration using the “Already Registered?” link on the registration site.
Program
Program-at-a-Glance
*Tentative schedule - subject to change
Saturday, July 20 (Short Courses)
7:00 Registration Opens
8:00-9:30
- Two-dimensional Liquid Chromatography: Principles, Instrumentation, Method Development, and Applications (Colorado G)
- Practical LC-MS/MS method development (Colorado H)
- Analysis of Oligonucleotide Therapeutics (Colorado I)
9:30-10:00 Coffee Break (Pre-function Area)
10:00-11:30
- Two-dimensional Liquid Chromatography: Principles, Instrumentation, Method Development, and Applications (Colorado G)
- Practical LC-MS/MS method development (Colorado H)
- Analysis of Oligonucleotide Therapeutics (Colorado I)
11:30-13:00 Lunch on your own
13:00-14:30
- Two-dimensional Liquid Chromatography: Principles, Instrumentation, Method Development, and Applications (Colorado G)
- Practical LC-MS/MS method development (Colorado H)
- Analysis of Oligonucleotide Therapeutics (Colorado I)
Sunday, July 21 (Short Courses)
7:00 Registration Opens
8:00-9:30
- (u)HPLC Method Development (Colorado G)
- FFF/SEC with light scattering detection (Colorado H)
- Chiral Liquid Chromatography (Colorado I)
- Supercritical Fluid Chromatography (Colorado J)
9:30-10:00 Coffee Break (Pre-function Area)
10:00-11:30
- (u)HPLC Method Development (Colorado G)
- FFF/SEC with light scattering detection (Colorado H)
- Chiral Liquid Chromatography (Colorado I)
- Supercritical Fluid Chromatography (Colorado J)
11:30-13:00 Lunch on your own
13:00-14:30
- Capillary Electrophoresis with Mass Spectrometry Detection (Denver 1)
- HPLC Operation, Maintenance and Troubleshooting (Denver 2)
- Learning Chromatography in Chromatograms (Denver 3)
- Separation of Glycans and Glycopeptides derived from Glycoproteins (Denver 4)
- Capillary Liquid Chromatography (Denver 5)
- Accurate Liquid Chromatography Solutions for Cannabinoids Quantitation (Denver 6)
14:30-15:00 Coffee Break (Pre-Function Area)
15:00-16:30
- Capillary Electrophoresis with Mass Spectrometry Detection (Denver 1)
- HPLC Operation, Maintenance and Troubleshooting (Denver 2)
- Learning Chromatography in Chromatograms (Denver 3)
- Separation of Glycans and Glycopeptides derived from Glycoproteins (Denver 4)
- Capillary Liquid Chromatography (Denver 5)
- Accurate Liquid Chromatography Solutions for Cannabinoids Quantitation (Denver 6)
16:30-17:00 Opening Ceremony (Colorado F-J)
17:00-17:40 Plenary 1 - Steven Soper Colorado F-J
17:40-18:20 Plenary 2 - Janusz Pawliszyn (Colorado F-J)
18:20-20:20 Welcome Reception & Toast at Plaza (Pre-function are backup)
Monday, July 22
7:00 Registration Opens
8:30-8:40 Opening Session (Colorado F-J)
8:40-9:20 Plenary 3 - Claus Rentel (Colorado F-J)
9:20-10:00 Plenary 4 - Alexandra Ros (Colorado F-J)
10:00-10:30 Coffee Break (Pre-Function Area)
10:30-11:30 Parallel Scientific Session (Denver 1-6, Colorado G-J)
11:30-12:30 Poster Session & Exhibition (Colorado A-F)
12:30-13:30 Vendor Lunch Workshops, Lunch on your own (Denver 1-6, Colorado G-J)
13:30-15:00 Parallel Scientific Session (Denver 1-6, Colorado G-J)
15:00-16:30 Poster Session, Exhibition, & Coffee Break (Colorado A-F)
16:30-18:00 Parallel Scientific Session (Denver 1-6, Colorado G-J)
18:00-20:00 HPLC Tube + Reception (Denver 1-3 & Pre-Function Area)
Tuesday, July 23
7:30 Registration Opens
8:30-10:00 Parallel Scientific Session (Denver 1-6, Colorado G-J)
10:00-11:00 Poster Session, Exhibition, & Coffee Break (Colorado A-F)
11:00-12:30 Parallel Scientific Session (Denver 1-6, Colorado G-J)
12:30-13:30 Vendor Lunch Workshops, Lunch on your own (Denver 1-6, Colorado G-J)
13:30-15:00 Parallel Scientific Session (Denver 1-6, Colorado G-J)
15:00-16:30 Poster Session, Exhibition, & Coffee Break (Colorado A-F)
16:30-18:00 Parallel Scientific Session (Denver 1-6, Colorado G-J)
Wednesday, July 24
7:30 Registration Opens
8:30-10:00 Parallel Scientific Session (Denver 1-6, Colorado G-J)
10:00-11:00 Poster Session, Exhibition, & Coffee Break (Colorado A-F)
11:00-12:30 Parallel Scientific Session (Denver 1-6, Colorado G-J)
12:30-13:30 Vendor Lunch Workshops, Lunch on your own (Denver 1-6, Colorado G-J)
13:30-15:00 Parallel Scientific Session (Denver 1-6, Colorado G-J)
15:00-16:30 Poster Session, Exhibition, & Coffee Break (Colorado A-F)
16:30-18:00 Parallel Scientific Session (Denver 1-6, Colorado G-J)
17:30-19:00 Cocktail Hour
19:00-22:00 Conference Dinner
- KEVIN TAYLOR’S AT THE OPERA HOUSE (Limited number of tickets available, )urchased in addition to conference registration)
Thursday, July 25
7:30 Registration Opens
8:30-10:00 Parallel Scientific Session (Denver 1-6, Colorado G-J)
10:00-11:00 Poster Session, Exhibition, & Coffee Break (Colorado A-F)
11:00-12:30 Parallel Scientific Session (Denver 1-6, Colorado G-J)
12:30-13:30 Lunch on your own
13:30-16:30 Plenary/Awards/Introduction to Future HPLCs/Closing (Colorado F-J)
16:30-17:30 Farewell Drink at Plaza (Pre-function are backup)
Plenary Speakers
Steven Soper (University of Kansas)
Detection and Identification of Single Molecules and Particles using Resistive Pulse Sensing and Nanoscale Electrophoresis
- Microchip electrophoresis has dramatically expanded the area of bioseparations due to potential benefits it offers, such as the integration of the separation processing step with sample preparation. However, the separation mechanisms for microchip electrophoresis and capillary electrophoresis are, in principle, the same.
Janusz Pawliszyn (University of Waterloo)
Chemical Biopsy SPME Probe, a Green Sample Preparation Alternative for HPLC
- Development of devices to perform chemical determination of organic compounds in complex matrices involves two main directions. One is to design sensors, which are simple portable on-site but typically limited to single component determination with selectivity determined by membrane and/or readout characteristics. The other approach is to use hyphenated separation with mass spectrometry technologies such as GC/MS and LC/MS which allow multicomponent determination typically limited by sample preparation to deliver good analytical performance.
Claus Rentel (Ionis Pharmaceuticals)
Analysis of Oligonucleotide Therapeutics
- Oligonucleotides are a novel class of drugs with the potential to treat a wide spectrum of indications, including cancer, cardiovascular and metabolic conditions, neurological disorders, pulmonary and ophthalmic diseases. Several different modalities using oligonucleotides to treat disease have been successfully employed, e.g., antisense, siRNA, aptamer, CRISPR, and mRNA, with an increasing number of drug approvals in recent years.
Alexandra Ros (Arizona State University)
HPPC and HPDEP: High Performance Protein Crystallography and Dielectrophoresis
- Dielectrophoresis (DEP) refers to the migration of polarizable particles, colloids, biomolecules, organelles, viruses, and other analytes in the presence of an electric field gradient. The large variety of analytes prone to DEP migration implies the potential to become a critical element in separation sciences adding to the toolkit for researchers seeking high performance techniques.
Guowang Xu (HPLC 2024 - China Program Chair)
Challenges and Solutions for Metabolomics and Exposomics Based on High-resolution Mass Spectrometry With/Without Chromatography
- Small molecules in the human body include the endogenous metabolites, exogenous exposure, and microbial metabolic products. The changes in these three types of compounds directly reflect human physical and mental health. By using mass spectrometry (MS) to analyze the content of exposome and the change of metabolome, the relationship between these substances and the occurrence and development of diseases can be revealed. Unfortunately, there are at least 500,000 endogenous and exogenous compounds involved in the metabolome and exposome, with varying chemical properties and significant concentration differences. Therefore, the first key to research is to achieve high coverage detection of endogenous and exogenous chemicals. Meanwhile, the identification of compounds is another crucial issue to be addressed. Moreover, to reveal the relevant mechanisms and their abundance and changes in the population, the research subjects have posed challenges to the sensitivity and repeatability of analytical instruments and methods, ranging from single cells to large-scale populations, from time to space, and from hosts to microbiota.
Gert Desmet (HPLC 2025 Program Chair)
Recent Advances in the Modeling of Chromatographic Media
- The past decades have witnessed an enormous progress in separation efficiency and peak capacity that can be achieved in 1D liquid chromatography (LC) columns in a practically affordable time. However, there are still areas where the need for more efficiency and speed is imminent. One such area is that of proteomics. Given the typical small size of proteomics samples and the need for sensitivity, micro- and nano-flow LC is the current LC method of choice in proteomics research.
Short Courses
Short courses will occur on Saturday, July 20 & Sunday, July 21.
Two-Dimensional Liquid Chromatography: Principles, Instrumentation, Method Development, and Applications
Dwight Stoll (Gustavus Adolphus College) and Mark Schure (Kroungold Analytical)
In 2DLC, sample components are fractionated by two different columns utilizing different retention mechanisms. To achieve successful 2D resolution of complex sample components, dissimilar (orthogonal) retention mechanisms are required to effectively spread the peaks throughout the available separation space.
In this course we will discuss in detail:
- Theory of 2DLC, from a practical point of view
- Instrumentation, including commercially available instruments
- Method development across a wide variety of sample types
- Applications to lipids, peptides, proteins, small and large molecule pharmaceuticals, biological extracts, industrial polymers, and surfactants
- Helpful insights that are important for achieving good results in the lab
Special topics in this course include:
- Extensive in-depth study of monoclonal antibody separation and analysis
- Using HIC columns in 2D, including desalting prior to mass spec
Students are expected to be familiar with HPLC.
Those who take this course will learn background information essential to understanding the technique and achieve practically useful results with commercial instrumentation. Many aspects of 2DLC are shared with one-dimensional HPLC such as column technologies, pumps, solvent systems, and matching the detector. However, 2DLC has some issues which are not present in one-dimensional HPLC, and these will be explained in detail so that course participants will have this knowledge prior to starting method development. We will also explore the suitable instrumentation for 2DLC, and how to process data external to the acquisition software.
Applications of comprehensive 2DLC will be shown for complex industrial and biological samples, as well as simpler applications such as column switching, target peak purity investigation, and biopolymer analysis using commercial two-dimensional chromatographic instruments.
Practical LC-MS/MS Method Development
Perry Wang (US FDA)
This course offers practical training for the practicing scientists. It will take the participants step-by-step through the concepts and techniques to develop LC-MS/MS methods. The emphasis is on practical issues associated with developing LC-MS/MS methods for small molecules. It also emphasizes problem-solving skills with examples encountered in the pharmaceutical industry and other fields.
This course will provide the participants with an updated overview and a solid working knowledge of LC-MS/MS. The participants will learn useful theoretical concepts, instrumental fundamentals and operating principles, column basics and selection guides, and key applications. After this course, the participants will be able to independently develop their own LC-MS/MS methods. New technologies and techniques, such as monolithic chromatography and hydrophilic interaction liquid chromatography (HILIC) will be presented.
Analysis of Oligonucleotide Therapeutics
Claus Rentel (Ionis Pharmaceuticals)
Oligonucleotides are a novel class of drugs with the potential to treat a wide spectrum of indications, including cancer, cardiovascular and metabolic conditions, neurological disorders, pulmonary and ophthalmic diseases. Several different modalities using oligonucleotides to treat disease have been successfully employed, e.g., antisense, siRNA, aptamer, CRISPR, and mRNA, with an increasing number of drug approvals in recent years.
The discovery, development, and manufacturing of oligonucleotide drugs necessitates the characterization and monitoring of the main drug component and its impurities by appropriate analytical techniques. The course will cover a wide variety of analytical techniques useful for this class of molecules like determination of assay, purity and the impurity profile of oligonucleotide therapeutics, confirmation of identity / sequence, examination of diastereoisomeric composition, analysis of starting materials and process intermediates, separation of positional isomers of impurities, and approaches for structural elucidation of product-related impurities.
What the instructor(s) hope to convey to the students:
Overview of analytical methods for oligonucleotide therapeutics
(u)HPLC Method Development
Michael Dong (MWD Consulting)
This 3-hour course reviews best practices, shortcuts, and tricks of the trade to help pharmaceutical and other scientists to become more successful in developing effective HPLC or UHPLC methods (on potency and ICH-compliant stability-indicating assays of pharmaceuticals) using the traditional selectivity-tuning, three-pronged method template, and universal generic gradient method approaches.
What will the student learn?
- The Traditional 5-step Approach, according to Snyder, Kirkland and Glajch
- The 3-Pronged Template Approach for Rapid Method Development
- The use of a single Universal Generic Gradient Method for pharmaceutical analysis
Field-Flow Fractionations, Size-Exclusion Chromatography, and Light Scattering Detection
Bezhan Chankvetadze (Tbilisi State University)
What will be taught?
- Short basics of stereochemistry and separation of enantiomers; 2) Comparative characteristics of gas chromatography (GC), high-performance liquid chromatography (HPLC), super/sub-critical fluid chromatography (SFC), capillary electrophoresis (CE) and capillary electrochromatography (CEC) from the viewpoint of chiral separations and enantioselective analysis; 3) Non-covalent interactions and the importance of their control for preparation and use of chiral stationary phases and chiral selectors; 4) Currently available chiral columns and chiral selectors for practical problem solving and their comparative characteristics; 5) Some unusual effects in chiral separations; 6) Understanding enantioselective recognition and chiral separations (kinetics, thermodynamics, molecular modeling).
What I hope to convey to the students?
- Understanding of critical differences between achiral and chiral separations; 2) Proper selection of technique for particular problem solving; 3) Proper selection of separation and detection conditions; 3) Understanding of major tools for adjustment and fine tuning of separation process; 4) Adequate interpretation and understanding of separation results; 5) Recent trends in chiral column development and applications.
Supercritical Fluid Chromatography
Larry Miller (Novartis)
This course will focus on fundamentals and advances in supercritical fluid chromatography (SFC) employing carbon dioxide-based mobile phases. Emphasis will be directed toward pharmaceuticals and other fields where SFC currently plays or will play a critical role such as lipidomics, specialty chemicals and polymers, biodiesel, foods and vitamins, natural products, and flavors/fragrances. Example applications in these areas will be used throughout the course as teaching illustrations.
Attendees will gain the following knowledge:
- Fundamentals of CO2-based mobile phases – why do SFC?
- Practical advantages of SFC & SFE over GC-based and HPLC-based separations and liquid-based extraction – speed, cost, selectivity, etc.
- An understanding of SFC instrumentation, how it has improved over the years, and differences between SFC and HPLC equipment.
- Method development in analytical-scale SFC and preparative-scale SFC - special considerations for scale-up
- Fundamentals of chiral SFC separations
- How to choose stationary phases for achiral and chiral SFC separations
- Which mixtures are suitable for SFC, and which mixtures may not be suitable?
- The role of modifiers/entrainers and additives in SFC and SFE separations
Capillary Electrophoresis with Mass Spectrometry Detection
David Chen (University of British Columbia)
Principles and unique characteristics of capillary electrophoresis coupled with mass spectrometry. The similarity and complementarily of CEMS with LCMS, and the unique applications that can only be performed by CEMS, will be discussed. Bioinformatic tools used for LCMS can be easily adapted for use in CEMS.
What the instructor(s) hope to convey to the students: That CEMS is a powerful analytical platform, and it is not as difficult as one might think to couple CE with MS.
HPLC Operation, Maintenance, and Troubleshooting
Martin Gilar (Waters Corporation)
The course attendees will learn about principles of chromatographic modes including reversed-phase, hydrophilic interaction, ion-exchange and size exclusion chromatography. Special emphasis will be given to reversed phase mode. Couse attendees will use MS Excel spreadsheets prepared to visualize chromatograms. Attendees will be able to change parameters such as particle size, column length, separation condition, extra column dispersion and others and instantly observe the effects in chromatographic separation.
We will cover:
- Principles of chromatographic separation, isocratic retention versus gradient
- Retention, resolution, peak capacity, band broadening, extra column dispersion
- Chromatographic behavior of small molecules versus biopolymers
- Approaches to method development and method transfer.
- SPE versus LC
- Chromatographic artifacts of large volume injection, incompatible solvents, etc.
WHO SHOULD ATTEND
Chromatography practitioners, lab personal, students, casual LC users who are interested in deeper understanding of chromatographic principles. The attendees should** bring their own laptop computers** with installed MS Excel. The attendees will be provided with excel sheets based chromatographic simulators. They will be able to perform their own in-silico chromatographic experiments.
Separation of Glycans and Glycopeptides derived from Glycoproteins
Yehia Mechref (Texas Tech University)
The separation of glycans and glycopeptides is critical for the analysis and characterization of glycoproteins, a post-translationally modified proteins with carbohydrate chains (glycans). Glycoproteins play essential roles in various biological processes, and their characterization is important in the fields of biochemistry, biotechnology, and medicine. This workshop will cover common methods for the separation of glycans and glycopeptides, including hydrophilic interaction chromatography (HILIC); reverse-phase chromatography (RPC), ion-exchange chromatography (IEC), capillary electrophoresis (CE), and affinity chromatography. The workshop will also cover hybrid methods, combining multiple separation techniques, such as liquid chromatography with mass spectrometry (LC-MS), thus enabling comprehensive analysis of glycans and glycopeptides. LC-MS is a powerful tool for the identification and quantification of glycans and glycopeptides. The choice of method depends on the specific characteristics of the glycoproteins being analyzed and the goals of the study. Researchers often use a combination of techniques to obtain a more comprehensive understanding of the glycoprotein composition.
What the instructor(s) hope to convey to the students:
Students will be introduced to the best chromatographic techniques and methods that will facilitate an effective analysis and characterization of glyans and glycopeptides derived from glycoproteins of biological, biomedical and pharmaceutical relevance.
Introduction to Capillary Liquid Chromatography
Justin Godinho (GlaxoSmithKline) and James Grinias (Rowan University, Glassboro, New Jersey)
This course is designed to introduce those familiar with analytical scale HPLC to capillary (or “nano”) liquid chromatography. Although both techniques are based on the same fundamental principles, capillary LC has a number of distinct advantages and challenges that will be detailed. Commercial instrument options, as well as the basics of preparing your own capillary LC columns, will be described. Because one of the most prominent uses of capillary LC is its coupling to mass spectrometry for complex biological sample analysis, special attention will be given to this important area. Both academic and industrial researchers will be able to apply the information gained through this course to overcome the challenges faced when using this essential technique.
After completing this course, participants will be able to:
- Understand the differences between analytical and capillary scale LC. Describe the fundamentals of capillary LC column preparation.
- Determine the best detection modes for a given application.
- Explain the advantages of coupling capillary LC with mass spectrometry and how to approach method development using capillary LC-MS.
- Identify best practices for the use of capillary LC to solve analytical challenges.
Accurate Liquid Chromatography Solutions for Cannabinoids Quantitation
Evgenia Barannikova (Shimadzu Scientific Instruments) and Nivesh K. Mittal (Shimadzu Scientific Instruments)
Cannabis ranges in availability for both medicinal use and adult use throughout the US, and different States have dedicated limits. The cannabis/hemp market continues to grow, with more States legalizing marijuana, as well as the 2018 Farm Bill removing hemp from the controlled substance list. This bill defines that any cannabis sativa L. strain with a total tetrahydrocannabinol (THC) concentration of ≤0.3% can be considered hemp, not cannabis. Consequently, there is strong demand to differentiate hemp from cannabis based on THC concentration.
This course will discuss the cannabis testing field through the lens of the most-used instruments needed for routine analysis with particular emphasis on HPLC considering that ASTM is preparing to release a standardized procedure for the determination of cannabinoids concentration using HPLC. Traditional qualitative methods use gas chromatography mass spectrometry (GCMS) to identify and confirm the presence of marijuana in criminal cases. GCMS could be used for quantitation, however full separation of the acid forms is not feasible as they are converted to native THC in the heated source. There has been a recent uptick in adulterated hemp samples containing THC variants and there is a need to fully identify and quantify each of them. This short course will describe the use of liquid chromatography (LC) techniques for accurate separation of common cannabinoids, the Shimadzu Cannabis and Hemp analyzers for THC quantitation, and mass spectrometry detection methods since LCMS has the ability to detect and quantitate regulated pesticides and mycotoxins not detectable by GCMS. In addition, LCMS can also be used for combined psychoactive component analysis when the there is a need to quantify THC isomers such as delta-8 and delta-9, delta-10 and delta-6a/10a THC. We will also discuss recommended practices surrounding sampling and sample preparation and provide an overview of our resources available for emerging labs. The troubleshooting section will serve as a guide applicable to any HPLC use, with special focus on tough matrices and routine care. To wrap up, we will discuss other equipment needed in the cannabis testing space for a fully independent testing laboratory.
Learning objectives
- Cannabis/hemp market overview.
- Learn about the cannabis or hemp analyzers for THC quantitation based on HPLC technique.
- Learn about importance of sample preparation and recommendations for sample prep.
- Learn how to expand capabilities of LC to include MS for analysis of pesticides, mycotoxins, and additional cannabinoid isomers such as delta-8 and delta-9, delta-10 and delta-6a/10a THC.
- LC troubleshooting.
- Other instruments of note to the market, such as GCMS and ICPMS.
What will be taught?
Key topics for this course will include an overview of the cannabis testing space with particular emphasis on HPLC, as the most commonly used instrument in routine testing. We will discuss our routine testing methods for THC concentration and other cannabinoids, recommended practices surrounding sampling and sample preparation, and provide an overview of our resources available for emerging labs. Due to the sample matrix complexity, sometimes things can go wrong. A large part of this course will be dedicated to troubleshooting and routine care of the HPLC to ensure best results. Other equipment may also be needed when testing safety of a given natural product; we will also briefly touch on instruments such as LCMS, GC, and ICPMS that can expand capabilities in a testing lab.
What the instructor(s) hope to convey to the students
We want attendees to complete our course with an understanding of the cannabis testing field and how important of a role HPLC plays in the safety and regulation of the product. We hope that the attendees will enjoy the troubleshooting tips and can incorporate this knowledge into their own routines. Finally, we aim to expand horizons and look around a fully outfitted lab for examples of routine tests needed to ensure consumer safety.
