Course: How to Develop HPLC Methods for Challenging Separations

Phenomenex Training Seminars 2024: How to Develop HPLC Methods for Challenging Separations

Course Summary

Learn how to implement strategies to achieve satisfactory separation for ‘complex’ samples and use computer modelling to develop robust and fit for purpose HPLC methods. This one day course is ideal for those who have experience developing HPLC methods but want to increase their knowledge to deal with more challenging separations. Although designed as a follow-up to the course “How to Develop HPLC Methods”, it is not a prerequisite.

This course is suitable for learners who feel confident that they are familiar with all the content detailed for the course “How to Develop HPLC Methods”.

Registration

Date and Location

15 October 2024

• 08:30 - 16:00 BST | 09:30 - 17:00 CEST
• Online

Price (per person)

£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Course Outline

Implementing a 5-step strategy for method development of complex samples.

Review of a 5-step strategy for HPLC method development with particular attention to reasons why some separations are ‘challenging’:

• Setting suitable objectives for method development
• Assessing all available information
• Selecting suitable samples
• Performing scouting experiments to select suitable initial conditions
• Optimising the method to define method parameters which achieve the desired separation.

Separation theory

Method development objectives: Strategies for maximising resolution in HPLC using retention factor, k’, selectivity, α, and efficiency, N.

Case Study Using Computer Modelling

A case study is used to demonstrate how a computer model may be used to find the best conditions for the separation.

Practical Skills Acquired

This course will enable you to find solutions for difficult HPLC separations. In addition you will be able to:

• Understand why some separations can be challenging.
• Identify potential problem separations.

Apply strategies to achieve satisfactory separations for “complex” samples with respect to:

• Sample preparation
• Detection methods
• Retaining very polar analytes
• Optimising gradient methods
• Selecting columns
• Setting up scouting/screening experiments