Course: An Introduction to LC-MS

Phenomenex Training Seminars 2024: An Introduction to LC-MS

Practical Skills Acquired

Attendees will learn the basics of LC-MS allowing them to select suitable columns and mobile phases for screening. They will learn about quadrupoles and time of flight mass analysers together with how to understand the data output.

Registration

Date and Location

30 April 2024

• 08:30 - 16:00 BST | 09:30 - 17:00 CEST
• Online

24 September 2024

• 08:30 - 16:00 BST | 09:30 - 17:00 CEST
• Online

Price (per person)

£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Course Outline

Part I. Intro to Liquid Chromatography

• What is the role of the LC column in LC-MS?
• Why is it even necessary?
• Basics of LC theory
• Review of the different LC media/support particles
• Survey of different LC bonded phases and their application
• Guide to selecting the right LC column for your application
• Effect of temperature
• Solvent selection
• HPLC/UHPLC column care

Part II. Introduction to LC-MS

Led by an Application Specialist from SCIEX this module will introduce and develop new practitioners by delivering an understanding of basic mechanisms associated with LC-MS/MS. As well as discussing mass spectrometry fundamentals, the material will then go on to examine the most commonly applied LC-MS techniques and the principles behind them:

Atmospheric Pressure Ionisation theory, techniques and methods:

• Desolvation
• Electrospray Ionisation (ESI)
• Atmospheric Chemical Ionisation (APCI)

Mass Analysis

• The importance of calibration
• The differences between nominal mass, accurate mass and mass resolution

Mass Analysers

• Quadrupole – benefits, limitations and general usage
• Time of Flight – benefits, limitations and general usage

Data analysis

• Quantitation
• Application of targeted techniques eg MRM to data acquisition and processing
• Qualitative approaches to identification
• Application of discovery techniques eg scanning, information dependent acquisition, library searching