Course: An Introduction to Peptide Mapping

Phenomenex Training Seminars 2024: An Introduction to Peptide Mapping

Course Summary

In this training we will discuss sample preparation and good practices that can impact the quality of your result from peptide mapping depending on your Lab’s analytical scope. The course will explore and present specific examples in Sample Preparation and Chromatography.

Registration

Date and Location

This course is split over 2 half-days. Registration cost covers both dates.

17 and 18 September 2024

• 08:30 - 12:30 BST | 09:30 - 13:30 CEST
• Online

Price (per person)

£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Course Outline

Part 1: Peptide overview

• Digestion of Proteins
• Chromatographic Selectivity
• Method Development for Peptide Analysis

Part 2: Peptide overview - cont.

• PTM Identification
• Sample preparation using Magbeads
• Analytical vs. Nano flow

Part 3: Case Studies

• Liraglutide
• Bivalirudin

Part 4: Good Laboratory Practices

Practical Skills Acquired

This course will enable you to find solutions to your Peptide Mapping challenges. In addition, you will be able to:

• Drive a chromatographic optimization for peptide mapping, including investigating differences in method parameters, designing effective gradient programs, column selecting the best particle/phase selection, among others
• Recognize the most suitable acid modifier depending on the context of the analytical method
• Choose the correct chromatography conditions and selectivity to achieve the best peak capacity and largest number of peptide/protein identifications