Course: An Introduction to LC-MS

Phenomenex Training Courses 2025: An Introduction to LC-MS

Practical Skills Acquired

Attendees will learn the basics of LC-MS allowing them to select suitable columns and mobile phases for screening. They will learn about quadrupoles and time of flight mass analysers together with how to understand the data output.

👉Registration

Date and Location

18-19 June 2025

• 08:30 - 12:30 GMT | 10:30 - 14:30 CEST
• Online

27-28 October 2025

• 08:30 - 12:30 BST | 10:30 - 14:30 CEST
• Online

Price (per person)

£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Please note: the seminars take place in two half days (2 sessions of 4 hours each).

Course Outline

Part I. Introduction to Mass Spectrometry:

This module will introduce and develop new practitioners by delivering an understanding of basic mechanisms associated with LC-MS. As well as discussing mass spectrometry fundamentals, the material will then go on to examine the most commonly applied LC-MS techniques and the principles behind them:

• Atmospheric pressure ionisation theory
  • Desolvation
  • Electrospray Ionisation (ESI)
  • Atmospheric Chemical Ionisation (APCI)
     
• Mass spectrometry definition
  • Overview of the key definitions in mass spectrometry; mass accuracy and resolution, nominal versus accurate mass and isotopic pattern identification
     
• Mass analyser: benefits, limitations and general usage
  • Quadrupole
  • Ion trap
  • Time of flight
  • Orbitrap
• Mass spectrometry workflow
  • Acquisition procedures
  • Qualitative and quantitative approach
  • Case studies

Part II. Liquid Chromatography for Mass Spectrometry:

• What is the role of the LC column in LC-MS?
• Why is it even necessary?
• Basics of LC theory
• Review of the different LC media/support particles
• Mobile phases and buffer choice for LC-MS
• Common contaminants with MS