Course: HPLC Method Development

Phenomenex Training Courses 2025: HPLC Method Development

Course Summary

Learn how to select appropriate method conditions to obtain a set of method parameters which enables the desired separation for mixtures of analytes. This course is ideal for those who have experience running HPLC methods and now want to learn how to develop new methods.

👉Registration

Date and Location

22-23 May 2025

• 08:30 - 12:30 GMT | 10:30 - 14:30 CEST
• Online

15-16 October 2025

• 12:00 - 16:00 GMT | 14:00 - 18:00 CEST
• Online

Price (per person)

£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)

Please note: the seminar takes place in two half days (2 sessions of 4 hours each).

Course Outline

From Analyte to Column Choice:

• Analyte chemical properties
• Column dimension
• Stationary phase selectivities and interaction
• Special phases (High pH stable column, Polymeric and Polar-modified column)

From Mobile Phase to Gradient Slope:

• Selectivities of organics
• Gradient slope
• Flow rate, temperature, connections, injection program and sample diluent
• Walking through case examples

Practical Skills Acquired

This course will enable you to take a strategic approach to method development with an understanding of the key chromatographic factors. In addition you will be able to:

• Link the chemical properties of the analytes to the key chromatographic parameters to successfully develop an HPLC analytical method.
• Select the best column dimension and stationary phase to fully resolve your mixture.
• Select suitable scouting conditions to find a suitable column and mobile phase system.
• Select and prepare a suitable sample or samples to be used for the method development.
• Optimise the chromatographic conditions to result in the best possible separation.