Course: Troubleshoot HPLC - Identifying, Solving and Avoiding Problems
Phenomenex Training Courses 2025: Troubleshoot HPLC: Identifying, Solving and Avoiding Problems
Course Summary
Learn how to find solutions to problems encountered when running HPLC analysis by diagnosing symptoms and implementing appropriate preventative measures. This course is ideal for those who have experience using HPLC and now want to develop their skills further.
Date and Location
1-2 July 2025
- 12:00 - 16:00 GMT | 14:00 - 18:00 CEST
- Online
18-19 November 2025
- 08:30 - 12:30 GMT | 09:30 - 13:30 CET
- Online
Price (per person)
£425 | 485 € | 480 CHF | 1200 PLN | 3600 DKK | 5100 NOK | 5350 SEK | 9300 CZK (+VAT, incl. PDF of the slides and certificate of attendance)
Please note: the seminar takes place in two half days (2 sessions of 4 hours each).
Course Outline
Problem Solving Strategy:
- Leaks
- Variable retention times
- Quantitation and data quality
- Baseline
- System effects
General Chromatographic Problems:
- Assessing the symptoms
- Making the diagnosis
- Finding the appropriate solution
- System problem, preventive maintenance and column care
Peak Problems:
- Fronting and tailing
- Peak split
- Negative and ghost peaks
- Real case study
Practical Skills Acquired
This course will enable you to go back to your lab with a full understanding of why problems may arise with your HPLC system and give you the skills and knowledge to both prevent and resolve those problems. In addition you will be able to:
- Approach and follow the general steps on HPLC troubleshooting.
- Troubleshoot general chromatographic problems, including pressure, leaks, variable retention times, quantitation, data quality and baseline issues.
- Identify the most common peak issues and the possible causes.
- Clean/regenerate the HPLC column.
