Advanced analytics for proteins and biological nanoparticles

Join us for a day dedicated to the characterization of proteins and complex protein conjugates. Dive into the multi-attribute quantification of mRNA-lipid nanoparticle therapeutics, exploring aspects such as size, particle concentration, and size-based nucleic acid payload distribution. Engage in application-driven discussions and refresh your knowledge on the characterization of LNPs, AAVs, and lentiviruses.

Powerful analytical techniques will be showcased. Questions of identity, purity, quantity, and payload content will be addressed using advanced methods from Waters, Wyatt, and TA Instruments, ensuring the highest quality of your samples.

Learn about the capabilities of light scattering, charge detection mass spectrometry (CDMS), differential scanning calorimetry (DSC), and advanced process analytical tools, and see how these methods can complement your existing suite of analytical tools.

During the coffee and lunch breaks, don't miss the chance to see Andrew+, the pipetting robot, in action. Experience firsthand how the Andrew+ sample preparation robot can streamline your workflow, enhancing reproducibility and efficiency. Automate pipetting and sample prep tasks and ensure precise, high-quality sample preparation for your experiments.

Topics covered:

• Ensuring sample quality of proteins and biological nanoparticles
• Enhanced analysis of macromolecules and nanoparticles through DLS/SLS/ELS
• Characterization of protein conjugates using SEC-MALS
• MaxPeak HPS technology that prevents non-specific binding for your analytics in SEC chromatography
• Q&A and individual consultations with Technology scientists

Who should attend:

• Scientists who are interested in screening as well as in-depth characterization of proteins, protein conjugates, LNPs, AAVs, lentiviruses, and other viral vectors.
• People requiring sample quality assessment for formulation studies.
• Researchers in the field of gene therapy.
• Researchers looking for Process analytical tools to monitor and control downstream processes for many modalities including viral vectors, nanoparticles, recombinant proteins, nucleic acids and biopolymers.
• Active users of Wyatt, Waters or TA instruments seeking to explore other analytes in the Waters portfolio for biologics and would like to engage with regional colleagues around similar analytical challenges and wish to learn more about advanced topics related to Waters instruments and software.
• Researchers who are enthusiastic about automated sample preparation workflows and want to explore complementary analytical techniques for improved data quality and efficiency.

Registration

Registration is free for this event, seats are limited. Coffee and lunch are provided.

Register here

Program

08:30 Registration, Tea and Coffee

09:00 Welcome address

09:40 Why sample quality matters

Applying Light Scattering tools to ensure sample quality, an introduction to Light Scattering fundamentals.

10:10 Exploring Enhanced Particle Analysis through the Integration of DLS, ELS, & SLS

Automated Light-Scattering solutions for fast screening and characterization of size, stability, charge, and zeta-potential.

10:30 Coffee break

11:00 AAV characterization using SEC-MALS and AEX

• MSc. ir. S. Blokland (Johnson & Johnson Innovative Medicine)

11:30 Towards Well-Characterized Proteins and Protein Conjugates Using SEC-MALS

Uncover the analytical power of SEC-MALS in characterizing oligomers and different protein conjugates, such as ADCs, AOCs, and RNPs.

12:00 Lunch break

13:00 Light Scattering Solutions for Multi-Attribute Quantification of Adeno-Associated Viruses (AAVs) and Lipid Nanoparticles (LNPs)

Measuring critical quality attributes (CQAs) of AAVs and LNPs with an integrated light-scattering solution for size, payload, and concentration.

13:45 Ultra-Sensitive DSC Technologies for in-depth Thermodynamic Investigations and High Throughput Screening of Protein Stability

• Dr. Peter Vikegard (TA Instruments)

14:15 Short Prokaryotic Argonaute Systems trigger Cell Death upon Detection of Invading DNA

• Dr. Simon Lindhoud (Wageningen University & Research)

15:00 Coffee Break

15:30 Solutions for In-Process Monitoring of Critical Quality Attributes in Bioprocessing

16:00 The Potential of Light Scattering in Process Analytical Technology for Fast and Reliable Analyses

16:30 END

Location

• Hilton Garden Inn Leiden, Willem Einthovenstraat 3, Oegstgeest, 2342 BH, Netherlands