<p><strong>C18 phases do not provide sufficient retention for polar compounds. The result is often coelution and signal suppression in LC–MS. When analyzing polar analytes, C18 is therefore usually not the appropriate choice.</strong></p><p>HILIC (Hydrophilic Interaction Liquid Chromatography) represents a different separation approach.</p><h4><strong>Principle of HILIC</strong></h4><p>The initial mobile phase composition is<strong> 90% acetonitrile </strong>and 1<strong>0% ammonium buffer</strong>. The high organic content creates a thin water-enriched layer on the polar stationary phase. Analytes partition into this layer, and as the water content in the mobile phase increases, they elute.</p><h3><strong>Method Development Essentials</strong></h3><h4><strong>1. Selection of the Stationary Phase</strong></h4><p>Choose an appropriate polar stationary phase, for example:</p><ul><li>silica</li><li>diol</li><li>amino</li><li>zwitterionic</li></ul><h4><strong>2. Initial Mobile Phase Conditions</strong></h4><ul><li>Start at 90% acetonitrile with an ammonium buffer.</li><li>Do not use methanol — it disrupts the water-enriched layer and affects retention.</li></ul><h4><strong>3. Gradient Strategy</strong></h4><p>In HILIC, <strong>water is the strong eluent</strong>. The gradient therefore starts at high organic content and then progressively increases the water content.</p><h4><strong>4. pH Optimization</strong></h4><p><strong>Compare:</strong></p><ul><li>pH 3.2 (ammonium formate)</li><li>pH 5.8 (ammonium acetate)</li></ul><p>Check the differences.</p><h4><strong>5. Equilibration</strong></h4><p>Ensure proper equilibration of the system.</p><h3><strong>Practical Note</strong></h3><p>Excess water in the sample disrupts HILIC retention and leads to poor peak shape.</p>

Stop Using C18 for Polar Analytes

<h4><strong>A New Standard in HPLC Column Connection by SIELC</strong></h4><p><strong>SIELC Technologies, a pioneer in chromatography innovation, introduces a single-ended HPLC column — a revolutionary new design that redefines how columns are connected and used in high-performance liquid chromatography. For decades, laboratories have relied on the same standard column format — and have dealt with the same recurring problems: leaky fittings, clogged junctions, dead volume, and the constant hassle of tubing and connection selection.</strong></p><p><strong>SIELC has developed a single-ended HPLC column design.</strong></p><p>This new system redefines how columns are installed, used, and replaced — streamlining your workflow and eliminating common points of failure.</p><h5><strong>Key Benefits of the New Design:</strong></h5><ul><li><strong>No fittings or tubing required</strong> — eliminates leaks, complex installation, and the need to match tubing length or ID.</li><li><strong>Zero dead volume</strong> thanks to a direct connection via a dedicated adapter.</li><li><strong>Leak containment</strong> — in case of pressure failure, solvent remains inside the adapter with no external leakage.</li><li><strong>Tool-free column replacement</strong> — disconnect the column without touching the fittings.</li><li><strong>Aluminum body</strong> — ensures efficient heat transfer and provides a smooth, label-friendly surface even on the shortest columns.</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_HPLC_column_3af7eb512a.jpg" alt="SIELC: HPLC column" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_HPLC_column_3af7eb512a.jpg 171w," sizes="100vw" width="1600" height="1459"></p><h4><strong>HPLC Column Adapter</strong></h4><p>The SIELC adapter is a key component of the new single-ended column system, designed to eliminate leaks, dead volume, and connection complexity in HPLC setups.</p><p>This adapter is an engineering solution that ensures perfect sealing, precise flow alignment, and reliable performance under high pressure — all without traditional fittings, ferrules, or tools.</p><ul><li><strong>Dimensions:</strong> 21 × 21 × 26 mm (0.83 × 0.83 × 1.02 in)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_HPLC_Column_Adapter_8495244726.jpg" alt="SIELC: HPLC Column Adapter" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_HPLC_Column_Adapter_8495244726.jpg 165w," sizes="100vw" width="918" height="868"></p><blockquote><p><strong>Special offer:</strong> First-time buyers receive a Free Single-Column Adapter with their purchase. Experience the new connection standard — no fittings, no leaks, no extra cost.</p></blockquote><h4><strong>How the Single-Ended HPLC Column Works?</strong></h4><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_How_the_Single_Ended_HPLC_Column_Works_24ebf8b072.jpg" alt="SIELC: How the Single-Ended HPLC Column Works?" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_How_the_Single_Ended_HPLC_Column_Works_24ebf8b072.jpg 245w," sizes="100vw" width="1600" height="474"></p><p>As the mobile phase enters through the <strong>MP Inlet</strong>, it flows into the adapter’s internal capillary aligned with the column axis. When the mobile phase passes through the column packing bed, it exits through the bypass channel connected with the <strong>MP Outlet</strong>.</p><p>This design eliminates dead volume, prevents mobile-phase leakage, and provides a simple way to remove and replace the column when needed.</p><h3><strong>Dual-Ended vs. Single-Ended HPLC Columns</strong></h3><h4><strong>Dual-Ended HPLC Columns</strong></h4><ul><li><strong>Column connections: </strong>Dual-ended HPLC columns have two separate ends. Each connection requires fittings, ferrules, and tubing to connect the column to the LC system.</li><li><strong>Installation: </strong>Installation requires manual alignment of fittings and precise adjustment of tubing length to minimize dead volume. This process can be time-consuming and is prone to user error.</li><li><strong>Dead volume: </strong>Dead volume is typically present at both the inlet and outlet connections. These junctions can cause peak broadening and reduced chromatographic efficiency.</li><li><strong>Leakage risk: </strong>Leakage risk is relatively high because solvent connections rely on ferrules and fittings that may loosen or wear over time.</li><li><strong>System integration: </strong>Dual-ended columns require separate tubing and careful fitting when installed in column heaters or coolers.</li><li><strong>Thermal performance: </strong>Heat transfer may be less uniform due to multiple connections and tubing interfaces.</li><li><strong>Guard column option: </strong>Using a guard column requires additional hardware and extra connections, which can introduce additional dead volume into the system.</li><li><strong>Available phases: </strong>Dual-ended columns are typically available in standard stationary phases such as C18, HILIC, ion exchange, and others.</li></ul><h4><strong>Single-Ended HPLC Columns</strong></h4><ul><li><strong>Column connections: </strong>Single-ended columns use a single coaxial connection with an integrated adapter. No fittings, ferrules, or tubing are required.</li><li><strong>Installation: </strong>The adapter is installed only once. After that, the column can be replaced in seconds without tools, making maintenance faster and simpler.</li><li><strong>Dead volume: </strong>Near-zero dead volume is achieved because the internal capillaries are precisely aligned within the adapter.</li><li><strong>Leakage risk: </strong>The mobile phase is fully contained inside the adapter, creating a leak-free design even under high pressure conditions.</li><li><strong>System integration: </strong>A compact stainless-steel adapter allows easy installation in column heaters or coolers.</li><li><strong>Thermal performance: </strong>The aluminum outer body provides efficient and uniform heat transfer across the column.</li><li><strong>Guard column option: </strong>An integrated zero-dead-volume guard column option is available, allowing quick cartridge replacement without tools.</li><li><strong>Available phases: </strong>All SIELC phases as well as traditional chromatographic phases (C18, HILIC, ion exchange, mixed-mode, BIST™, etc.) are available in this format.</li></ul><h4><strong>Adapter for Guard Column</strong></h4><p>If your workflow requires a guard column or a filter to be installed inline with the main column, then a specialized adapter with a guard option should be used.</p><p>The SIELC HPLC Single-Column Guard protects the analytical column by capturing particulates and strongly retained contaminants before they reach the stationary phase. Its unique direct-insert design provides a zero-dead-volume connection and allows quick, tool-free replacement of a used guard with a new one. Guards are available for all SIELC stationary phase types — <strong>Primesep™, Newcrom™, Chromni™, PEI™, BIST™</strong>, and for generic phases such as C18, HILIC and IE.</p><h5><strong>Guard Cartridges (2 per pack)</strong></h5><ul><li><strong>Guard Adapter — Dimensions: </strong>21 × 21 × 42 mm (0.83 × 0.83 × 1.65 in)</li></ul><p><img src="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/SIELC_guard_cartridges_4077d1edf2.jpg" alt="SIELC: Guard cartridges" srcset="https://storage.googleapis.com/labrulez-bucket-strapi-h3hsga3/thumbnail_SIELC_guard_cartridges_4077d1edf2.jpg 245w," sizes="100vw" width="768" height="262"></p><h4><strong>Why This Matters</strong></h4><p>This new column system removes the guesswork and fragility from high-pressure liquid chromatography. By eliminating traditional high-pressure fittings and minimizing dead volume, you gain more consistent results, lower the risk of system failure, and reduce downtime.</p><p>Whether you’re running routine analyses or complex separations, this design enhances reliability and usability — so you can focus on your science, not your setup.</p><blockquote><h5><strong>All SIELC and Traditional Columns and Guards Available in a New Format</strong></h5><p>C18, HILIC, Ion Exchange, Mixed-Mode, Ion Exclusion, Protein, BIST, and more.</p></blockquote><blockquote><p>📄<strong> Want to learn more?</strong> <a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/labrulez-bucket-strapi-h3hsga3/Single_ended_column_new_2_1_b80be5f107.pdf"><strong>Download our brochure</strong></a> to learn about a single-ended HPLC column design.</p></blockquote>

Single-Ended HPLC Column by SIELC

<p><strong>Symposium "Advances in Chromatography and Electrophoresis &amp; Chiranal" belongs to the periodical meetings of specialists working in the field of separation methods. The biennial conference is organized by Department of Analytical Chemistry, Faculty of Science, Palacky University in cooperation with Czech Chemical Society and Czech Society for Mass Spectrometry. It is organized as a meeting of the scientists from the universities, academic institutes and experts from the routine analytical laboratories.</strong></p><p><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/chiranal/programme/"><strong>The scientific program</strong></a> will deal with all areas of separation science from fundamental to applied reseach in chemistry, physics, biology, medicine, forensic science and related disciplines. Official languages of the conference are English, Czech and Slovak.</p><ul><li><strong>Date: </strong>15. - 18. June 2026</li><li><strong>Place:</strong> Olomouc, Czech Republic</li><li><strong>Contact: </strong>prof. RNDr. Petr Bednář, Ph.D. (Department of Analytical Chemistry, Faculty of Science, Palacky University)<ul><li><strong>e-mail: </strong>petr.bednar@upol.cz</li></ul></li></ul><h3><strong>Registration</strong></h3><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/chiranal/registration/"><strong>Register for the conference HERE</strong></a></p></blockquote><ul><li><strong>Conference Fees:</strong> 6.000,- CZK / 250 EUR &nbsp;without VAT</li><li><strong>Deadline for registration is May 31st, 2026.</strong></li><li>You will receive a registration confirmation by email.</li></ul><h3><strong>Abstract Submission</strong></h3><blockquote><p class="cke-user_style-important"><strong>👉</strong><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/chiranal/abstract-submission/"><strong>Submit your Abstract HERE</strong></a></p></blockquote><ul><li><a target="_blank" rel="noopener noreferrer" href="https://www.upol.cz/fileadmin/users/381/template.docx"><strong>Download Abstract Template</strong></a></li><li>The <strong>deadline for submitting</strong> abstracts for <strong>oral presentations</strong> and posters is <strong>May 11, 2026</strong>.</li></ul><h3><strong>Preliminary Programme</strong></h3><h4><strong>Monday 15th June 2026, Fort Science</strong></h4><h5><strong>16:15 - 17:15 Plenary Lecture</strong></h5><ul><li>Michael Laemmerhofer</li></ul><h4><strong>Tuesday 16th June 2026</strong></h4><h5><strong>11:30 - 12:00 Enantioselective Ion Pairing Systems, my 50 years of Contribution</strong></h5><ul><li>Wolfgang Lindner&nbsp;</li></ul><h5><strong>16:30 - 17:00 Data science-assisted method development for on-line supercritical fluid extraction – chromatography</strong></h5><ul><li>Kevin Schug (<a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/301"><strong>The University of Texas at Arlington</strong></a>)</li></ul><h4><strong>Wednesday 17th June 2026</strong></h4><h5><strong>9:00 – 9:30 The Role of Analytical Chemistry in Solving Contemporary Challenges in Biomethane Plants</strong></h5><ul><li>Justyna Płotka-Wasylka</li></ul><h5><strong>11:30 - 12:00 Rational approaches to MALDI-MS matrix design</strong></h5><ul><li>Hans-Dieter Junker</li></ul><h5><strong>12:50 - 13:10 Using Charge Detection Mass Spectrometry with an Electrostatic Linear Ion Trap for the Analysis of Viral Vectors and Large Protein Complexes</strong></h5><ul><li>Alex Muck (Waters)</li></ul><h4><strong>Thursday 18th June 2026</strong></h4><h5><strong>9:00 – 9:30 Capillary Electrophoresis - Mass Spectrometry for the characterization of proteoforms on the intact level</strong></h5><ul><li>Christian Neusüß</li></ul><h5><strong>9:30 – 10:00 Investigation of acid-base and electromigration properties of biopeptides by capillary electrophoresis</strong></h5><ul><li>Václav Kašička</li></ul><h5><strong>11:30 - 12:00 Open-source capillary electrophoresis - democratizing separation science: Design principles and practical implementations from our laboratory</strong></h5><ul><li>Petr Kubáň</li></ul><h5><strong>12:00 - 12:30 Analysis of biomedical, environmental and pharmaceutical samples by microchip electrophoresis</strong></h5><ul><li>Marián Masár</li></ul><h5><strong>12:30 - 13:00 UV-LED coupling for high-sensitivity glycan analysis in commercial capillary electrophoresis system</strong></h5><ul><li>Jana Lavická</li></ul><h4><strong>Sponsors</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/companies/37"><strong>Altium International s.r.o. (HPST)</strong></a></li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.cz/companies/50"><strong>Waters Gesellschaft m.b.H.</strong></a></li><li>Teva</li></ul><h4><strong>Partners</strong></h4><ul><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/341"><strong>Department of Analytical chemistry, Palacký University Olomouc</strong></a></li><li>Faculty of Science, Palacký University Olomouc</li><li>SMALT (Spojené laboratoře pro materiálové a analytické technologie)</li><li>Czech society for mass spectrometry</li><li>Česká společnost chemická</li><li>ARTECA</li><li>Pevnost poznání</li><li>Odborná skupina chromatografie a elektroforézy ČSCH</li><li><a target="_blank" rel="noopener noreferrer" href="https://lcms.labrulez.com/companies/1"><strong>LabRulez</strong></a></li></ul><h4><strong>Conference site</strong></h4><h5><strong>Address of conference site:</strong></h5><ul><li>Faculty of Science</li><li>Palacky University</li><li>17. listopadu 1192/12</li><li>779 00 Olomouc</li></ul>

Advances in Chromatography and Electrophoresis & Chiranal 2026 - Registration and Abstract submission open!

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Symposium belongs to the periodical meetings of specialists working in the field of separation methods.

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Advances in Chromatography and Electrophoresis & Chiranal 2026 - Registration and Abstract submission open!

Single-Ended HPLC Column by SIELC

Stop Using C18 for Polar Analytes