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SEC Coupled to High-Resolution Mass Spectrometry for Detailed Characterization of mAbs and ADCs

Applications | 2018 | Agilent TechnologiesInstrumentation
LC/TOF, LC/HRMS, LC/MS, LC/MS/MS, GPC/SEC
Industries
Pharma & Biopharma
Manufacturer
Agilent Technologies
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Application Note Biologics Efficient Monoclonal Antibody and Antibody Drug Conjugate Desalting Prior to Mass Spectrometry Using AdvanceBio Desalting-RP Cartridges Authors Isabel Vandenheede, Pat Sandra, and Koen Sandra Research Institute for Chromatography (RIC) President Kennedypark 26 B-8500 Kortrijk Anne Blackwell and…
Key words
herceptin, herceptincounts, countsdesalting, desaltingpapain, papaindeconvoluted, deconvolutedamu, amuidez, idezadvancebio, advancebiomass, masslinker, linkercartridge, cartridgedtt, dttdigested, digestedfab, fabmabs
Agilent-NISTmAb Intact & Subunit Analysis Using Reversed Phase Agilent BioHPLC Columns Application Compendium Contents Agilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted from NISTmAb RM 8671 batch. Quality control (QC) testing is performed using Agilent LC-MS system. QC batch release test…
Key words
counts, countsmab, mabherceptin, herceptinintact, intactpage, pagecontents, contentsbonds, bondsback, backdeglycosylated, deglycosylatedmass, massdeconvoluted, deconvolutedides, idesamu, amubravo, bravoassaymap
Characterize Fab and Fc Fragments by Cation-Exchange Chromatography Application Note Biologics and Biosimilars Authors Abstract Isabel Vandenheede, Emmie Dumont, This application note describes the use of the Agilent Bio MAb weak Pat Sandra, and Koen Sandra cation-exchange column in measuring…
Key words
fab, fabpapain, papainfragment, fragmentcex, cexmab, mabdeconvoluted, deconvolutedcounts, countsdeamidation, deamidationamu, amuvariants, variantscation, cationweak, weakexchange, exchangenonstressed, nonstressedbio
Application Note Pharma & Biopharma Sensitive Native Mass Spectrometry of Macromolecules Using Standard Flow LC/MS Author David L. Wong Agilent Technologies, Inc. Abstract Native mass spectrometry can be used for a variety of protein-based applications, such as protein-protein interaction, protein-ligand…
Key words
native, nativeprotein, proteincounts, countsintact, intactdeconvoluted, deconvolutedamu, amumyoglobin, myoglobinmass, massapomyoglobin, apomyoglobincharge, chargecomplexes, complexesheme, hemeholomyoglobin, holomyoglobintetramer, tetramerdenatured
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