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Application NoteCell Analysis andMetabolomicsExtracellular Flux Analysis and 13CStable-Isotope Tracing RevealsMetabolic Changes in LPS-StimulatedMacrophagesAuthorsAgnieszka Broda and GeraldLarrouy-MaumusMRC Centre for MolecularBacteriology and Infection,Department of Life Sciences,Faculty of Natural Sciences,Imperial College London,London, SW7 2AZ, UKSufyan PandorAgilent Technologies, Inc.Cheadle, UKAbstractEukaryotic cells are known to...
Key words
lps, lpsseahorse, seahorsemetabolic, metabolicmacrophages, macrophagescells, cellsphenotype, phenotypemitochondrial, mitochondrialmetabolite, metaboliteecar, ecarglycolytic, glycolyticmacrophage, macrophagetracing, tracinginflammatory, inflammatorycell, cellmetabolites
Application NoteMetabolomicsC Glucose Qualitative Flux Analysisin HepG2 cells13Using an Agilent 6546 LC/Q-TOF and VistaFluxAuthorsSiriluck Wattanavanitchakornand Sarawut JitrapakdeeDepartment of Biochemistry,Faculty of Science,Mahidol University,Bangkok, ThailandIsrar Ansari,Melissa J. Longacre, andMichael J. MacDonaldChildren’s Diabetes Center,University of WisconsinSchool of Medicine and PublicHealth, Madison, WI, USAMahmoud...
Key words
knockdown, knockdownisotopologues, isotopologuesflux, fluxcarboxylase, carboxylaseisotope, isotopeglucose, glucoseprofinder, profindertca, tcaglutamate, glutamatepyruvate, pyruvateomix, omixanaplerotic, anapleroticmetabolite, metaboliteisotopologue, isotopologueabundance
AUTOMATED HIGH-THROUGHPUT FLUX ANALYSIS OF NON-SMALL CELL LUNG CARCINOMA CELLS GROWN INVITRO IN TWO AND THREE DIMENSIONSDavid Heywood1, Agnes Corbin2, Abhishek Jha3, Raghav Sehgal3, Johannes PC Vissers1 and Amrita Cheema41 Waters Corporation, Wilmslow, UK, 2 Nonlinear Dynamics, Newcastle upon Tyne,...
Key words
monolayer, monolayercell, cellvitro, vitrospheroids, spheroidsspheroid, spheroidglucose, glucosecultures, culturesgrown, grownmonolayers, monolayerstca, tcafindings, findingstranslating, translatingglycolysis, glycolysispipeline, pipelinewere
[ TECHNOLOGY BRIEF ]Automating Metabolic Flux Analysis with Symphony and PollyDavid Heywood and Hans Vissers, Waters Corporation, Wilmslow, UK; Abhishek Jha, Elucidata, Cambridge, MA, USA;Raghav Sehgal, Kailash Yadav, and Sahil Kumar, Elucidata, New Delhi, IndiaIncrease throughput and reproducibility in metabolomicsand...
Key words
symphony, symphonydata, dataflux, fluxpipeline, pipelineelmaven, elmavenmetabolic, metabolicprocessing, processingincorporation, incorporationtools, toolscustomized, customizedbrief, briefiterative, iterativedesigned, designedstudies, studiesvisualize
 

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Application NoteCell Analysis andMetabolomicsExtracellular Flux Analysis and 13CStable-Isotope Tracing RevealsMetabolic Changes in LPS-StimulatedMacrophagesAuthorsAgnieszka Broda and GeraldLarrouy-MaumusMRC Centre for MolecularBacteriology and Infection,Department of Life Sciences,Faculty of Natural Sciences,Imperial College London,London, SW7 2AZ, UKSufyan PandorAgilent Technologies, Inc.Cheadle, UKAbstractEukaryotic cells are known to...
Key words
lps, lpsseahorse, seahorsemetabolic, metabolicmacrophages, macrophagescells, cellsphenotype, phenotypemitochondrial, mitochondrialmetabolite, metaboliteecar, ecarglycolytic, glycolyticmacrophage, macrophagetracing, tracinginflammatory, inflammatorycell, cellmetabolites
Application NoteMetabolomicsC Glucose Qualitative Flux Analysisin HepG2 cells13Using an Agilent 6546 LC/Q-TOF and VistaFluxAuthorsSiriluck Wattanavanitchakornand Sarawut JitrapakdeeDepartment of Biochemistry,Faculty of Science,Mahidol University,Bangkok, ThailandIsrar Ansari,Melissa J. Longacre, andMichael J. MacDonaldChildren’s Diabetes Center,University of WisconsinSchool of Medicine and PublicHealth, Madison, WI, USAMahmoud...
Key words
knockdown, knockdownisotopologues, isotopologuesflux, fluxcarboxylase, carboxylaseisotope, isotopeglucose, glucoseprofinder, profindertca, tcaglutamate, glutamatepyruvate, pyruvateomix, omixanaplerotic, anapleroticmetabolite, metaboliteisotopologue, isotopologueabundance
AUTOMATED HIGH-THROUGHPUT FLUX ANALYSIS OF NON-SMALL CELL LUNG CARCINOMA CELLS GROWN INVITRO IN TWO AND THREE DIMENSIONSDavid Heywood1, Agnes Corbin2, Abhishek Jha3, Raghav Sehgal3, Johannes PC Vissers1 and Amrita Cheema41 Waters Corporation, Wilmslow, UK, 2 Nonlinear Dynamics, Newcastle upon Tyne,...
Key words
monolayer, monolayercell, cellvitro, vitrospheroids, spheroidsspheroid, spheroidglucose, glucosecultures, culturesgrown, grownmonolayers, monolayerstca, tcafindings, findingstranslating, translatingglycolysis, glycolysispipeline, pipelinewere
[ TECHNOLOGY BRIEF ]Automating Metabolic Flux Analysis with Symphony and PollyDavid Heywood and Hans Vissers, Waters Corporation, Wilmslow, UK; Abhishek Jha, Elucidata, Cambridge, MA, USA;Raghav Sehgal, Kailash Yadav, and Sahil Kumar, Elucidata, New Delhi, IndiaIncrease throughput and reproducibility in metabolomicsand...
Key words
symphony, symphonydata, dataflux, fluxpipeline, pipelineelmaven, elmavenmetabolic, metabolicprocessing, processingincorporation, incorporationtools, toolscustomized, customizedbrief, briefiterative, iterativedesigned, designedstudies, studiesvisualize
 

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