Workflow Automation for LC/MS: In-Solution Protein Digestion, Peptide Cleanup, and Strong Cation-Exchange Fractionation of Peptides Enabled by AssayMAP Technology
Applications | 2016 | Agilent TechnologiesInstrumentation
Sample Preparation, LC/TOF, LC/HRMS, LC/MS, LC/MS/MS
IndustriesClinical Research
ManufacturerAgilent Technologies
Key wordsassaymap, fractionation, wise, peptides, peptide, scx, eic, automation, cartridges, through, elution, wash, increasing, multistep, bravo, assay, plates, were, developers, ionic, strength, sample, buffer, step, protocol, protocols, using, fractions, fraction, workflows, processed, flow, six, workflow, buffers, platform, exchange, cation, digestion, from, solution, hvailgdlqgpk, same, distinct, launched, cleanup, deck, priming, demonstrating, fractionated
Similar PDF
Automation for LC/MS Sample Preparation: High Throughput In-Solution Digestion and Peptide Cleanup Enabled by the Agilent AssayMAP Bravo Platform
2016|Agilent Technologies|Applications
Automation for LC/MS Sample Preparation: High Throughput In-Solution Digestion and Peptide Cleanup Enabled by the Agilent AssayMAP Bravo Platform Application Note Clinical Research Authors Abstract Jason D. Russell, Protein and peptide sample preparation for LC/MS remains entrenched in Zachary Van…
Key words
peptide, peptidecleanup, cleanupassaymap, assaymapdigestion, digestionpeptides, peptidesprotocol, protocoldenaturation, denaturationbravo, bravobsa, bsadigest, digestguanidine, guanidineplates, platesplate, platewere, weresample
Agilent AssayMAP Bravo Technology Enables Reproducible Automated Phosphopeptide Enrichment from Complex Mixtures Using High‑Capacity Fe(III)‑NTA Cartridges
2016|Agilent Technologies|Applications
Agilent AssayMAP Bravo Technology Enables Reproducible Automated Phosphopeptide Enrichment from Complex Mixtures Using High‑Capacity Fe(III)‑NTA Cartridges Application Note Automated Peptide Sample Preparation for LC/MS Authors Abstract Jason D. Russell and Steve Murphy Immobilized metal affinity chromatography (IMAC) using a nitrilotriacetic…
Key words
phosphopeptide, phosphopeptidephosphopeptides, phosphopeptidesenrichment, enrichmentassaymap, assaymapnta, ntaiii, iiiselectivity, selectivityidentifications, identificationscartridges, cartridgescasein, caseinimmobilized, immobilizeddistinct, distinctsample, samplecartridge, cartridgeimac
Automation of Sample Preparation for Accurate and Scalable Quantification and Characterization of Biotherapeutic Proteins Using the Agilent AssayMAP Bravo Platform
2016|Agilent Technologies|Applications
Automation of Sample Preparation for Accurate and Scalable Quantification and Characterization of Biotherapeutic Proteins Using the Agilent AssayMAP Bravo Platform Application Note Automated Protein Sample Preparation for LC/MS Authors Abstract Michael Bovee, Jason Russell, The Agilent AssayMAP Bravo platform automates…
Key words
assaymap, assaymapbravo, bravocartridges, cartridgesaffinity, affinityantibody, antibodypurification, purificationcoverage, coverageeluted, elutedchymotrypsin, chymotrypsinplatform, platformpeptide, peptideprotein, proteinworkflow, workflowworkflows, workflowspeptides
Rapid Antibody Digestion Enabled by Automated Reversed-Phase Desalting on the Agilent AssayMAP Bravo Platform
2016|Agilent Technologies|Applications
Rapid Antibody Digestion Enabled by Automated Reversed-Phase Desalting on the Agilent AssayMAP Bravo Platform Application Note Authors Introduction Michael Bovee, Adam Krahenbuhl, and Monoclonal antibodies are a rapidly growing class of biotherapeutics whose rigorous characterization by mass spectrometry requires reproducible…
Key words
assaymap, assaymapcartridges, cartridgesdigestion, digestionantibodies, antibodiesbravo, bravoload, loadwere, werewqqg, wqqgprotein, proteinrecovery, recoverycleanup, cleanupantibody, antibodyeluent, eluentdesalted, desaltedeluate