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Application NotePharma & BiopharmaHPLC-DAD Analysis of NucleotidesUsing a Fully Inert FlowpathAgilent 1260 Infinity II Bio-Inert LC System and aPEEK-lined Agilent InfinityLab Poroshell 120 HILIC-ZColumnAuthorsKoen Sandra,Jonathan Vandenbussche,Gerd Vanhoenacker,Ruben t’Kindt, and Pat SandraResearch Institute forChromatography (RIC),President Kennedypark 26,Kortrijk, BelgiumSonja Krieger, Sonja Schneider,and...
Key words
lined, linedpeek, peekmau, mauatp, atpstainless, stainlessinert, inertadp, adpamp, ampsteel, steelresponse, responsenucleotides, nucleotidescolumn, columnctp, ctpump, umputp
Application NoteBiopharmaEvaluation of Different Ion-PairingReagents for LC/UV and LC/MSAnalysis of OligonucleotidesAuthorsGerd Vanhoenacker,Cindy Lecluyse, Griet Debyser,Pat Sandra, and Koen SandraRIC BiologicsBelgiumSonja Schipperges,Sonja Schneider, andUdo HuberAgilent Technologies, Inc.GermanyAbstractThe effect of various ion pairing reagents (amines and acidic counterions) on theLC/UV and LC/MS...
Key words
hfip, hfipmobile, mobilecounts, countsrna, rnateaa, teaaphase, phasephases, phasesons, onsalternative, alternativedna, dnaamine, aminetea, tealadder, ladderdeconvoluted, deconvoluteddad
[ APPLICATION NOTEBOOK ]APPLICATION SOLUTIONS FOROLIGONUCLEOTIDES Table of ContentsIntroduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4[ HPLC & UPLC Separations ]Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideuplc, uplcacquity, acquityrnai, rnaiost, ostduplex, duplexpromass, promassduplexes, duplexessirna, sirnawaters, watersqda, qdarna, rnaanalysis, analysispairing
Application NoteLC/MS of Intact Adeno-AssociatedVirus Capsid Proteins for RapidConfirmation of Product IdentityAuthorBrian LiauAgilent Technologies, Inc.IntroductionAdeno-associated viruses (AAVs) are a promising new class of biotherapeuticcapable of treating a host of rare and intractable genetic diseases such as spinalmuscular atrophy and inherited...
Key words
deconvoluted, deconvolutedamu, amucapsid, capsidaav, aavmass, massadeno, adenorresponse, rresponsevirus, viruscounts, countsacquisition, acquisitionfld, fldassociated, associatedprotein, proteintime, timemin
 

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Application NotePharma & BiopharmaHPLC-DAD Analysis of NucleotidesUsing a Fully Inert FlowpathAgilent 1260 Infinity II Bio-Inert LC System and aPEEK-lined Agilent InfinityLab Poroshell 120 HILIC-ZColumnAuthorsKoen Sandra,Jonathan Vandenbussche,Gerd Vanhoenacker,Ruben t’Kindt, and Pat SandraResearch Institute forChromatography (RIC),President Kennedypark 26,Kortrijk, BelgiumSonja Krieger, Sonja Schneider,and...
Key words
lined, linedpeek, peekmau, mauatp, atpstainless, stainlessinert, inertadp, adpamp, ampsteel, steelresponse, responsenucleotides, nucleotidescolumn, columnctp, ctpump, umputp
Application NoteBiopharmaEvaluation of Different Ion-PairingReagents for LC/UV and LC/MSAnalysis of OligonucleotidesAuthorsGerd Vanhoenacker,Cindy Lecluyse, Griet Debyser,Pat Sandra, and Koen SandraRIC BiologicsBelgiumSonja Schipperges,Sonja Schneider, andUdo HuberAgilent Technologies, Inc.GermanyAbstractThe effect of various ion pairing reagents (amines and acidic counterions) on theLC/UV and LC/MS...
Key words
hfip, hfipmobile, mobilecounts, countsrna, rnateaa, teaaphase, phasephases, phasesons, onsalternative, alternativedna, dnaamine, aminetea, tealadder, ladderdeconvoluted, deconvoluteddad
[ APPLICATION NOTEBOOK ]APPLICATION SOLUTIONS FOROLIGONUCLEOTIDES Table of ContentsIntroduction – UPLC Analysis of Synthetic Oligonucleotides......................................................................................................................... 4[ HPLC & UPLC Separations ]Real-Time Analysis of RNAi Duplexes ....................................................................................................................................................................... 8Semi-Preparative Scale Single-Stranded RNA Purification .......................................................................................................................... 11Oligonucleotide Separation Technology: Synthesis Challenges and HPLC Isolation Options...
Key words
oligonucleotides, oligonucleotidesoligonucleotide, oligonucleotideuplc, uplcacquity, acquityrnai, rnaiost, ostduplex, duplexpromass, promassduplexes, duplexessirna, sirnawaters, watersqda, qdarna, rnaanalysis, analysispairing
Application NoteLC/MS of Intact Adeno-AssociatedVirus Capsid Proteins for RapidConfirmation of Product IdentityAuthorBrian LiauAgilent Technologies, Inc.IntroductionAdeno-associated viruses (AAVs) are a promising new class of biotherapeuticcapable of treating a host of rare and intractable genetic diseases such as spinalmuscular atrophy and inherited...
Key words
deconvoluted, deconvolutedamu, amucapsid, capsidaav, aavmass, massadeno, adenorresponse, rresponsevirus, viruscounts, countsacquisition, acquisitionfld, fldassociated, associatedprotein, proteintime, timemin
 

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