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Don’t Lose It: Getting YourPeaks in ShapeChoosing columns and conditions for thebest peak shapeGolnar JavadiColumns and Supplies Technical SupportJuly 16, 20201July 13, 2020Don’t Lose It: Getting Your Peaks in ShapeDE 5085069444 Agenda••••••2What is a good peak shape and why is it...
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factors, factorsshape, shapeaffecting, affectingpeak, peakrelated, relatedcolumn, columnendcapping, endcappingmobile, mobilebonding, bondingpore, poremetal, metalsilica, silicacomplexation, complexationtailing, tailingpacking
The Chromatography Detective:Troubleshooting Tips & Tools for LC & LCMSRita SteedLC Columns Application EngineerJanuary 31, 2018 What do you do when.....➢ Your chromatography changes• Peak shape• Retention➢ You can’t reproduce a method from• Pharmacopeia• Literature• Client• Colleague2 Areas/Things to Investigate➢ Separation•••PressurePeak ShapeRetention➢...
Key words
beginner, beginnerpitfalls, pitfallsavoiding, avoidingcolumn, columnmobile, mobilevolume, volumedwell, dwellphase, phaseecv, ecvinstrument, instrumentpeak, peakconnections, connectionsshape, shapetailing, tailingbuffer
Taking the Trouble Out of TroubleshootingIs It the Column, Method, or InstrumentRita SteedSeptember 29, 2016Page 1 What Do We TroubleshootTypical LC troubleshooting approach asks:– What’s wrong with my column?– What’s wrong with my instrument?But our separations are controlled by more than...
Key words
mobile, mobilecolumn, columnphase, phaseproblems, problemsmau, maufrit, frittailing, tailingplugged, pluggedpeaks, peaksfittings, fittingspressure, pressurepeak, peakfilter, filtershape, shapevolume
LC Column TroubleshootingIsolating the Sourceof the ProblemRita SteedDecember 12, 2013Page 1 What Do We TroubleshootThe typical LC troubleshooting approach asks the questions:– What’s wrong with the column?– What’s wrong with the instrument?oBut separations are controlled by more than just thecolumn or...
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column, columntailing, tailingmau, maumin, minvolume, volumemobile, mobilefittings, fittingsphase, phasepeak, peakplugged, pluggedtime, timepeaks, peaksfrit, fritred, redextra
 

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Don’t Lose It: Getting YourPeaks in ShapeChoosing columns and conditions for thebest peak shapeGolnar JavadiColumns and Supplies Technical SupportJuly 16, 20201July 13, 2020Don’t Lose It: Getting Your Peaks in ShapeDE 5085069444 Agenda••••••2What is a good peak shape and why is it...
Key words
factors, factorsshape, shapeaffecting, affectingpeak, peakrelated, relatedcolumn, columnendcapping, endcappingmobile, mobilebonding, bondingpore, poremetal, metalsilica, silicacomplexation, complexationtailing, tailingpacking
The Chromatography Detective:Troubleshooting Tips & Tools for LC & LCMSRita SteedLC Columns Application EngineerJanuary 31, 2018 What do you do when.....➢ Your chromatography changes• Peak shape• Retention➢ You can’t reproduce a method from• Pharmacopeia• Literature• Client• Colleague2 Areas/Things to Investigate➢ Separation•••PressurePeak ShapeRetention➢...
Key words
beginner, beginnerpitfalls, pitfallsavoiding, avoidingcolumn, columnmobile, mobilevolume, volumedwell, dwellphase, phaseecv, ecvinstrument, instrumentpeak, peakconnections, connectionsshape, shapetailing, tailingbuffer
Taking the Trouble Out of TroubleshootingIs It the Column, Method, or InstrumentRita SteedSeptember 29, 2016Page 1 What Do We TroubleshootTypical LC troubleshooting approach asks:– What’s wrong with my column?– What’s wrong with my instrument?But our separations are controlled by more than...
Key words
mobile, mobilecolumn, columnphase, phaseproblems, problemsmau, maufrit, frittailing, tailingplugged, pluggedpeaks, peaksfittings, fittingspressure, pressurepeak, peakfilter, filtershape, shapevolume
LC Column TroubleshootingIsolating the Sourceof the ProblemRita SteedDecember 12, 2013Page 1 What Do We TroubleshootThe typical LC troubleshooting approach asks the questions:– What’s wrong with the column?– What’s wrong with the instrument?oBut separations are controlled by more than just thecolumn or...
Key words
column, columntailing, tailingmau, maumin, minvolume, volumemobile, mobilefittings, fittingsphase, phasepeak, peakplugged, pluggedtime, timepeaks, peaksfrit, fritred, redextra
 

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