Waters Xevo G2-XS QTof - INSTRUMENT SPECIFICATIONS

Significance of the Topic

The advent of high–resolution accurate mass spectrometry has transformed analytical chemistry by enabling detailed qualitative and quantitative analysis of complex samples. Instruments like the Xevo G2-XS QTof deliver superior mass accuracy, dynamic range and sensitivity, supporting applications in proteomics, metabolomics, small-molecule research and quality control.

Objectives and Overview of the Instrument

This summary presents the key specifications and performance characteristics of the Xevo G2-XS QTof mass spectrometer. It highlights system hardware and software features, acquisition modes, mass ranges, resolution capabilities and sensitivity metrics that define the instrument’s analytical power.

Methodology and Instrumentation

The Xevo G2-XS QTof integrates several advanced technologies:

• StepWave™ ion optics and XS Collision Cell for robust ion transmission and MS/MS fragmentation
• High-resolution quadrupole (MS1) and oa-Tof analyser (MS2) achieving >40,000 FWHM resolution
• Versatile ZSpray™ dual-orthogonal API sources supporting ESI, APCI, APPI, nanoFlow™ ESI and other modes
• Automated vacuum system with differential pumping and digital monitoring
• IntelliStart™ software for automated system checks, calibration and sample delivery

Main Performance Characteristics and Discussion

Acquisition flexibility includes conventional MS/MS, MS E for simultaneous precursor and fragment data, FastDDA for automated data-dependent MS/MS and Tof-MRM for targeted quantitation. The mass range extends from m/z 20 to 100,000. Mass accuracy better than 1 ppm RMS and dynamic range of four orders of magnitude support trace-level detection. Sensitivity assessments demonstrate >80,000 cps for leucine enkephalin (50 pg/µL) in positive mode and >110,000 cps for raffinose (500 pg/µL) in negative mode. MS/MS sensitivity yields >7,500 cps for y-ions of [Glu1]-Fibrinopeptide B at 100 fmol/µL.

Benefits and Practical Applications

The Xevo G2-XS QTof’s combination of high resolution, mass accuracy and rapid scan rates facilitates confident compound identification, structural elucidation and quantitative analysis across pharmaceutical development, environmental monitoring, food safety and clinical research. Automated workflows ensure reproducibility and ease of use for expert and non-expert operators alike.

Future Trends and Opportunities

Ongoing developments may include enhanced ion mobility integration, expanded dynamic range technologies, real-time data processing and AI-driven acquisition strategies. Miniaturized front-ends and novel ambient ionization sources will further broaden application areas in field analysis and single-cell studies.

Conclusion

The Xevo G2-XS QTof represents a state-of-the-art platform for high-resolution mass spectrometry, combining robust hardware with intelligent software to deliver comprehensive qualitative and quantitative data. Its versatility and performance make it a valuable tool in modern analytical laboratories.

References

• Waters Corporation. Xevo G2-XS QTof Instrument Specifications. Technical Bulletin, March 2017.