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Comprehensive Characterization of the N and O-Linked Glycosylation of a Recombinant Human EPO

 

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Waters Application Notes Glycans There are a variety of complementary techniques practiced to get the complete story about a glycoprotein. Each technique varies in complexity and provides a different layer of information. Method complexity This application notebook highlights a body…
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glycan, glycanrapifluor, rapifluorglycans, glycanshilic, hilicuplc, uplcacquity, acquityamide, amideglycoworks, glycoworksflr, flrreleased, releasedunifi, unifilabeled, labeledseparations, separationsglycosylation, glycosylationfluorescence
Characterization of EPO N-Glycans using RapiFluor-MS and HILIC Profiling Rajasekar R. Prasanna, Rajiv Bharadwaj, Matthew A. Lauber, Stephan M. Koza, and Erin E. Chambers Waters India Pvt. Ltd, Bangalore, India Waters Corporation, Milford, MA, USA G OA L The analysis…
Key words
epo, epoglycans, glycansrapi, rapiglycan, glycanerythropoietin, erythropoietinfluor, fluorglycoworks, glycoworksrapifluor, rapifluorfluorescence, fluorescencelactosamine, lactosaminehilic, hilicrhepo, rhepoantennary, antennaryglycosylation, glycosylationsites
MAIN MENU TABLE OF CONTENTS [ APPLICATIONS NOTEBOOK ] BIOSEPARATIONS Tools, Techniques, and Insights into Biopharmaceutical Analysis INT RODUCTION Biopharmaceuticals have emerged as a dominant class due to their specificity and efficacy. T he production of biopharmaceuticals, however, is distinctly…
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[ GLYC AN ANALYSIS SOLUTIONS ] The Next Generation of Glycan Sample Preparation and Analysis [ GLYC AN ANALYSIS SOLUTIONS ] What if... identifying WHERE glycans are present on a glycoprotein becomes part of your routine workflow? 1 [ GLYC…
Key words
glycan, glycanglyc, glycglycoworks, glycoworksrapifluor, rapifluorglycoprotein, glycoproteinflr, flrglycans, glycansanalysis, analysisglycoprot, glycoprotintact, intactreleased, releasedsolutions, solutionshilic, hilicfluorescence, fluorescencemass
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