Waters SYNAPT XS High Resolution Mass Spectrometer

Importance of the topic

The need for rapid and flexible analytical methods is critical in scientific research to accelerate discovery and improve outcomes in diagnostics, therapeutics, and preventive measures. High sensitivity, resolution, and reproducibility are essential to unlock insights from complex samples and streamline decision-making processes.

Objectives and article overview

This document introduces the SYNAPT XS Ion Mobility Time-of-Flight Mass Spectrometer and highlights its design goals of providing ultimate flexibility, enhanced sensitivity, and reliable performance for a wide range of applications in research and industry.

Methodology and instrumentation

• Instrument architecture: StepWave XS ion guide, extended flight tube, helium cell, TriWave ion mobility module, and dual-stage reflectron.
• Data acquisition modes: SONAR, HDMSE, DIA strategies, TAP fragmentation, and HDX for structural characterization.
• Ionization and inlets: Compatibility with MALDI, DESI, ESI (nanoFlow, APCI, APGC), REIMS, ASAP, and ionKey technologies.
• Software tools: High Definition Imaging (HDI), SONAR processing, IntelliStart automated system checks, and end-to-end application solutions.

Key results and discussion

• Sensitivity comparison: The SYNAPT XS shows superior sensitivity for small molecules compared to previous generations, demonstrating higher counts per second for analytes such as acetaminophen and leu-enkephalin.
• Resolution performance: UPLC-compatible resolution exceeding 70 000 FWHM and over 80 000 in enhanced mode, resolving complex biomolecules like bovine insulin.
• Analytical dynamic range: SONAR experiments achieved linear dynamic range over 3–4 orders of magnitude in plasma protein analysis across control, asthma, and COPD samples.
• Ion mobility benefits: Collision cross section measurements and TAP fragmentation provide deeper structural insights and increased confidence in compound identification.

Benefits and practical applications

• Enhanced confidence in complex mixture analysis through complementary separation and fragmentation strategies.
• Streamlined workflows for imaging, quantitative bioanalysis, and structural elucidation using integrated software and ion mobility.
• Versatile inlet options support diverse sample types, from proteomics to metabolomics and imaging mass spectrometry.
• Automated routines and robust design reduce downtime and maintenance, ensuring reproducible results across instruments.

Future trends and opportunities

Emerging advances in data independent acquisition, high-definition imaging, and collision cross section libraries will expand the ability to characterize complex samples. Integration with artificial intelligence for data mining and real-time decision support promises to further accelerate discovery and enhance laboratory productivity.

Conclusion

The SYNAPT XS platform delivers a flexible, high-performance solution for a broad spectrum of analytical challenges. Its combination of sensitivity, resolution, ion mobility, and versatile acquisition modes positions it as a key tool to drive innovation, support regulatory compliance, and advance scientific discovery across disciplines.

Used Instrumentation

• SYNAPT XS Ion Mobility Time-of-Flight Mass Spectrometer
• StepWave XS segmented quadrupole ion guide
• TriWave ion mobility separation module
• Dual-stage reflectron and extended flight tube
• Helium collision cell
• Variety of inlet sources: ESI, MALDI, DESI, APCI, APGC, REIMS, ASAP, ionKey.