Does Tween 20 Affect Monoclonal Antibody Separation?
Applications | 2017 | Agilent TechnologiesInstrumentation
GPC/SEC
IndustriesPharma & Biopharma
ManufacturerAgilent Technologies
Key wordsdimer, monomer, igg, factor, after, ratio, aggregations, performance, aggs, aggregate, factors, min, multiple, injections, test, before, study, column, evalution, protein, remain, duong, mapp, keeley, plates, profiles, phu, bio, components, nriu, eluent, were, dimers, rad, separation, start, elution, change, containing, unchanged, monomers, biosimilars, remained, aggregates, conditioned, biotherapeutics, injecting, agilent, between, little
Similar PDF
Aggregate and Fragment Analysis - Agilent BioHPLC Columns Application Compendium
2020|Agilent Technologies|Guides
Agilent-NISTmAbAggregate andFragment AnalysisAgilent BioHPLC ColumnsApplication Compendium ContentsAgilent-NISTmAb Standard (P/N 5191-5744; 5191-5745) was aliquoted fromNISTmAb RM 8671 batch. Quality control (QC) testing is performed usingAgilent LC-MS system. QC batch release test includes aggregate profile, chargevariants and intact mass information. A certificate of...
Key words
page, pagecontents, contentssec, secback, backmobile, mobilebuffer, bufferexclusion, exclusionmau, maunacl, naclmonomer, monomerhwm, hwmdimer, dimerphase, phasesize, sizeresolution
Agilent AdvanceBio SEC Columns for Aggregate Analysis: Instrument Compatibility
2017|Agilent Technologies|Technical notes
Agilent AdvanceBio SEC Columnsfor Aggregate Analysis: InstrumentCompatibilityTechnical OverviewIntroductionAgilent AdvanceBio SEC columns are a new family of size exclusion chromatography(SEC) columns packed with 2.7 µm particles of a unique, low binding, polymer coatedsilica stationary phase. This technology provides minimal nonspecific interactions,and...
Key words
aggs, aggsprotein, proteinadvancebio, advancebiodimer, dimercalibrate, calibratemonomer, monomermau, mausec, secmin, mindead, deadcolumns, columnsangiotensin, angiotensincolumn, columnperformance, performanceregularly
Helpful ‘Hints’ for Protein SEC Success
2021|Agilent Technologies|Presentations
Helpful ‘Hints’ for ProteinSEC SuccessJean LaneApplications EngineerLC columns and consumables technical supportMay 27, 20201 Outline for TalkGetting started•SEC definition, common terminology, and why use it?•Considerations for selecting an SEC column- Pain Points/Challenges for SEC- Importance of Pore Size and Particle Size...
Key words
hints, hintshelpful, helpfulsec, secsuccess, successprotein, proteinpore, poreadvancebio, advancebiosize, sizebuffer, bufferexclusion, exclusionmobile, mobilephase, phasecolumn, columndimer, dimervolume
Agilent Biocolumns - Aggregate/Fragment Analysis - Application Compendium
2021|Agilent Technologies|Guides
Agilent BiocolumnsAggregate/Fragment AnalysisApplication Compendium ContentsBackground2Getting Started3How to Guide - Size Exclusion Chromatography for BiomoleculeAnalysis - 5991-3651EN 4Featured Application NotesElevate Your mAb Aggregate Analysis - 5994-2709EN12222High-Resolution, High-Throughput Size Exclusion ChromatographyAnalysis of Monoclonal Antibodies - 5994-0828EN29Sensitive Native Mass Spectrometry of MacromoleculesUsing Standard Flow LC/MS...
Key words
return, returnsection, sectionmau, maucontents, contentssec, secinsulin, insulinmin, minprotein, proteinsize, sizeadvancebio, advancebiomonomer, monomertime, timeexclusion, exclusionaggregates, aggregatesaggregate