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Parallel accumulation – serial fragmentation combined with data-independent acquisition (diaPASEF)

 

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diaPASEF: label-free quantification of highly complex proteomes The timsTOF Pro with diaPASEF provides reproducible and accurate qualitative and quantitative results in complex proteomics samples – making it perfectly suited for data-independent acquisition approaches. Abstract Data-independent acquisition (DIA) promises reproducible and…
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dia-PASEF® applied on different gradient lengths Data Independent Acquisition (DIA) workflows have gained in popularity as they overcome the issue of stochastic selection of peptide precursors encountered in typical data-dependent approaches (DDA) and thereby promise reproducible and accurate protein identification…
Key words
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High throughput 4D-Proteomics – Application of dia-PASEF® and the Evosep One for short gradients The timsTOF Pro offers a combination of two unique technologies, namely a 4th dimension provided by Trapped Ion Mobility Spectrometry (TIMS) to enhance ion separation and…
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High throughput proteomics – Application of dia-PASEF and Evosep One for short gradients ASMS 2020, MP122 Stephanie Kaspar-Schoenefeld1, Markus Lubeck1, Thomas Kosinski1, Scarlet Koch1, Oliver Raether1, Gary Kruppa1 1Bruker Daltonik GmbH, 28359 Bremen, Germany Introduction Data-independent acquisition (DIA) promises reproducible…
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