Agilent G1734AA MassHunter Forensic Toxicology Dynamic MRM Database Kit

Significance

The MassHunter Forensic Toxicology Dynamic MRM Database Kit streamlines multi-analyte screening in forensic laboratories by integrating optimized MRM transitions and adaptive retention time windows into a single LC-MS workflow.
By reducing method development time and enhancing sensitivity, it supports high-throughput detection of up to 200 toxicology targets in a single run.

Objectives and Study Overview

This guide outlines the setup and application of the Agilent G1734AA database kit.
It includes installation of software and hardware, preparation of a 25-component test mix, execution of dynamic MRM methods, and generation of custom assays.

Methodology and Instrumentation

An Agilent 1200 Series LC equipped with Binary Pump SL (mixer and damper bypassed), wellplate sampler, and ZORBAX Eclipse Plus C18 column (2.1×100 mm, 1.8 μm) is coupled to a 6400 Series Triple Quadrupole LC/MS.
MassHunter Data Acquisition B.02.01 or higher, Quantitative Analysis B.03.01+, Qualitative Analysis B.03.00+, and Optimizer B.02.01+ handle data collection, processing, and method optimization.
Dynamic MRM enables data acquisition only during predefined retention time windows, maximizing dwell time and signal quality.
Mobile phases consist of 5 mM ammonium formate/0.01% formic acid in water (A) and 0.01% formic acid in acetonitrile (B).

Main Results and Discussion

A 10 ppb test mix of 25 forensic analytes demonstrated clear chromatographic separation over a 5.5-minute gradient.
Extracted chromatograms revealed segmented MRM windows aligned with compound retention times, improving peak definition and quantitation precision.
Custom MRM methods were generated by importing database transitions and retention data, then converted to Dynamic MRM using report-based retention time tables.

Benefits and Practical Applications

• Rapid deployment of comprehensive forensic toxicology assays
• Enhanced sensitivity through optimized fragmentor and collision energies
• Higher throughput via dynamic retention scheduling
• Reduced instrument idle time and improved data quality

Real-world applications include drug screening, postmortem toxicology, and workplace safety testing.

Future Trends and Applications

Expanding dynamic databases to cover new drug classes and emerging toxins
Integration with automated sample preparation platforms for end-to-end workflows
Application of machine learning to refine retention time predictions and transition selection
Real-time feedback loops for adaptive method adjustments and instrument control

Conclusion

The MassHunter Forensic Toxicology Dynamic MRM Database Kit offers a validated, efficient pathway for multiplexed toxicology screening, minimizing development time while maximizing sensitivity and throughput.

Instrumentation Used

• Agilent 1200 Series LC (Binary Pump SL, Column Compartment, Wellplate Sampler)
• ZORBAX Eclipse Plus C18 column 2.1×100 mm, 1.8 μm
• Agilent 6400 Series Triple Quadrupole LC/MS
• MassHunter Data Acquisition, Quantitative Analysis, Qualitative Analysis, and Optimizer software

References

• Technical Note on Dynamic MRM, p/n 5990-3595EN
• Technical Note on Pesticide Dynamic MRM Database, p/n 5990-4255EN