Thermo Scientific Vanquish Split Samplers - PRODUCT SPECIFICATIONS

Significance of the Topic

High-precision sample injection is a cornerstone of reliable liquid chromatography, directly impacting retention time reproducibility, quantitative accuracy and overall laboratory throughput. In modern HPLC and UHPLC workflows, advanced split-sampler designs enhance productivity by minimizing carryover, automating sample handling and ensuring robust performance under a wide range of pressures and solvent conditions.

Objectives and Overview

This application note details the performance specifications and operational capabilities of the Thermo Scientific Vanquish Split Samplers, designed to maximize injection precision, sample capacity and method transfer ease across various system configurations (Horizon, Flex, Core and Duo). Emphasis is placed on throughput enhancement, reproducibility and integration with chromatography data systems.

Methodology and Instrumentation

The Vanquish Split Samplers employ SmartInject technology combined with an optimized fluidic path and air-stream cooling to preserve sample integrity. Key instrumentation elements include:

• Split-loop injection principle for precise micro- to milliliter-scale volumes
• Integrated barcode reading for automated sample identification and inventory tracking
• Customizable gradient delay volume to streamline method transfer across different modules
• Optional dual-injection (Duo) configuration to double throughput

Main Features and Discussion

Performance data show:

• Pressure tolerance up to 151 MPa (Horizon) or 103 MPa (Flex) depending on model
• Injection volumes from 0.01 to 1,000 µL with sub-percent area precision and linearity (r>0.99999)
• Carryover below 0.002% (caffeine test) and rapid cycle times down to 8 s
• Sample compartment temperature control between 4 °C and 40 °C for thermally sensitive analyses

Materials in sample and eluent flow paths (e.g. MP35N, titanium, ceramics, PEEK) ensure chemical compatibility and biocompatibility over wide pH and chloride ranges.

Benefits and Practical Applications

The Vanquish Split Samplers deliver:

• Enhanced reproducibility for QC/QA, pharmaceutical assays and environmental testing
• Flexible sample formats (vials, well plates) supporting high-throughput screening
• Reduced downtime via predictive maintenance functions in Chromeleon CDS audit trails

Future Trends and Potential Applications

Advances in sampler design will likely focus on further miniaturization, integration with online sample prep and expansion of biocompatible materials. Coupling split samplers with automated workflows and machine-learning-driven scheduling promises additional gains in throughput and data quality.

Conclusion

Thermo Scientific Vanquish Split Samplers set a new benchmark in injection precision, robustness and automation for both routine HPLC and high-performance UHPLC applications, supporting laboratories in achieving greater productivity and analytical confidence.