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Size and Purity Assessment of Single-Guide RNAs by Anion-Exchange Chromatography (AEX)

 

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RNA Analysis for CRISPR by capillary electrophoresis with laser-induced fluorescence detection CE-LIF Jane Luo, Tingting Li, Marcia Santos, Fang Wang, Sahana Mollah and Handy Yowanto SCIEX Separations, Brea, California Introduction CRISPR (clustered regularly interspaced short palindromic repeats) is an immunological…
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Application Note Separation and Size Assessment of dsDNA Fragments by Anion-Exchange Chromatography (AEX) Hua Yang, Stephan M. Koza, Weibin Chen Waters Corporation Abstract Reliably separating dsDNA species and assessing their sizes are important analytical tasks in the fields of cell…
Key words
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Application Note Cursory Analysis of Nucleic Acid Components in Adeno-Associated Virus (AAV) Samples Using Anion-Exchange Chromatography (AEX) Hua Yang, Stephan M. Koza, Ying Qing Yu Waters Corporation Abstract It is important to analyze adeno-associated virus (AAV) therapeutics for contaminating nucleic…
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Characterizing LNP mRNA
2024|Waters|Guides
CONSUMABLES GUIDE BOOK Characterizing LNP mRNA LC tools for purity, identity, integrity and concentration determining measurements A Changing Industry mRNA based medicine helped address the COVID-19 pandemic. The viability of using an mRNA construct as a human drug substance has…
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