2019/2021 Chromatography Consumables Catalog - Bio LC columns and accessories

Significance of Connected Chromatography Solutions

Chromatography productivity and data reproducibility depend on a fully integrated workflow. From sample preparation and vial integrity to column chemistry and detection methods, each consumable element contributes to reliable results and reduced reanalysis costs.

Objectives and Overview

This catalog presents a comprehensive portfolio of chromatography consumables designed to deliver connected solutions across five major application areas: biopharma/pharma, food & beverage, environmental, clinical research, and general proteomics. It enables method selection by detailing consumables for sample handling, columns of varied chemistries and formats, and accessory options.

Methodology and Instrumentation

Workflows combine:

• Sample preparation: SPE plates and cartridges (HyperSep, SOLAµ), SLE, QuEChERS, protein digests (SMART Digest) and filtration devices.
• Chromatography modes: reversed phase (C4, C8, C18), ion exchange (WCX, SCX, SAX, WAX), size exclusion (SEC), hydrophobic interaction (HIC), HILIC and mixed-mode (anion or cation exchange plus RP).
• Column formats: analytical to guard, nano, capillary, microbore and semipreparative, monolithic and core-shell.
• Detection: UV/Vis, fluorescence, charged aerosol detection, ELSD and mass spectrometry (Orbitrap, Exactive, TSQ).

Main Results and Discussion

The catalog highlights application-specific solutions:

• Biopharma: MAbPac columns for mAb charge variants (SCX), size exclusion (SEC-1), hydrophobic interaction (HIC-10/20/Butyl) and affinity (Protein A, IMAC, ConA monoliths).
• Pharma: HyperSep SPE and QuEChERS for small-molecule sample prep, ProPac IEX for peptide/protein DMPK, and HPLC/GC columns for QA/QC.
• Food & Beverage: Pesticide and mycotoxin workflows using SPE, QuEChERS, GC consumables and LC columns (aQ, Carbamate, VDX).
• Environmental: SPE and monolithic SPME Arrow for POPs, SVOCs, VOCs, with TraceGOLD GC columns and liners.
• Clinical research: SPE, SLE, LC columns for proteomics, metabolomics and drug monitoring.

Benefits and Practical Applications

By selecting matched consumables for each step—sample preparation, chromatography and detection—laboratories achieve:

• Improved method robustness and reproducibility.
• Higher instrument uptime and reduced carryover.
• Scalable solutions from analytical to preparative scale.
• Streamlined method development with preconfigured kits and pH gradient buffers.

Used Instrumentation

Key instrumentation includes Thermo Scientific UHP/UHPLC systems (Vanquish, Dionex UltiMate 3000), bio-compatible autosamplers, EASY-Spray and nanoViper fitted nanoLC emitters, Orbitrap and triple-quadrupole mass spectrometers, and Chromeleon™ chromatography data system.

Future Trends and Potential Applications

Emerging directions include:

• Further integration of automation and robotics in sample prep.
• AI-driven method scouting and predictive maintenance.
• Expansion of micro- and nanoflow LC-MS for single-cell and ultra-low-volume analyses.
• Advanced hybrid columns combining multiple retention mechanisms for multidimensional separations.

Conclusion

A connected consumables and column portfolio is essential for modern chromatography workflows. Thermo Fisher’s integrated solutions support a broad range of applications, ensuring high performance, reproducibility and workflow efficiency.

References

• Harris RJ, Bertschinger J, Vlasak J. Characterization of monoclonal antibody variants by cation exchange chromatography. J Chromatogr A. 1995;705:129–134.