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Application Note No. 88 Targeted Proteomic Analysis of Central Metabolic Enzymes Using Nano-LC-Triple Quadrupole Mass Spectrometry Fumio Matsuda1,2,3, Takafumi Iwakura1, Taisuke Seike1 and Yutaka Umakoshi4 Life Science Life Sciences Central metabolic pathways also play a role in the ability of…
Key words
central, centralenzymes, enzymesmetabolic, metabolicexpression, expressionnano, nanotargeted, targetedproteomics, proteomicscerevisiae, cerevisiaemrm, mrmmass, massstarkeyi, starkeyimetabolism, metabolismlipomyces, lipomycespathways, pathwaystrypsin
Application Note No. 87 Using Targeted Proteomics with an UltraFast Triple Quadrupole Mass Spectrometer to Confirm Protein Overexpression Fumio Matsuda1,2,3 and Yutaka Umakoshi4 Life Science Life Sciences  Abstract 1. Introduction Protein overexpression is an experimental approach that is utilized…
Key words
pgk, pgkoverexpression, overexpressionprotein, proteintrypsin, trypsinpeptides, peptidesoverexpress, overexpressmrm, mrmdigested, digestedskyline, skylineproteomics, proteomicsmicroorganisms, microorganismstriple, tripleexpression, expressiontransitions, transitionsselectively
Magnetic Resonance Mass Spectrometry (MRMS) discriminates yeast mutants through metabolomics An untargeted metabolomics approach based on the MRMS aXelerate® workflow was employed to examine changes in methylglyoxal catabolism using Saccha­romyces cerevisiae as a model system. This approach allowed for subtle…
Key words
methylglyoxal, methylglyoxalglyoxalase, glyoxalasemrms, mrmsglutathione, glutathionemutants, mutantsphenotypically, phenotypicallystrains, strainsmetabolomics, metabolomicsgene, genecerevisiae, cerevisiaesaccharomyces, saccharomycesmass, masspathway, pathwayuntargeted, untargetedwere
C146-E280D Selection Guide Metabolite Analysis Metabolomics Product Portfolio Expanding Metabolomics Metabolomics refers to an array of techniques used to comprehensively detect and analyze various metabolites formed in vivo during biological activity. The qualitative and quantitative changes in metabolites reflect the…
Key words
acid, acidsba, sbametabolites, metabolitesdatabase, databasepackage, packageacids, acidsmetabolomics, metabolomicsphospholipid, phospholipidanalysis, analysisamino, aminomediators, mediatorsmonophosphate, monophosphatecooh, coohlcms, lcmsmrm
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