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Confident LC-MS Identification of Low ppm Host Cell Proteins (HCPs) in Biotherapeutic Monoclonal Antibodies

 

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Deep Identification and Quantitation of Unknown HCPs in NISTmAb Using an Optimized Sample Preparation Combined with NanoLC-MS Jianan.Sun1; Yue.Zhou2; Tao.Bo1; 1Thermo Fisher Scientific, Beijing, China; 2Thermo Fisher Scientific, Shanghai, China ABSTRACT RESULTS Individual HCP quantitation Purpose: To develop and demonstrate…
Key words
nanolc, nanolchcps, hcpsexactive, exactiveantibody, antibodythermo, thermodigestion, digestionpreparation, preparationscientific, scientificplus, plusaccession, accessionhcp, hcpkai, kaibeijing, beijinghumanized, humanizedprotein
APPLICATION NOTE 74162 LC-MS-based host cell protein (HCP) identification and monitoring during biopharmaceutical downstream process development Authors: Xiaoxi Zhang1, Zijuan Chen2, Bingnan Li2, Jennifer Sutton3, Min Du4 Thermo Fisher Scientific 1 Shanghai, China; 2 Bioprocess Design Center, Shanghai, China; 3…
Key words
hcp, hcppool, poolhcps, hcpscex, cexabundance, abundancerelative, relativeaex, aexprotein, proteinpeptide, peptidedownstream, downstreammab, mabphospholipase, phospholipaseprocess, processppm, ppmhost
APPLICATION NOTE 000242 Adeno-associated virus capsid protein characterization and host cell protein profiling using micro-flow UHPLC-Orbitrap MS Authors: Reiko Kiyonami1, Jennifer Sutton1, Katy Irvin2, Ken Cook3, Estee Toole4, Min Du5 Thermo Fisher Scientific, San Jose, CA; 2 Thermo Fisher Scientific,…
Key words
hcp, hcpaav, aavpeptide, peptideprotein, proteinpurified, purifiedviral, viralcapsid, capsidmapping, mappingharvest, harvestmodification, modificationsetting, settingpepsin, pepsinabundant, abundanthost, hostproteins
APPLICATION NOTE 21918 Easy, fast and reproducible analysis of host cell protein (HCP) in monoclonal antibody preparations Authors Giorgio Oliviero1, Ken Cook 2, Kai Scheffler3, Florian Füssl1, Jonathan Bones1 1National Institute for Bioprocessing Research and Training, Dublin, Ireland; 2Thermo Fisher…
Key words
hcp, hcppeptide, peptidemapping, mappinghcps, hcpshost, hostbead, beadsmart, smartkingfisher, kingfishersetting, settinglane, lanecell, celldigest, digestcharge, chargemab, mabanalysis
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