Analysis of Pyridine

Significance of the Topic

Analyzing pyridine is crucial due to its prevalence in pharmaceuticals environmental monitoring and chemical manufacturing Its toxicological profile and strict regulatory limits necessitate reliable detection and quantification in various sample matrices

Objectives and Study Overview

This application note demonstrates a robust reversed-phase HPLC method for the selective separation and quantification of pyridine alongside uracil and phenol using the Shim-pack GIS CN column The focus is on achieving clear resolution under isocratic conditions with minimal sample preparation

Methodology and Instrumentation

• Column Shim-pack GIS CN 150 mm L × 4.6 mm I D 5 µm
• Mobile phase Water (A) and Methanol (B) mixed 70∶30 v/v
• Flow rate 1.0 mL/min
• Column temperature 40 °C
• Detection UV at 254 nm

Main Results and Discussion

The optimized isocratic method delivered baseline separation of uracil phenol and pyridine with sharp symmetric peaks Reproducibility of retention times and consistent peak areas confirmed the method’s robustness The cyanopropyl stationary phase provided distinctive selectivity for the basic pyridine moiety preventing coelution and matrix interferences

Benefits and Practical Applications

• Fast and reproducible analysis suitable for QA/QC laboratories
• Simplified mobile phase composition reduces preparation time
• Applicable to pharmaceutical purity testing environmental and industrial samples

Future Trends and Opportunities

Integration with mass spectrometric detection could enhance sensitivity for trace-level pyridine analysis Development of green solvents and adoption of smaller column formats promises faster run times lower solvent consumption and reduced environmental impact Automation and online sample preparation are likely to streamline high-throughput workflows

Conclusion

The presented reversed-phase HPLC method using Shim-pack GIS CN column provides a simple reliable and selective approach for pyridine determination Its robustness and versatility make it a valuable tool for ensuring product quality regulatory compliance and environmental safety