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Fast Online Desalting of mAbs Using a Reversed-Phase Desalting Cartridge for LC-MS Analysis

Applications | 2016 | Thermo Fisher ScientificInstrumentation
LC/HRMS, LC/MS, LC/MS/MS, LC/Orbitrap
Industries
Pharma & Biopharma
Manufacturer
Thermo Fisher Scientific
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TECHNICAL NOTE Authors: Martin Samonig, Sabrina Patzelt, Martin Rühl, and Remco Swart Thermo Fisher Scientific, Germering, Germany Key words Pre-concentration, trap and elute, desalt, MSPac, MAbPac, monoclonal antibody, mAb, Vanquish, reversed phase, mass spectrometry, Q Exactive, biopharmaceutical, biomolecules, intact protein…
Key words
pump, pumploading, loadingvanquish, vanquishcolumn, columnrituximab, rituximabtrap, trapvtcc, vtccexactive, exactivelactalbumin, lactalbuminanalytical, analyticalmspac, mspacmass, massribonuclease, ribonucleaseprotein, proteinsample
Martin Samonig, Remco Swart Thermo Fisher Scientific, Germering, Germany Appli cat i on N ote 1 1 3 3 SEC-MS with Volatile Buffers for Characterization of Biopharmaceuticals Key Words Monoclonal Antibodies, MAbPac SEC-1, Biocompatible UHPLC, Biotherapeutics Characterization, Biopharma Goal Prove…
Key words
abundance, abundancerelative, relativesec, secflex, flexmass, massbuffers, buffersvanquish, vanquishmonoclonal, monoclonalprotein, proteinexclusion, exclusionthermo, thermosize, sizescientific, scientifictfa, tfasystem
Application Note Biologics Efficient Monoclonal Antibody and Antibody Drug Conjugate Desalting Prior to Mass Spectrometry Using AdvanceBio Desalting-RP Cartridges Authors Isabel Vandenheede, Pat Sandra, and Koen Sandra Research Institute for Chromatography (RIC) President Kennedypark 26 B-8500 Kortrijk Anne Blackwell and…
Key words
herceptin, herceptincounts, countsdesalting, desaltingdeconvoluted, deconvolutedpapain, papainamu, amuidez, idezadvancebio, advancebiomass, masslinker, linkercartridge, cartridgedtt, dttdigested, digestedfab, fabmabs
Martin Samonig1,2, Christian Huber1,2 and Kai Scheffler2,3 1 Division of Chemistry and Bioanalytics, University of Salzburg, Salzburg, Austria 2 Christian Doppler Laboratory for Innovative Tools for Biosimilar Characterization, University of Salzburg, Salzburg, Austria 3 Thermo Fisher Scientific, Dreieich, Germany Key…
Key words
abundance, abundanceeeqynstyr, eeqynstyrintact, intactrelative, relativechain, chainantibody, antibodymonolithic, monolithiclight, lightmonoclonal, monoclonalmass, massheavy, heavyaif, aiffragmentation, fragmentationmonoisotopic, monoisotopictrypsin
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