Introducing HPLC solutions from Thermo Fisher Scientific
Summary
Significance of Advanced HPLC Solutions
High-performance liquid chromatography (HPLC) continues to be an essential technique for separating, identifying and quantifying complex mixtures in pharmaceutical, environmental, biochemical and industrial laboratories. The integration of ultra-high-pressure capabilities, micro- and nano-flow options, advanced detectors and intelligent software addresses growing demands for higher throughput, greater resolution and robust reproducibility.
Objectives and Overview
This summary examines the comprehensive HPLC and UHPLC product portfolio from Thermo Fisher Scientific, highlighting system configurations from basic binary pumps to dual UHPLC/nano-flow platforms. It describes how modular hardware, high-frequency detectors and automated workflows combine to enhance analytical capabilities and commercial reach.
Methodology and Instrumentation
- UltiMate 3000 series: Fully UHPLC compatible modules (basic, standard, dual LC, RSLC and RSLCnano) with binary/quaternary pumping up to 1,000 bar (up to 8 mL/min) and optional dual-stream operation.
- Detectors: 100–200 Hz diode arrays (DAD, VWD, MWD), fluorescence (FLD), coulometric (ECD) and charged aerosol (CAD) for sensitive, broad-spectrum detection.
- Accela 1250/600: High-pressure pumps up to 1,250 bar (2 mL/min) or 600 bar (5 mL/min) with Force Feedback Control for stable baselines without pulse dampeners.
- EASY-nLC series: Nano-LC systems (EASY-nLC II at 300 bar, EASY-nLC 1000 at 1,000 bar) supporting 1D/2D separations, intelligent maintenance and remote support.
- Viper™ and nanoViper fittings: Finger-tight, zero dead-volume connectors compatible with standard capillaries up to 1,200 bar, simplifying micro- and nano-flow system assembly.
- Chromeleon 7 CDS: Streamlined chromatography data system offering automated method scouting, application switching, parallel/tandem LC control and enterprise-level data management.
Main Results and Discussion
Thermo Fisher’s UHPLC+ platform demonstrates effective scaling from analytical to nano-scale flows. Dual-pump configurations double throughput and support advanced techniques such as online SPE, parallel/tandem LC and 2D separations. High-pressure operation (up to 1,250 bar) combined with sub-2 µm column compatibility significantly reduces analysis time and improves peak capacity. Finger-tight Viper fittings eliminate dead volume, enhancing reproducibility in nano-LC workflows. Integrated software tools automate method development and data processing, reducing human error and accelerating project timelines.
Benefits and Practical Applications
- Scalable solutions from routine QA/QC to high-resolution proteomics and metabolomics.
- Enhanced peak resolution and faster run times through UHPLC compatibility and high-frequency detection.
- Ergonomic design and low maintenance requirements improve laboratory productivity.
- Automated workflows and comprehensive software control minimize downtime and facilitate regulatory compliance.
Future Trends and Opportunities
The convergence of high-pressure nano-LC with cutting-edge mass spectrometry, AI-driven method optimization and cloud-based data analytics will expand the frontiers of single-cell analysis, lipidomics and real-time process monitoring. Continued miniaturization and greener solvent practices will support sustainable laboratory operations and broaden applications in point-of-care diagnostics.
Conclusion
Thermo Fisher’s integrated HPLC portfolio offers a versatile, end-to-end solution for diverse analytical challenges. By combining robust high-pressure hardware, advanced detection technologies, ergonomic fittings and intelligent software, laboratories can achieve higher throughput, superior data quality and seamless workflow integration across multiple disciplines.
Content was automatically generated from an orignal PDF document using AI and may contain inaccuracies.
Introducing HPLC solutions from
Thermo Fisher Scientific
2
Proprietary & Confidential
An Exciting Combination!
More
Capabilities
+
=
Chromatography
Powerhouse
Integrated LC/MS
Solutions
Commercial Reach
& Deep Expertise
#3 in Chromatography ~$650M.
On our way to #1 by 2020
IC, HPLC, UHPLC, Nano HPLC,
GC, sample prep, software and
consumables
Leader in MS becomes a leader in
LCMS
LCMS & GCMS including single
quad, tandem (QQQ & ion trap)
and hybrid techniques
350,000 Customers
150 Countries
37,000 Employees
Increased presence in Asia-Pacific
Deep Applications Expertise
3
Proprietary & Confidential
All UltiMate 3000 Systems Now Fully UHPLC Compatible
Basic
Automated
Highly economic & reliable
620 bar UHPLC compatible
Flow rates up to 10 mL/min
100 Hz detector range
Modular flexibility
Standard
3rd Generation Modules
620 bar UHPLC compatible
Flow rates up to 10 mL/min
Oven temp. 5 – 80 ºC
100 Hz DAD, MWD, VWD, FLD,
CAD
Highest flexibility
x2 Dual LC
Two systems in one
620 bar UHPLC compatible
Flow rates up to 10 mL/min
Oven temp. 5 – 80 ºC
Automated Application Switching
Parallel and Tandem LC
Online SPE-LC
Automated method scouting
Turn key Viper kits for ease of use
RSLC
Binary and Quaternary UHPLC
systems
1000 bar up to 5 mL/min
800 bar up to 8 mL/min
Oven temp. 5 – 110 ºC
200 Hz DAD, MWD, VWD, FLD
Improved sub 2-µm particle column
compatibility
Ultrafast/ultra resolution system
x2 Dual RSLC
x2 Dual UHPLC System
Two systems in one
1000 bar up to 5 mL/min
800 bar up to 8 mL/min
Oven temp. 5 – 110 ºC
200 Hz DAD, MWD, VWD, FLD
Parallel and Tandem LC
Online SPE-LC
Automated method scouting
Offline 2D-UHPLC
Turn key Viper kits for ease of use
RSLCnano
UHPLC system for Nano/Cap/Micro
range
20 nL/min – 50 µL/min up to 800 bar
Continuous direct flow
New standard in retention time
precision
Snap-in valves
nanoViper fitting system for easy
operation
Basic
Standard
x2 Dual LC
RSLC
x2 Dual RSLC
RSLCnano
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Proprietary & Confidential
Dionex’ Industry Leading UHPLC Portfolio
Thermostatted
Standard
+ Fractionation
Nano/Cap Flow
Basic
Quaternary
Dual-Gradient
Binary
Nano/Cap Flow
Isocratic
With Valves
High Temp.
Viper/nanoViper
Standard
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Proprietary & Confidential
Dionex’ UHPLC-Compatible Detectors
VWD
MWD
DAD
FLD
Coulochem III (ECD)
Corona Ultra (CAD)
The most complete UHPLC detector range
100Hz VWD-3100
200Hz VWD-3400RS
100Hz MWD-3000SD
200Hz MWD-3000RS
100Hz DAD-3000SD
200Hz DAD-3000RS
100Hz FLD-3100
200Hz FLD-3400RS
100Hz Corona Ultra (CAD)
100Hz Coulochem III (ECD)
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Proprietary & Confidential
Accela Instruments
Accela increases laboratory productivity, through its versatility with
conventional and high speed chromatographic capabilities in an
efficient, easy to use system that handles pressures up to 1250bar.
Accela 1250 and Accela 600
•
Maximum operating pressure of 1,250bar across the entire flow
rate range up to 2ml/min (Accela 1250)
• Maximum operating pressure of 600bar across the entire flow
rate range up to 5ml/min (Accela 600)
• Extremely low pump delay volume
• Quaternary capability
• Equipped with Force Feedback Control (FFC) technology to
provide extremely stable baselines without the use of pulse
dampeners and greatest flow rate and gradient accuracy without
the need of sensors that are in contact with the mobile phase
7
Proprietary & Confidential
EASY-nLC Product Line
• EASY-nLC II
• 300 bar
• EASY-nLC 1000
• 1000 bar
• ASMS launch!
• Common Benefits
• Unrivalled ease of use
• Effortless operation
• Proven performance with
state-of-the-art MS systems
• Excellent productivity
- high resolution and reproducibility
• Excellent resolution and
reproducibility in complex samples
• 1D and 2D nano-LC in one system
• Intelligent maintenance
• Remote support
• Easy maintenance, access and
service
8
Proprietary & Confidential
Viper™ Finger Tight Fitting System
• Finger tight, correct fit every time!
• Universal (fits to Rheodyne, Valco, Swagelok...)
• No dead volume as soon as tightly connected
• Ease of use, no sleeve for small capillaries
• 1200 bar compatible
Ease of Use and Ergonomic Design
PEEK Seal
Removable knurl
Adapter (no ferrule!)
Finger Tight Screw
1/32” capillary
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Proprietary & Confidential
Viper™ Performance
ZERO Dead Volume
Standard finger tight fitting at 600 bar (8,700 psi)
VIPER at 600 bar (8,700 psi)
10 Proprietary & Confidential
Ease of Use – nanoViper
● nanoViper is nano LC made easy !
● Features
● Fingertight connection
● Up to 1000 bar
● Dead volume free by design
● Values
● Tool-free assembly, easy fluidics
● No column damage by over-tightening
● UHPLC compatible
● No experimental failure due to bad connections
11 Proprietary & Confidential
Viper Application Tubing Kits
Ready to Use Viper UHPLC+ Solution Kit:
•
All Viper Capillary parts
•
Laminated card with flow schemes
•
Quick installation guide
•
Operation Instructions
•
Template files for CM 6 and CM 7
12 Proprietary & Confidential
UHPLC+ x2 Dual Applications Summary
Tandem LC
Online SPE
Parallel LC
Application Switching
Automated Method Scouting
13 Proprietary & Confidential
Chromeleon 7 – Simply Intelligent
13
14 Proprietary & Confidential
Chromatography Process
Chromatography Ends
with Results
Chromatography Starts
with Samples
Chromeleon 7
Streamlines the
Steps from Samples to
Results
16 Proprietary & Confidential
UHPLC+ Focused LC Product Portfolio
Basic
Automated
Standard
LC
RSLC
RSLCnano
CMS Software
Viper Fitting
System
Acclaim
Columns
Corona ultra
17 Proprietary & Confidential
Document Outline
- Introducing HPLC solutions from Thermo Fisher Scientific
- An Exciting Combination!
- All UltiMate 3000 Systems Now Fully UHPLC Compatible
- Dionex’ Industry Leading UHPLC Portfolio
- Dionex’ UHPLC-Compatible Detectors
- Accela Instruments
- EASY-nLC Product Line
- Viper™ Finger Tight Fitting System
- Viper™ Performance
- Ease of Use – nanoViper
- Viper Application Tubing Kits
- UHPLC+ x2 Dual Applications Summary
- Chromeleon 7 – Simply Intelligent
- Chromatography Process
- UHPLC+ Focused LC Product Portfolio
- Slide Number 17
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