Orbitrap IQ-X Tribrid mass spectrometer - PRODUCT SPECIFICATIONS

Significance of the Topic

High-resolution mass spectrometry plays a central role in small molecule identification and characterization across pharmaceutical, environmental and metabolomics research. Advanced instrumentation that integrates multiple analyzers and real-time data analysis workflows addresses critical challenges in sensitivity, selectivity and throughput. The Orbitrap IQ-X Tribrid mass spectrometer represents a step forward in unlocking structural details of unknown compounds and improving confidence in complex sample analysis.

Objectives and Overview

The primary aim is to present the key design features, performance enhancements and application capabilities of the Orbitrap IQ-X Tribrid system. This summary outlines the study objectives, highlights methodological approaches, summarizes main performance data and discusses practical benefits for small molecule workflows.

Instrumental Setup

The system combines three main analyzers in a Tribrid architecture and offers flexible ion sources and optional modules:

• Quadrupole mass filter for high-efficiency precursor selection
• Dual-pressure linear ion trap for rapid MSn acquisition and CID fragmentation
• High-field Orbitrap mass analyzer delivering up to 500 000 resolution at m/z 200 (1 000 000 option)
• OptaMax NG electrospray ion source with interchangeable HESI and APCI probes
• Auto-Ready calibration source and EASY-IC for sub-ppm mass accuracy
• Optional UVPD laser source, 1M resolution module and FAIMS Pro Duo interface

Main Results and Discussion

Performance testing demonstrates ultra-high resolution up to 500 000 FWHM at m/z 200 with isotope fidelity and options for 1 000 000 resolution. Acquisition rates reach 40 Hz in the Orbitrap and 45 Hz in the ion trap. Mass accuracy remains below 1 ppm RMS with internal calibration. The system supports up to 10-plex targeted MS2 and SIM using the ion-routing multipole. Real-Time Library Search enables dynamic MSn triggering based on spectral matching against a local mzVault library. AcquireX workflows automate inclusion and exclusion lists for deep profiling, improving coverage of low-abundance species.

Benefits and Practical Applications

Key advantages include enhanced unknown compound identification, reduced method development time and streamlined maintenance via automated calibration. Application templates for metabolomics, lipidomics, impurity profiling and extractables and leachables accelerate deployment in research, QAQC and regulated environments. The combined fragmentation modes CID, HCD and UVPD at any MSn stage enable comprehensive structural elucidation.

Future Trends and Possibilities

Upcoming directions involve integration of machine learning for automated data interpretation, expansion of high-confidence spectral libraries and coupling with advanced ion mobility separations. Further speed improvements and higher-throughput autosampler interfaces will support large cohort studies and real-time process monitoring.

Conclusion

The Orbitrap IQ-X Tribrid mass spectrometer delivers a versatile platform for small molecule analysis, balancing ultra-high resolution, flexible fragmentation and intelligent acquisition workflows. Its capabilities meet the evolving demands of structural characterization and unknown identification in diverse analytical fields.

References

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