Installation Instruction for ProfIC Vario 15 Anion

Importance of the topic

IC is a fundamental separation technique for monitoring inorganic anions in environmental, industrial, and research applications. The Metrohm ProfIC Vario 15 Anion system combines sequential suppression with the intelligent Partial Loop Technique (MiPT) to automate routine analyses, enabling high sensitivity and precision in quantifying anions from µg/L to mg/L without manual dilution.

Aims and overview of the bulletin

This document provides a detailed, step-by-step procedure for installing and configuring the ProfIC Vario 15 Anion system and its software. Key objectives include:

• Setting up the hardware components for automatic anion determination
• Installing and configuring MagIC Net software for instrument control
• Establishing the injection and calibration strategy using MiPT

Methodology and instrumentation

Installation and configuration cover both hardware and software aspects:

• Hardware assembly of the 940 Professional IC Vario, 858 Professional Sample Processor, and 800 Dosino
• Connection of capillaries, suppressor, and detector using MiPT accessories
• Software installation of MagIC Net 3.0 and automatic recognition of devices via USB
• Definition of needle positions, solution properties, and rack configuration in MagIC Net
• Purge and conditioning of the suppressor and separation column prior to analysis
• Method creation: single-standard multipoint calibration by varying injection volumes

Used Instrumentation

• 940 Professional IC Vario One/SeS/PP with Conductivity Detector (2.850.9010)
• 858 Professional Sample Processor (2.858.0010) with Swing Head and Liquid Handling Station
• 800 Dosino (2.800.0010) for solution delivery
• MiPT kit including suppressor cartridges (MSM-HC, MSM-LC), Metrosep A and C columns
• MagIC Net software version 3.0
• Optional 941 Eluent Production Module for automated eluent generation

Main results and discussion

An exemplary chromatographic separation of seven anions (F⁻, Cl⁻, NO₂⁻, Br⁻, NO₃⁻, SO₄²⁻, PO₄³⁻) was performed:

• Injection volume: 8 µL; concentration: 2 mg/L per analyte
• Calibration range: 1–50 mg/L using a quadratic fit; correlation coefficients ≥0.99994
• Precision: 10 replicate injections at 1 mg/L yielded relative standard deviations below 1% for all ions

Benefits and practical applications of the method

• Fully automated workflow reduces manual intervention and error
• No prior dilution needed through MiPT, streamlining sample preparation
• Single-standard multipoint calibration saves time and reagents
• Reliable performance for QA/QC, environmental monitoring, and process control

Future trends and opportunities

Emerging developments include:

• On-line eluent generation for continuous, unattended operation
• Advanced suppression technologies to lower detection limits
• Integration of AI-driven diagnostics for predictive maintenance
• Modular and hyphenated configurations coupling IC with sample-preparation and spectroscopic detectors

Conclusion

The ProfIC Vario 15 Anion system with MiPT offers a robust, high-throughput solution for anion analysis. Its automated hardware and software integration deliver consistent, precise results while minimizing manual handling and reagent use.

Reference

No external literature references were provided in the original document.