Agilent Captiva syringe filters and disposable syringes - Ordering Guide

Importance of the Topic

Sample filtration is a critical preparatory step in chromatographic workflows. Efficient removal of particulates prevents backpressure spikes, retention-time shifts, resolution loss and premature column wear. High-performance syringe filters streamline routine analysis in pharmaceutical, environmental and biochemical laboratories, improving data quality and instrument uptime.

Study Objectives and Overview

This document introduces Agilent Captiva syringe filters and matching disposable syringes. It aims to demonstrate how these products:

• Deliver rapid flow rates and high loading capacity
• Minimize protein binding for biological samples
• Exhibit very low extractables under LC and LC/MS conditions

Performance comparisons against competing filters highlight advantages in filtration efficiency and column lifetime.

Methodology and Instrumentation

Filtration performance was assessed using a 0.05% suspension of 0.3 µm latex beads. A sub-2 µm reversed-phase analytical column served to evaluate backpressure over multiple injections of both unfiltered and filtered samples. Filtration efficiency across 10 replicates was measured for Agilent filters and benchmarked against other suppliers. Instrumentation used:

• Agilent Captiva syringe filters (0.2 µm and 0.45 µm pore sizes; nylon, PES, PTFE, RC, cellulose acetate and glass microfiber membranes)
• Agilent Captiva disposable syringes (various volumes, luer lock and slip options)
• HPLC system with sub-2 µm column and pressure monitoring

Main Results and Discussion

Filtration dramatically extended column life: filters reduced backpressure buildup over 1 000 injections compared with unfiltered samples. Agilent Captiva filters achieved average efficiencies above 98% for 0.2 µm nylon membranes, outperforming competitor products at both 0.2 µm and 0.45 µm ratings. The low extractable profile of PES filters ensured minimal baseline disturbance in LC/MS assays, while the industry’s lowest protein binding preserved analyte recovery in bioanalytical workflows.

Benefits and Practical Applications

Key advantages of Agilent Captiva syringe filters and syringes include:

• Increased laboratory throughput via fastest flow rates
• Extended column lifetimes and reduced maintenance costs
• Reliable performance in LC and LC/MS methods, including proteomics and metabolomics
• Wide membrane selection supports aqueous, organic and biological sample types
• Convenient pack sizes and compatible disposable syringes streamline workflows

Future Trends and Applications

Emerging needs in high-throughput and automated analysis will drive further innovation in filtration technology. Anticipated developments include integrated filter-syringe-vial systems, advanced multilayer membranes for broad-spectrum particle removal, and compatibility with robotic sample handlers. Sustainability trends may also prompt biodegradable or recyclable filter housings.

Conclusion

Agilent Captiva syringe filters and matching disposable syringes offer a robust, versatile solution for sample cleanup ahead of chromatographic analysis. Their superior flow performance, low extractables and protein-friendly membranes translate into higher data quality, lower operational costs and increased instrument uptime.

Reference

Agilent Technologies Captiva Syringe Filters and Disposable Syringes Ordering Guide, 2022.